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Full-Text Articles in Genetics and Genomics
Sensitive And Specific Detection Of Sars-Cov-2 In Saliva Using Reverse Transcriptase Loop-Mediated Isothermal Amplification, Casey Juntila
Sensitive And Specific Detection Of Sars-Cov-2 In Saliva Using Reverse Transcriptase Loop-Mediated Isothermal Amplification, Casey Juntila
All NMU Master's Theses
A novel coronavirus called SARS-CoV-2 has caused the emergency release of the most well-known molecular assay, the CDC N1 RT-PCR assay, causing reports of poor analytical performance resulting in false-negative results (20, 26), and inconsistent testing kits received (23, 32). An immediate and dire need for a rapid and reliable SARS-CoV-2 testing workflow specifically designed for a university setting is the purpose this project is aiming and intended to fulfill. The workflow design uses a less invasive saliva sample for rapid screening using colorimetric RT-LAMP detection of three SARS-CoV-2 gene regions for Orf1ab, envelope, and nucleocapsid. Purified SARS-CoV-2 genomic RNA …