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Full-Text Articles in Genetics and Genomics
Identification Of A Genetic Marker For Litter Size In Sheep, Olivia Price
Identification Of A Genetic Marker For Litter Size In Sheep, Olivia Price
Undergraduate Honors Capstone Projects
The purpose of this experiment was to determine if the estrogen receptor gene (ESR) could be used as a genetic marker for litter size in sheep. The estrogen receptor gene was chosen because it has been successfully used as a genetic marker for litter size in swine. In this study, DNA dilutions from two experimental flocks, totaling approximately 200 animals, were used. Amplification of exon 1/intron 1 of the ESR gene was performed using PCR. Initial results indicated a polymorphism in the gene when cut with the restriction enzyme Ava ll. It appeared that the gene contained a deletion with …