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Articles 1 - 7 of 7
Full-Text Articles in Genetics and Genomics
Resistance To Double-Stranded Rna (Dsrna) In Colorado Potato Beetle (Leptinotarsa Decemlineata Say): Genetic And Molecular Insights, Swati Mishra
Doctoral Dissertations
Double-stranded RNA (dsRNA) insecticides delivered through transgenic and non-transgenic means have been approved for commercialization in the US. As with other insecticides development of insect resistance is expected, necessitating the implementation of an Insecticide Resistance Management (IRM) framework for their long-term use. Laboratory-selected insect resistance strains can provide useful insights for an effective IRM plan. The Colorado potato beetle (CPB) is the target pest for the first commercial dsRNA foliar spray, making it an ideal model to study dsRNA resistance. In this dissertation, we studied the previously described CEAS 300 strain of CPB that exhibits >11,100-fold resistance against V-ATPase subunit …
Assessment Of Genetic Diversity Of The Invasive Callery Pear, Pyrus Calleryana Decne. Using Mitochondrial Microsatellites And Its Molecular Detection Using Lamp, Alina Pokhrel
Masters Theses
Pyrus calleryana Decne. (Callery pear; [PC]) is a popular ornamental tree in the urbanized areas of the United States of America (US), owing to its aesthetical value, with showy white blossoms in early spring and vibrant fall foliage. The tree native to Asia is now becoming one of the most problematic invasive tree species in the eastern US. From its introduction in the early 20th century, PC has been commercially used as rootstocks for propagating fruiting pears, alongside other closely related pear species. Several states are restricting the sale of rootstocks to limit the ongoing spread of PC. As …
A Modular Cloning Toolbox For Chloroplast Metabolic Engineering, Alexander C. Pfotenhauer
A Modular Cloning Toolbox For Chloroplast Metabolic Engineering, Alexander C. Pfotenhauer
Masters Theses
Plant synthetic biology is a rapidly evolving field with new tools constantly emerging to drive innovation. Of particular interest is the application of synthetic biology to chloroplast biotechnology to generate plants capable of producing new metabolites, vaccines, biofuels, and high-value chemicals. Progress made in the assembly of large DNA molecules, composing multiple transcriptional units, has significantly aided in the ability to rapidly construct novel vectors for genetic engineering. In particular, Golden Gate assembly has provided a facile molecular tool for standardized assembly of synthetic genetic elements into larger DNA constructs. In this work a complete modular chloroplast cloning system (MoChlo) …
Quantitative Trait Loci Analysis Of A Ril Soybean Population To Determine Chromosomal Regions Governing Seed Protein, Oil, And Linolenic Acid Content, Ronald E. Moore
Quantitative Trait Loci Analysis Of A Ril Soybean Population To Determine Chromosomal Regions Governing Seed Protein, Oil, And Linolenic Acid Content, Ronald E. Moore
Masters Theses
180 RILs (recombinant inbred lines) segregating for protein, oil, and fatty acids were produced from a cross between TN12-4098 and TN13-4303. These lines were grown across three locations spread horizontally across Tennessee: Research Education Center at Milan (RECM), Highland Rim Research and Education Center (HRREC), and East Tennessee Research and Education Center (ETREC) in 2018 and 2019. 21 quantitative trait loci (QTLs) spanning 7 chromosomes were found using WinQTLCart2.5.
Population Structure Of Late Blight (Phytophthora Infestans) In Colombia And Ecuador And Downy Mildew (Peronospora Farinosa F. Sp. Spinaciae) On Spinach In Arizona And California, Rebecca Marie Lyon
Population Structure Of Late Blight (Phytophthora Infestans) In Colombia And Ecuador And Downy Mildew (Peronospora Farinosa F. Sp. Spinaciae) On Spinach In Arizona And California, Rebecca Marie Lyon
Masters Theses
In this study, Phytophthora infestans and Peronospora farinosa f. sp. spinaciae populations were analyzed using single nucleotide polymorphisms (SNPs). In Ecuador and Colombia, Phytophthora infestans causes significant damage to potato and tomato and the epidemiology is known to be highly clonal. Our objective was to measure population structure within the context of this clonal epidemiology using both synonymous and nonsynonymous markers. Candidate SNP sites were selected by comparing the draft genomes of the Ecuadorian isolates EC1-3527 and EC1-3626. Genotypes were assessed directly from infected tissue using a targeted sequencing approach. A total of 54 polymorphic sites were assessed in 93 …
Characterization Of The Role Of Alpha-Arylphorin In The Heliothis Virescens Midgut Response To Cry1ac Toxin From Bacillus Thuringiensis, Jerreme Jamael Jackson
Characterization Of The Role Of Alpha-Arylphorin In The Heliothis Virescens Midgut Response To Cry1ac Toxin From Bacillus Thuringiensis, Jerreme Jamael Jackson
Doctoral Dissertations
Homeostasis of the intestinal epithelium in Heliothis virescens is mediated by the proliferation and differentiation of multipotent intestinal stem cells (ISCs) that lie adjacent to the basal lamina. In response to extrinsic and intrinsic signals, ISC proliferation and differentiation promotes epithelial growth and regeneration following the loss of integrity. We tested the in vivo effects of the ISC mitogen, a [alpha]-arylphorin, on ISC proliferation and the morphological changes of the midgut during larval development. Additionally, we examined how these changes affected the intestinal epithelium response to Cry1Ac toxin from Bacillus thuringiensis. Histological and in vitro evidence supported two distinct …
Effects Of Leaflet Orientation And Root Morphology On Physiological Traits And Yield In Soybeans., Richard Dewayne Johnson
Effects Of Leaflet Orientation And Root Morphology On Physiological Traits And Yield In Soybeans., Richard Dewayne Johnson
Doctoral Dissertations
Drought is the most important abiotic stress adversely affecting soybean (Glycine max (L.) Merr.) yield. Leaflet orientation has been shown to reduce leaflet temperatures and transpiration while root morphology has been related to slower wilting phenotypes. The objective of this study was to investigate effects of leaflet orientation and rooting morphology on whole plant transpiration, yield, water use efficiency, and other physiological traits in soybeans using grafting techniques, population lines, near-isogenic lines, and restrained leaf canopy experiments. Experiments were conducted in Knoxville, TN with additional yield trial plots at Springfield, Spring Hill, and Milan, TN. Data were collected on …