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Full-Text Articles in Genetics and Genomics
Arabidopsis Tt8 Orthologue Of Pinto Bean (Phaseolus Vulgaris L.) Regulates Proanthocyanidin Genes And Seed Coat Darkening, Nishat Shayala Islam
Arabidopsis Tt8 Orthologue Of Pinto Bean (Phaseolus Vulgaris L.) Regulates Proanthocyanidin Genes And Seed Coat Darkening, Nishat Shayala Islam
Electronic Thesis and Dissertation Repository
Postharvest darkening of seed coat in pinto bean (Phaseolus vulgaris L.) is an undesirable trait that hinders its market potential. Darkening is more rapid in the cultivars like CDC Pintium than the newly developed slow darkening cultivar 1533-15. A single gene, SLOW DARKENING (Sd), is responsible for the slow darkening in pinto beans and the trait co-segregates with two simple sequence repeat (SSR) markers. The objective of this research is to identify and characterize the Sd gene to understand the slow darkening mechanism in pinto bean seed coat. A search for Sd within the linkage distance from …
Production Of A Candidate Recombinant Protein Vaccine For Mannheimia Haemolytica In Lettuce And Tobacco Chloroplasts, Coby K. Martin
Production Of A Candidate Recombinant Protein Vaccine For Mannheimia Haemolytica In Lettuce And Tobacco Chloroplasts, Coby K. Martin
Electronic Thesis and Dissertation Repository
The cattle industry worldwide is ravaged by bovine respiratory disease (BRD), a bacterial disease caused by Mannheimia haemolytica. Recent efforts to design vaccines against M. haemolytica focus on a virulence factor, leukotoxin, in addition to surface lipoproteins. Plant-based protein production is a safe and inexpensive alternative to traditional methods. Edible vaccines deliver antigens to pharyngeal tissues, which can provide local immunization against M. haemolytica prior to its progression into the lungs. In this project, a chimeric protein containing M. haemolytica antigens was produced in tobacco chloroplasts as a candidate edible vaccine for BRD. Attempts were made to transform lettuce …