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Full-Text Articles in Genetics and Genomics
Application Of Dna Fingerprints For Cell-Line Individualization, Dennis A. Gilbert, Yvonne Reid, Mitchell H. Gail, David Pee, Christine White, Robert J. Hay, Stephen J. O'Brien
Application Of Dna Fingerprints For Cell-Line Individualization, Dennis A. Gilbert, Yvonne Reid, Mitchell H. Gail, David Pee, Christine White, Robert J. Hay, Stephen J. O'Brien
Biology Faculty Articles
DNA fingerprints of 46 human cell lines were derived using minisatellite probes for hypervariable genetic loci. The incidence of 121 HaeIII DNA fragments among 33 cell lines derived from unrelated individuals was used to estimate allelic and genotypic frequencies for each fragment and for composite individual DNA fingerprints. We present a quantitative estimate of the extent of genetic difference between individuals, an estimate based on the percentage of restriction fragments at which they differ. The average percent difference (APD) among pairwise combinations from the population of 33 unrelated cell lines was 76.9%, compared with the APD in band sharing …
Dna Recombination And Natural Selection Pressure Sustain Genetic Sequence Diversity Of The Feline Mhc Class I Genes, Naoya Yuhki, Stephen J. O'Brien
Dna Recombination And Natural Selection Pressure Sustain Genetic Sequence Diversity Of The Feline Mhc Class I Genes, Naoya Yuhki, Stephen J. O'Brien
Biology Faculty Articles
Sequence comparisons of seven distinct MHC class I cDNA clones revealed that feline class I molecules have a remarkable similarity to human HLA genes in their organization of functional domains as well as in the nonrandom partitioning of genetic variability according to the functional constraints ascribed to different regions of the MHC molecule. The distribution of the pattern of sequence polymorphism in the cat as compared with genetic diversity of human and mouse class I genes provides evidence for four coordinate factors that contribute to the origin and sustenance of abundant allele diversity that characterizes the MHC in the species. …
Prevalence And Implications Of Feline Coronavirus Infections Of Captive And Free-Ranging Cheetahs (Acinonyx Jubatus), J. L. Heeney, J. F. Evermann, A. J. Mckeirnan, L. Marker-Kraus, M. E. Roelke, M. Bush, D. E. Wildt, D. G. Meltzer, L. Colly, J. Lukas, V. J. Manton, T. Caro, Stephen J. O'Brien
Prevalence And Implications Of Feline Coronavirus Infections Of Captive And Free-Ranging Cheetahs (Acinonyx Jubatus), J. L. Heeney, J. F. Evermann, A. J. Mckeirnan, L. Marker-Kraus, M. E. Roelke, M. Bush, D. E. Wildt, D. G. Meltzer, L. Colly, J. Lukas, V. J. Manton, T. Caro, Stephen J. O'Brien
Biology Faculty Articles
The extent and progression of exposure to feline infectious peritonitis (FIP) virus in the cheetah, Acinonyx jubatus, was monitored by a world-wide serological survey with indirect fluorescent antibody titers to coronavirus. The indirect fluorescent antibody assay was validated by Western blots, which showed that all indirect fluorescent antibody-positive cheetah sera detected both domestic cat and cheetah coronavirus structural proteins. There was a poor correlation between indirect fluorescent antibody results and the presence of coronaviruslike particles in cheetah feces, suggesting that electron microscopic detection of shed particles may not be an easily interpreted diagnostic parameter for FIP disease. Low, but …