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Full-Text Articles in Genetics and Genomics
Raman Multiplexers For Alternative Gene Splicing, Lan Sun, Chenxu Yu, Joseph Irudayaraj
Raman Multiplexers For Alternative Gene Splicing, Lan Sun, Chenxu Yu, Joseph Irudayaraj
Chenxu Yu
Nonfluorescent labels were used for an array-format multiplex detection of alternative splice junctions of breast cancer susceptibility gene 1 (BRCA1) by surface-enhanced Raman scattering (SERS). A four-plex detection scheme using nonfluorescent labels was demonstrated for DNA sequences specific to four BRCA1 alternative splice variants: Δ(11q) (the last 3309 nt deleted from exon 11), Δ(9, 10) (exon 9 and 10 deleted), Δ(5) (exon 5 deleted), and Δ(5q, 6) (the last 22 nt of exon 5 and the entire exon 6 is deleted). This is the first proof-of-concept study to apply SERS-based detection using nonfluorescent labels to investigate alternative gene splicing. Detection …
Surface-Enhanced Raman Scattering Based Nonfluorescent Probe For Multiplex Dna Detection, Lan Sun, Chenxu Yu, Joseph Irudayaraj
Surface-Enhanced Raman Scattering Based Nonfluorescent Probe For Multiplex Dna Detection, Lan Sun, Chenxu Yu, Joseph Irudayaraj
Chenxu Yu
To provide rapid and accurate detection of DNA markers in a straightforward, inexpensive, and multiplex format, an alternative surface-enhanced Raman scattering based probe was designed and fabricated to covalently attach both DNA probing sequence and nonfluorescent Raman tags to the surface of gold nanoparticles (DNA-AuP-RTag). The intensity of Raman signal of the probes could be controlled through the surface coverage of the nonfluorescent Raman tags (RTags). Detection sensitivity of these probes could be optimized by fine-tuning the amount of DNA molecules and RTags on the probes. Long-term stability of the DNA-AuP-RTag probes was found to be good (over 3 months). …