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Biochemistry, Biophysics, and Structural Biology
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Full-Text Articles in Genetics and Genomics
An Approach To Identify Mycobacteriophage Diversity Prior To Dna Sequencing, Charles Gregory
An Approach To Identify Mycobacteriophage Diversity Prior To Dna Sequencing, Charles Gregory
Mahurin Honors College Capstone Experience/Thesis Projects
Over 6,869 Mycobacteriophages have been isolated and purified. Of these, 1,367 genomes have been sequenced at the DNA level and more are added each year through the SEA-PHAGES program. Sequenced mycobacteriophages are grouped into clusters based on a 50% or greater nucleotide identity. The number and breadth of these clusters represents the diversity present in the environment. Each year, as new phages are discovered by students in the SEA-PHAGES program, the question arises, “Which isolates should we sequence?” In order to sequence phages that represent the greatest possible diversity, and thus broaden under-represented clusters and identify new singletons, we need …