Open Access. Powered by Scholars. Published by Universities.®

Genetics and Genomics Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 11 of 11

Full-Text Articles in Genetics and Genomics

Uncovering Tasselsheath3. A Genomic And Phenotypic Analysis Of A Maize Floral Mutant., Thompson Zhang Oct 2017

Uncovering Tasselsheath3. A Genomic And Phenotypic Analysis Of A Maize Floral Mutant., Thompson Zhang

Masters Theses

In the modern era, maize has become the most successful crop grown in the United States. According to the USDA over 90 million acres of land are planted to corn and 96.2% of the U.S feed grain production is made up of the cereal. Part of the success of maize is due to its floral architecture, and its pollination technique in which the flower opens, exposing stamens containing pollen into the air. A unique organ called the lodicule functions as a release mechanism, forcing the flower to open. Lodicules from grasses and eudicot petals are homologous, yet there is little …


Characterization Of Acaricide Resistance, Plant-Mediated Rnai Against Two-Spotted Spider Mites (Tetranychus Urticae Koch), And Assessing Off- And Non-Target Effects, Hooman Hosseinzadeh Namin Oct 2017

Characterization Of Acaricide Resistance, Plant-Mediated Rnai Against Two-Spotted Spider Mites (Tetranychus Urticae Koch), And Assessing Off- And Non-Target Effects, Hooman Hosseinzadeh Namin

Electronic Thesis and Dissertation Repository

The two-spotted spider mite (TSSM), Tetranychus urticae (Koch), is one of the most damaging agricultural pests in the world. It feeds on over 150 crops, causing considerable yield losses in greenhouses and agricultural fields. Currently, using synthetic acaricides is the main method to control TSSM. However, it can develop resistance to acaricides with repeated exposure, and typically resistance can occur within two to four years. To understand the underlying mechanisms of spider mite adaptation to acaricides is an essential part of resistance management strategy. The resistance ratio of the pyridaben-selected strain compared with the pre-selection strain was estimated at greater …


The Key Question In Symbiotic Nitrogen Fixation: How Does Host Maintain A Bacterial Symbiont?, Onur Oztas Jul 2017

The Key Question In Symbiotic Nitrogen Fixation: How Does Host Maintain A Bacterial Symbiont?, Onur Oztas

Doctoral Dissertations

The fact that plants cannot use nitrogen in the gaseous form makes them dependent on the levels of usable nitrogen forms in the soil. Legumes overcome nitrogen limitation by entering a symbiotic association with rhizobia, soil bacteria that convert atmospheric nitrogen into usable ammonia. In root nodules, bacteria are internalized by host plant cells inside an intracellular compartment called the symbiosome where they morphologically differentiate into nitrogen-fixing forms by symbiosome-secreted host proteins. In this project, I explained the host proteins required to maintain bacterial symbionts and described their delivery to the symbiosome. I showed that the SYNTAXIN 132 (SYP132) gene …


The Population Genetics Of Morro Bay Eelgrass (Zostera Marina), Julia Gardner Harencar Jun 2017

The Population Genetics Of Morro Bay Eelgrass (Zostera Marina), Julia Gardner Harencar

Master's Theses

Seagrass populations are in decline worldwide. Zostera marina (eelgrass), one of California’s native seagrasses, is no exception to this trend. In the last 8 years, Morro Bay, California has lost 95% of its eelgrass. Eelgrass is an ecosystem engineer, providing important ecosystem services such as sediment stabilization, nutrient cycling, and nursery habitats for fish. The failure of recent restoration efforts necessitates a better understanding of the causes of eelgrass decline in this estuary. Previous research on eelgrass in California has demonstrated a link between population genetic diversity and eelgrass bed health, ecosystem functioning, and resilience to disturbance and extreme climatic …


Differentiating The Neches River Rose Mallow (Hibiscus Dasycalyx) From Its Congeners By Means Of Phylogenetics And Population Genetics, Julia Norrell May 2017

Differentiating The Neches River Rose Mallow (Hibiscus Dasycalyx) From Its Congeners By Means Of Phylogenetics And Population Genetics, Julia Norrell

Biology Theses

This study used molecular phylogenetic methods to attempt to resolve the taxonomic status of the federally threatened East Texas-endemic wildflower, the Neches River Rose Mallow (Hibiscus dasycalyx). Hibiscus dasycalyx co-occurs with two other closely related congeners that are currently not of conservation concern: the halberdleaf rose mallow (H. laevis); and the crimson-eyed rose mallow (H. moscheutos). This study assessed the phylogeny of these three Hibiscus species, and attempted to determine if there is possible hybridization occurring between them. To this end, Restriction Site Associated DNA Sequencing (RAD-Seq), a Next Generation Sequencing method, was used …


Phenotypic Variation And Genetic Purity Of The Original 'Prime-Jim®' X 'Arapaho' Population, Loren M. Luther May 2017

Phenotypic Variation And Genetic Purity Of The Original 'Prime-Jim®' X 'Arapaho' Population, Loren M. Luther

Horticulture Undergraduate Honors Theses

In 2013, Castro et al., produced the first linkage map for primocane-fruiting blackberries using the cross ‘Prime-Jim®’ x ‘Arapaho.’ This mapping population has been maintained since the original experiment with the hopes of conducting future studies. Further research was determined to be needed on the population to better characterize additional phenotypic traits, and since nine years had passed since the seedlings were established, there was a need to assess the continued genetic purity of the population using molecular methods. Phenotypic data was collected to analyze the variation of the population including soluble solids content, titratable acidity, average weight, shape, and …


Gatekeepers Of Nitrogen-Fixing Symbiosis: Cytokinin-Ethylene Crosstalk Regulates Symbiotic Interaction In Lotus Japonicus, Seyedehmandana Miri Apr 2017

Gatekeepers Of Nitrogen-Fixing Symbiosis: Cytokinin-Ethylene Crosstalk Regulates Symbiotic Interaction In Lotus Japonicus, Seyedehmandana Miri

Electronic Thesis and Dissertation Repository

Leguminous plants thrive under nitrogen-limited soil conditions because of their ability to symbiotically interact with nitrogen-fixing bacteria, known as rhizobia. In the presence of compatible strains of rhizobia, they develop specialized symbiotic organs, called root nodules, which host the bacteria and provide the appropriate conditions for symbiotic nitrogen fixation to occur. The plant hormone cytokinin is the key endogenous trigger for the inception of root nodule organogenesis. In the model legume Lotus japonicus, analysis of the cytokinin receptor gene Lotus histidine kinase 1 (Lhk1) showed that it is required and also sufficient for the initiation of nodule …


Examining The Role Of Grp And Lik1 In Wall Associated Kinase (Wak) Perception Of Pectin In The Plant Cell Wall, Jack Ryan Mitchell Jan 2017

Examining The Role Of Grp And Lik1 In Wall Associated Kinase (Wak) Perception Of Pectin In The Plant Cell Wall, Jack Ryan Mitchell

Honors Projects

Wall associated kinases (WAKs) are cell membrane bound receptor kinases that bind pectin and pectin fragments (OGs).The binding of WAKs to pectin sends a growth signal required for cell elongation and plant development. WAKs bind OGs with higher affinity than native pectin and instead activate a stress response. Glycine rich proteins (GRPs) are secreted cell wall proteins of unknown function. Seven GRPs with 65% sequence similarity are coded on a 90kb locus of Arabidopsis chromosome 2. GRP3 and WAK1 have been shown to bind in vitro, but single null mutations have no discernible phenotype, suggesting that the GRPs are redundant. …


Micro-Spectroscopy Of Bio-Assemblies At The Single Cell Level, Jeslin Kera Jan 2017

Micro-Spectroscopy Of Bio-Assemblies At The Single Cell Level, Jeslin Kera

Honors Undergraduate Theses

In this thesis, we investigate biological molecules on a micron scale in the ultraviolet spectral region through the non-destructive confocal absorption microscopy. The setup involves a combination of confocal microscope with a UV light excitation beam to measure the optical absorption spectra with spatial resolution of 1.4 μm in the lateral and 3.6 μm in the axial direction. Confocal absorption microscopy has the benefits of requiring no labels and only low light intensity for excitation while providing a strong signal from the contrast generated by the attenuation of propagating light due to absorption. This enables spatially resolved measurements of single …


Responding To Soil Fungal Communities: A Look At Interactions Between Arbuscular Mycorrhizal Fungi And The Common Yellow Monkeyflower, Mariah Mcintosh Jan 2017

Responding To Soil Fungal Communities: A Look At Interactions Between Arbuscular Mycorrhizal Fungi And The Common Yellow Monkeyflower, Mariah Mcintosh

Undergraduate Theses, Professional Papers, and Capstone Artifacts

The obligate fungal mutualists arbuscular mycorrhizal fungi (AMF) colonize the roots approximately 80% of vascular plants, generally thought to provide mineral nutrition, pathogen protection, or drought resistance to plants in exchange for photosynthetic carbon. Because of the ecological and evolutionary significance of these interactions, much work has been done to understand this symbiosis at the community level. However, much remains to be understood about how AMF affect plant fitness on an individual level. In this study, we took advantage of the tractability of the emerging model species Mimulus guttatus, the common yellow monkeyflower, to identify genetic differences in how …


Araucaria In The Urban Landscape: A Novel Leaning Pattern And Evidence Of Cultivated Hybridization, Jason W. Johns Jan 2017

Araucaria In The Urban Landscape: A Novel Leaning Pattern And Evidence Of Cultivated Hybridization, Jason W. Johns

Master's Theses

Our understanding of the natural world is constantly evolving and strengthening as more observations are made and experiments are performed. For example, we understand that tree stems grow toward the light (positive phototropism; Darwin 1880, Loehle 1986, Christie et al. 2013) and against gravity (negative gravitropism; Knight 1806, Hashiguchi et al. 2013). We also know that plants respond to mechanical stimulus and perturbation (thigmotropism; Braam 2005). Genes and their resulting proteins have been described to uncover some of the mechanisms for these environmental responses, but relatively speaking, we have just scratched the surface (Wyatt et al. 2013). While the discovery …