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Characterization Of Aspen Isoprene Synthase, An Enzyme Responsible For Leaf Isoprene Emission To The Atmosphere, Gary M. Silver, Ray Fall
Characterization Of Aspen Isoprene Synthase, An Enzyme Responsible For Leaf Isoprene Emission To The Atmosphere, Gary M. Silver, Ray Fall
Aspen Bibliography
Isoprene (2-methyl-1,3-butadiene) is a volatile hydrocarbon emitted from many plant species to the atmosphere, where it plays an important role in atmospheric chemistry. An enzyme extracted from aspen (Populus tremuloides) leaves was previously found to catalyze the Mg2+-dependent elimination of pyrophosphate from dimethylallyl diphosphate (DMAPP) to form isoprene (Silver, G. M., and Fall, R.(1991) Plant Physiol. 97, 1588-1591). This enzyme, isoprene synthase, has now been purified 4000-fold to near homogeneity. The enzyme had a native molecular mass of 98-137 kDa and isoelectric point of 4.7 and contained 58- and 62-kDa subunits, implying that it is a heterodimer. …