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Full-Text Articles in Genetics and Genomics
Phenotype Characterization Of Sas-7(Or1945) C. Elegans, Chase Reinert
Phenotype Characterization Of Sas-7(Or1945) C. Elegans, Chase Reinert
Student Symposium
The ability to form a bipolar spindle is crucial for accurate cell division. In the nematode C. elegans several genes have been described with roles in spindle assembly including sas-7. The centriole is a key organizer of mitotic spindles. The sas-7 protein is a centriole component that regulates centriole duplication, elongation, and assembly. To date, most work on sas-7 was using a conditional non-null allele. In this study, phenotypes associated with the loss-of-function sas-7(or1945) null allele were characterized. Homozygous sas-7(or1945) hermaphrodites have reduced brood sizes with no viable embryos compared to wild-type and heterozygotes. When they do produce embryos, they …
Invasion Genetics Of The Non-Native Geckos Phelsuma Grandis Gray 1870 And Gekko Gecko (Linnaeus 1758) In Southern Florida, Usa, Thomas William Fieldsend
Invasion Genetics Of The Non-Native Geckos Phelsuma Grandis Gray 1870 And Gekko Gecko (Linnaeus 1758) In Southern Florida, Usa, Thomas William Fieldsend
FIU Electronic Theses and Dissertations
Biological invasions cause tremendous damage to ecosystems, economies, and human livelihoods worldwide. Florida is home to more established non-native species of reptiles and amphibians than anywhere else on Earth, many of which cause substantial harm to native biodiversity and human well-being. The relatively new discipline of invasion genetics promises to significantly improve the understanding, prediction, prevention, and management of biological invasions. The purpose of this dissertation is to utilize invasion genetics techniques to further understanding of the patterns and processes of biological invasions, especially as they pertain to Florida’s destructive and diverse non-native squamate reptile assemblage. In the first phase …
Population Genetic Analyses Of Arctic Char (Salvelinus Alpinus) Life History Types In Nettilling Lake & Amadjuak River Ecosystem: A Test Of Reproductive Isolation, Chen Liu
Electronic Theses and Dissertations
A great number of studies have identified strong genetic differences between sympatric anadromous and resident populations of Salmonidae. However, Arctic char (Salvelinus alpinus) migratory phenotypes in the Nettilling Lake and Amadjuak River ecosystem in Nunavut, Canada have not been genetically characterized, and it remains unclear if distinct genotypes and phenotypes associated with migratory life history differences are maintained through reproductive isolation, and they have been assumed to be sympatric populations, or co-occurring populations. Co-occurring Arctic char (n=225) were sampled from eleven sites along the Amadjuak River in 2014 and 2015. Twelve microsatellite loci were used to quantify genetic variation among …
Will There Be Changes In Sexually Differentiated Behaviors In Mice Manipulated For The Sry Gene As They Mature Into Adultood?, Subin Joo
CMC Senior Theses
Sexually differentiated behavior has been shown to be affected by both genes and hormones. The discovery of the SRY gene, which codes for the development of testes, lead to the development of the Four Core Genotypes model of mice, and the separation of sex chromosomes and its resulting gonadal hormones. Using the FCG model, this study aims to look at the development of sexually differentiated behavior in mice, and track how it changes throughout their life. FCG mice will be divided into individual and social housing, and repeated experiments carried out to test their reaction to both intact female and …
Connections In The Underworld: A Morphological And Molecular Study Of Diversity And Connectivity Among Anchialine Shrimp., Robert Eugene Ditter
Connections In The Underworld: A Morphological And Molecular Study Of Diversity And Connectivity Among Anchialine Shrimp., Robert Eugene Ditter
FIU Electronic Theses and Dissertations
This research investigates the distribution and population structure of crustaceans, endemic to anchialine systems in the tropical western Atlantic focusing on cave-dwelling shrimp from the family Barbouriidae. Taxonomic and molecular tools (genetic and genomic) are utilized to examine population dynamics and the presence of phenotypic hypervariation (PhyV) of the critically endangered species Barbouria cubensis (von Martens, 1872). The presence of PhyV and its geographic distribution is investigated among anchialine populations of B. cubensis from 34 sites on Abaco, Eleuthera, and San Salvador, Bahamas. Examination of 54 informative morphological characters revealed PhyV present in nearly 90% (n=463) of specimens with no …
Genetic Analysis Of The Endangered Gray Bat Species (Myotis Grisescens), Emma Fitzgerald
Genetic Analysis Of The Endangered Gray Bat Species (Myotis Grisescens), Emma Fitzgerald
Lewis Honors College Capstone Collection
This study attempts to analyze the genetics using specific haplotype sequences of the endangered gray bat to determine genetic events that have happened in the past as well as determining how the species population has changed over time. We were able to conduct a Tajima’s D test to investigate whether a potential bottleneck has occurred. A linear regression of the genetic vs. geographical distance was produced to investigate the patterns of haplotype distribution. The species past distribution was compared to the current distribution using known collected specimens. A map of future predictions was constructed using present and future climate layers …
Associations Of Single Nucleotide Polymorphisms In The Bovine Prolactin, Melatonin Receptor 1a, And Dopamine Receptor D2 Genes With Hair Coat Shedding Scores And Productivity Traits In Beef Cattle, Laura R. Meyer
Graduate Theses and Dissertations
Calving rate is a qualitative trait regulated by several genes and is strongly affected by the environment. With the development of biotechnology and gene identification, scientists are able to determine which genes affect these productivity traits to improve accurate selection decisions. Prolactin (PRL) has been associated with reproductive traits, melatonin receptor 1a (MTNR1A) has been associated with meat quality traits, and the dopamine receptor D2 (DRD2) gene has been associated with hair coat score (HCS) in cattle. Our objective was to determine associations between mutations in the PRL, MTNR1A, and DRD2 genes and cow-calf profitability traits. Genomic DNA was extracted …
Dna Sequence Of Melanocortin 1-Receptor Gene In Coturnix Japonica: Correlation With Three E Locus Alleles—E,E+, And Erh, Rupali Ugrankar, Kim Cheng, Ronald Okimoto
Dna Sequence Of Melanocortin 1-Receptor Gene In Coturnix Japonica: Correlation With Three E Locus Alleles—E,E+, And Erh, Rupali Ugrankar, Kim Cheng, Ronald Okimoto
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
The melanocortin 1-receptor (MC1-R) gene plays a key role in the expression of fur and feather color in mammals and birds by regulating the distribution of two melanin pigments: eumelanin (black/brown) and pheomelanin (red/yellow). MC1-R corresponds to the classical Extension (E) locus in mice, pigs, dogs, horses, and chickens. Three E locus alleles, the wild-type (e+), brown (E), and redhead (erh) have been identified in Japanese quail (Coturnix japonica). To determine if the quail E locus phenotypes were due to variation in the MC1-R gene, the coding region of the MC1-R gene was PCR amplified and DNA sequenced using genomic …