Genetics and Genomics Commons^™

Genetic Polymorphisms Of The Glucocorticoid Receptor And Interleukin-8 Receptor Genes Are Related To Production Traits And Hair Coat Score In Crossbred Cattle, Avery Deaton

Animal Science Undergraduate Honors Theses

The objective of this thesis was to identify polymorphisms in the glucocorticoid receptor (GR) and interleukin-8 receptor (CXCR2) genes and to associate genotypes between the above mentioned polymorphisms and production traits in crossbred cattle. The hypothesis was that polymorphisms will exist for GR and CXCR2 genes and will be linked to production traits. Glucocorticoid receptors have been positively associated with higher milk yields, lactose content, feed intake, and feed conversion rates. Interleukin-8 genes are part of the innate immune response and help with many aspects of female reproduction health, such as protecting the embryo from the maternal immune system during …

Associations Of Single Nucleotide Polymorphisms In The Bovine Prolactin, Melatonin Receptor 1a, And Dopamine Receptor D2 Genes With Hair Coat Shedding Scores And Productivity Traits In Beef Cattle, Laura R. Meyer

Graduate Theses and Dissertations

Calving rate is a qualitative trait regulated by several genes and is strongly affected by the environment. With the development of biotechnology and gene identification, scientists are able to determine which genes affect these productivity traits to improve accurate selection decisions. Prolactin (PRL) has been associated with reproductive traits, melatonin receptor 1a (MTNR1A) has been associated with meat quality traits, and the dopamine receptor D2 (DRD2) gene has been associated with hair coat score (HCS) in cattle. Our objective was to determine associations between mutations in the PRL, MTNR1A, and DRD2 genes and cow-calf profitability traits. Genomic DNA was extracted …

Examination Of Polymorphism In The Serine Proteinase Inhibitor Gene In The Eastern Oyster, Crassostrea Virginica., Aine Lehane

Senior Honors Projects

Oysters are a keystone species; they are filter-feeders and reef-builders. Additionally, oysters are extremely important within the shellfish aquaculture industry; they are the second most widely produced species in world aquaculture production. Eastern oysters, Crassostrea virginica, are produced in aquaculture and are unfortunately susceptible to Dermo disease. Dermo is caused by a protozoan parasite, Perkinsus marinus, and infection is often fatal in oysters due to widespread tissue damage and blockage of blood vessels. Research in oyster disease is particularly needed because disease is one of the largest impediments faced by the aquaculture industry as entire oyster populations can …

Similar Levels Of X-Linked And Autosomal Nucleotide Variation In African And Non-African Populations Of Drosophila Melanogaster, Nadia D. Singh, J. Michael Macpherson, Jeffrey D. Jensen, Dmitri A. Petrov

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Background: Levels of molecular diversity in Drosophila have repeatedly been shown to be higher in ancestral, African populations than in derived, non-African populations. This pattern holds for both coding and noncoding regions for a variety of molecular markers including single nucleotide polymorphisms and microsatellites. Comparisons of X-linked and autosomal diversity have yielded results largely dependent on population of origin.

Results: In an attempt to further elucidate patterns of sequence diversity in Drosophila melanogaster, we studied nucleotide variation at putatively nonfunctional X-linked and autosomal loci in sub-Saharan African and North American strains of D. melanogaster. We combine our experimental …

A Sex-Linked Allele, Autosomal Modifiers And Temperature-Dependence Appear To Regulate Melanism In Male Mosquitofish (Gambusia Holbrooki), Lisa Horth

Biological Sciences Faculty Publications

About 1% of male mosquitofish (Gambusia holbrooki) express melanic (mottled-black) body coloration, which differs dramatically from the wild-type, silvery-gray coloration. Here, I report on the genetic inheritance pattern of melanic coloration, which indicates Y-linkage, and at least one autosomal modifier. Phenotypic expression of melanism is also affected by temperature. Expression is constitutive ( temperature insensitive) in some populations, inducible ( temperature sensitive) in others. Constitutive and inducible expression occur among geographically proximal populations. However, males from any single population demonstrate the same constitutive or inducible expression pattern as one another. The F1 males from inter-population crosses demonstrate temperature-related …

Rapid Communication: Mapping Of The Beta Tropomyosin (Tpm2) Gene To Pig Chromosome 1, J. Sherwood, Daniel C. Gill, M. F. Rothschild

Department of Animal Science: Faculty Publications

Locus. Pig Tropomyosin beta (TPM2) gene.

Genus and Species. Sus Scrofa.

Source and Description of Primers. Primers were derived by using well-conserved regions of published human and mouse sequence (GenBank accession nos. AF209746 and NM_009416). The primers were used to amplify a 1.4-kp fragment of the porcine TPM2 gene covering exons 3 through 8. The porcine cDNA sequence (GenBank accession no. AF420022) showed 95% exonic identity (325 bp) at the nucleotide level to the human skeletal muscle beta tropomyosin (GenBank accession no. X06825). Additionally, pig specific primers were designed to amplify a 246-bp piece that spans exons 3 to …

Rapid Communication: Mapping Of The Ca2+ Atpase Of Fast Twitch 1 Skeletal Muscle Sarcoplasmic Reticulum (Atp2a1) Gene To Porcine Chromosome 3, Daniel C. Ciobanu, Y. Ciobanu, M. F. Rothschild

Department of Animal Science: Faculty Publications

Locus Name. Ca²⁺ ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum.

Species. Sus scrofa.

Locus Symbol. ATP2A1.

Source of Primers. Two sets of PCR primers were designed based on human ATP2A1 sequences (Gen- Bank accession numbers U96773 and U96777) available in the GenBank database. Using pig genomic DNA as a template we used set A primers to amplify approximately 1,100 bp spanning exon 1 to exon 2 and set B primers to amplify 1,200 bp gene segment covering some of exon 8 through part of exon 9. These sequences were deposited in GenBank, accession number AY027797-AY027799.