Open Access. Powered by Scholars. Published by Universities.®

Genetics and Genomics Commons

Open Access. Powered by Scholars. Published by Universities.®

Agronomy and Crop Sciences

Starch gel electrophoresis

Publication Year

Articles 1 - 3 of 3

Full-Text Articles in Genetics and Genomics

Additional Mapping Of Isozyme Loci: Localization Of Acp4, Dia2, Adkl, Tpi3, And Sadl, Jonathan F. Wendel, Major M. Goodman, Charles W. Stuber Jan 1986

Additional Mapping Of Isozyme Loci: Localization Of Acp4, Dia2, Adkl, Tpi3, And Sadl, Jonathan F. Wendel, Major M. Goodman, Charles W. Stuber

Jonathan F. Wendel

We recently listed the isozyme loci being studied by starch gel electrophoresis in our laboratory and summarized the available mapping data in a series of reports (Wendel et al., MGCNL 59:87-90). Subsequent work has resulted in further clarification of the chromosomal locations of markers on chromosomes 1 (Acp4, Dia2), 6 (Adkl), 8 (Tpi3), and 10 (Sadl). Previously unreported information on these loci and their chromosomal locations follows.


Localization Of Two New Isozyme Loci, Hexl And Tpi4, To Chromosome 3, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman Jan 1985

Localization Of Two New Isozyme Loci, Hexl And Tpi4, To Chromosome 3, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

Recent work with starch gel electrophoresis of coleoptile extracts has indicated that Hexl, the structural locus for the most anodal set of hexokinase isozymes, and Tpi4, which encodes the slowest migrating set of triose phosphate isomerase bands, are located on chromosome 3.


Linkage Relationships Between A New Locus, Hex2, And Previously Assigned Loci On Chromosome 6, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman Jan 1985

Linkage Relationships Between A New Locus, Hex2, And Previously Assigned Loci On Chromosome 6, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

Starch gel electrophoresis of maize coleoptile extracts in our laboratory has shown two zones of staining for isozymes of hexokinase. Genetic analyses 9 indicate that the slower migrating set of bands is encoded by the structural gene Hex2 and that the enzyme is monomeric. Recent linkage analyses and work with B-A translocation stocks (generously supplied by Jack Beckett) have allowed for the placement ofHex2 on the long arm of chromosome 6. Its location relative to other markers on 6L was determined from the analysis of two very large F2 populations generated for other reasons.