Forest Sciences Commons^™

Oystershell Scale (Hemiptera: Diaspididae) Population Growth, Spread, And Phenology On Aspen In Arizona, Usa, Connor D. Crouch, Richard W. Hofstetter, Amanda M. Grady, Nylah N.S. Edwards, Kristen M. Waring

Aspen Bibliography

Oystershell scale (OSS; Lepidosaphes ulmi L.) is an invasive insect that threatens sustainability of aspen (Populus tremuloides Michx.) in the southwestern United States. OSS invasions have created challenges for land managers tasked with maintaining healthy aspen ecosystems for the ecological, economic, and aesthetic benefits they provide. Active management is required to suppress OSS populations and mitigate damage to aspen ecosystems, but before management strategies can be implemented, critical knowledge gaps about OSS biology and ecology must be filled. This study sought to fill these gaps by addressing 3 questions: (i) What is the short-term rate of aspen mortality in …

Pile Burning After Conifer Removal From Aspen Stands Affects Tree Mortality, Regeneration, And Understory Recovery, John-Pascal Berrill, Christa M. Dagley, Yoon G. Kim, J. Morgan Varner

Aspen Bibliography

Quaking aspen (Populus tremuloides Michx.) stands throughout the western United States provide valuable ecosystem services but can be lost via succession from aspen to conifer. Forest managers are cutting conifers, but disposal of cut wood can be challenging in remote or sensitive areas. Piling and burning is being tested within aspen stands but ecosystem responses to this treatment are understudied. We assessed aspen tree mortality, tree regeneration, and understory vegetation after forest restoration thinning followed by pile burning in seven aspen-conifer stands around Lake Tahoe, California and Nevada, USA. Pile burning was conducted after cut wood had dried (1.5–7.5 …

Aspen And Spruce Densities Affect Tree Size, Future Stand Volume, And Aboveground Carbon Following Precommercial Thinning, Philip G. Comeau, Mike Bokalo

Aspen Bibliography

Data collected over a 30-year period from an experiment replicated across 21 locations in western Canada are used to explore the effects of precommercial thinning of trembling aspen to a range of densities in combination with three initial white spruce densities on tree growth and stand dynamics. Increasing differentiation amongst the 15 treatments was observed with age after thinning for both spruce and aspen responses. Spruce height and diameter declined with increasing aspen density. At age 10 spruce diameter with no aspen was 1.5× that of spruce in unthinned while it was 2.6× that of spruce in unthinned at age …

Tracing The Maternal Line In Glacial-Interglacial Migrations Of Populus Tremuloides: Finding Trees For Future Sustainable Forests By Searching In The Past, Luke R. Tembrock, Frida A. Zink, Guozhe Zhang, Andrea Schuhmann, Cuihua Gu, Zhiqiang Wu

Aspen Bibliography

Maintaining and planting sustainable forests is fundamental in perpetuating the essential functions of these ecosystems. A central aspect of managing forests for future resilience is the consideration of past migration and evolution of trees using genetic and genomic data to ensure that functionally appropriate diversity is conserved and utilized. In our study, we generated and compared genetic and genomic data from the plastome to better understand phylogeography and molecular evolution in the tree species Populus tremuloides (aspen). With these analyses, we found evidence of divergence and migration between northern and southern sites. Additionally, evidence of deep incomplete plastome sorting across …

Investigating Birds As Dispersal Vectors Of Litylenchus Crenatae Subsp. Mccannii (Anguinidae), The Nematode Associated With Beech Leaf Disease, Spencer Rock Parkinson

Graduate Theses, Dissertations, and Problem Reports

Beech leaf disease (BLD) is an emerging forest pathogen primarily affecting American beech (Fagus grandifolia, Ehrh.) in North America and has been attributed to tree mortality of sapling sized trees within five to seven years of infection. Symptoms typically occur in regenerating American beech thickets sprouting from roots of trees killed by beech bark disease. Scientists first observed BLD in Ohio in 2012 and currently has spread to 15 states in the USA and one Canadian province. The nematode Litylenchus crenatae subsp. mccannii (Lcm) is highly associated with BLD symptoms, interveinal chlorosis and defoliation of leaves, and is currently …

Exploring Soil Microbial Dynamics In Southern Appalachian Forests: A Systems Biology Approach To Prescribed Fire Impacts, Saad Abd Ar Rafie

Doctoral Dissertations

Prescribed fires in Southern Appalachian forests are vital in ecosystem management and wildfire risk mitigation. However, understanding the intricate dynamics between these fires, soil microbial communities, and overall ecosystem health remains challenging. This dissertation addresses this knowledge gap by exploring selected aspects of this complex relationship across three interconnected chapters.

The first chapter investigates the immediate effects of prescribed fires on soil microbial communities. It reveals subtle shifts in porewater chemistry and significant increases in microbial species richness. These findings offer valuable insights into the interplay between soil properties and microbial responses during the early stages following a prescribed fire. …

Characterization Of Lignin Structural Variability And The Associated Application In Genome Wide Association Studies, Nathan D. Bryant

Doctoral Dissertations

Poplar (Populus sp.) is a promising biofuel feedstock due to advantageous features such as fast growth, the ability to grow on marginal land, and relatively low lignin content. However, there is tremendous variability associated with the composition of biomass. Understanding this variability, especially in lignin, is crucial to developing and implementing financially viable, integrated biorefineries. Although lignin is typically described as being comprised of three primary monolignols (syringyl, guaiacyl, p-hydroxyphenyl), it is a highly irregular biopolymer that can incorporate non-canonical monolignols. It is also connected by a variety of interunit linkages, adding to its complexity. Secondary cell wall …

The Discovery Of A Novel Bacteria From A Large Co-Assembly Of Metagenomes, Matthew Finkelberg

Masters Theses

In the summer of 2022, a co-assembly of metagenome was created using the microbes found at Barres Woods in Harvard Forest. 14 samples were taken, and sample was split into the organic and mineral layer, which totals 28 Bulk MAGs. Within this Co-assembly, 4 different genomes were found which were designated with the phylum of FCPU426. Three of which were considered medium quality and one being assigned high quality. The novel phyla first appeared in NCBI and GTDB databases in June 2018. The name FCPU426 dates to 2010 and was named based on the 16s amplicon sequencing.

The novel phylum …

Forest Composition Change And Biophysical Climate Feedbacks Across Boreal North America, Richard Massey, Brendan M. Rogers, Logan T. Berner, Sol Cooperdock, Michelle C. Mack, Xanthe J. Walker, Scott J. Goetz

Aspen Bibliography

Deciduous tree cover is expected to increase in North American boreal forests with climate warming and wildfire. This shift in composition has the potential to generate biophysical cooling via increased land surface albedo. Here we use Landsat-derived maps of continuous tree canopy cover and deciduous fractional composition to assess albedo change over recent decades. We find, on average, a small net decrease in deciduous fraction from 2000 to 2015 across boreal North America and from 1992 to 2015 across Canada, despite extensive fire disturbance that locally increased deciduous vegetation. We further find near-neutral net biophysical change in radiative forcing associated …

Genomic And Transcriptomic Analyses Reveal Polygenic Architecture For Ecologically Important Traits In Aspen (Populus Tremuloides Michx.), Jennifer F. L. Riehl, Christopher T. Cole, Clay J. Marrow, Hilary L. Barker, Carolina Bernhardsson, Kennedy Rubert-Nason, Pär K. Ingvarsson, Richard L. Lindroth

Aspen Bibliography

Intraspecific genetic variations in foundation species such as aspen (Populus tremuloides Michx.) shapes their impact on forest structure and function. Identifying genes underlying ecologically important traits is key to understanding that impact. Previous studies, using single-locus genome-wide association (GWA) analyses to identify candidate genes, have identified fewer genes than anticipated for highly heritable quantitative traits. Mounting evidence suggests that polygenic control of quantitative traits is largely responsible for this "missing heritability" phenomenon. Our research characterized the genetic architecture of 30 ecologically important traits using a common garden of aspect through genomic and transcriptomic analyses. A multilocus association model revealed …

Bison Alter The Northern Yellowstone Ecosystem By Breaking Aspen Saplings, Luke E. Painter, Robert L. Beschta, William J. Ripple

Aspen Bibliography

The American bison (Bison bison) is a species that strongly interacts with its environment, yet the effects of this large herbivore on quaking aspen (Populus tremuloides) have received little study. We documented bison breaking the stems of aspen saplings (young aspen > 2 m tall and ≤ 5 cm in diameter at breast height) and examined the extent of this effect in northern Yellowstone National Park (YNP). Low densities of Rocky Mountain elk (Cervus canadensis) after about 2004 created conditions conducive for new aspen recruitment in YNP's northern ungulate winter range (northern range). We sampled …

Gene Expression Effects On Productivity And Stress Tolerance In Polyclonal Plantings Of Populus Deltoides, Macy Gosselaar

Theses and Dissertations

Polyclonal plantings of Populus deltoides are expected to display increased site resource use, productivity, and tolerance to stress through plasticity changes leading to niche differentiation (i.e changes to crown/canopy structures). In the present study, P. deltoides Clones S7C8, 110412, and polyclonal plots were tested for differentially expressed genes and enriched biological pathways between planting schemes. Transcriptomic analysis of leaves revealed upregulation of an active growth gene and gene family members that play important roles in plant stress and stress tolerance in polyclonal plantings. A gene associated with oxidative stress was upregulated in polyclonal plantings across all treatments. Secondary metabolic pathways …

The Impact Of Soil Disturbance On Soil Bacterial Community Composition, Marie A. Rodriguez, Mark Peach, Timothy D. Trott

Research in Biology

Soil bacterial communities are an important part of terrestrial ecosystems due to their roles in biogeochemical cycling processes. Consequently, understanding how soil disturbance affects the soil bacterial diversity is vital to understanding the entire ecosystem. In this study we examined the effects of soil disturbance (by mining) on the soil bacterial community composition from three sites on Bauxite Ridge in Southeast Tennessee compared to three undisturbed sites in a nearby location. The soil bacterial community was analyzed by 16S rDNA amplicon sequencing of total DNA extracted from the soil samples collected from each of the six sites. Characterization of the …

Variable Synchrony In Insect Outbreak Cycling Across A Forest Landscape Gradient: Multi-Scale Evidence From Trembling Aspen In Alberta, Barry J. Cooke, Jens Roland

Aspen Bibliography

Using multi-scale trembling aspen tree-ring width data from Alberta, we show that scaling has a profound influence on dendroecological inferencing. At all scales of sampling, there is a significant climatological signal whose strength is nevertheless superseded by the pervasive effect of insect herbivory. At the smallest spatial scale, 20 km x 20 km, we demonstrate a quasi-periodic pattern of sharp growth reductions and the existence of negative spatial correlations among successive outbreaks. At the intermediate spatial scale of 20 km x 80 km, we show that the period 1930-1963 was marked by extremely low correlations in aspen ring widths, with …

Du Undergraduate Showcase: Research, Scholarship, And Creative Works, Caitlyn Aldersea, Justin Bravo, Sam Allen, Anna Block, Connor Block, Emma Buechler, Maria De Los Angeles Bustillos, Arianna Carlson, William Christensen, Olivia Kachulis, Noah Craver, Kate Dillon, Muskan Fatima, Angel Fernandes, Emma Finch, Colleen Cassidy, Amy Fishman, Andrea Francis, Stacia Fritz, Simran Gill, Emma Gries, Rylie Hansen, Shannon Powers, Jacqueline Martinez, Zachary Harker, Ashley Hasty, Mykaela Tanino-Springsteen, Kathleen Hopps, Adelaide Kerenick, Colin Kleckner, Ci Koehring, Elijah Kruger, Braden Krumholz, Maddie Leake, Lyneé Alves, Seraphina Loukas, Yatzari Lozano Vazquez, Haley Maki, Emily Martinez, Sierra Mckinney, Mykaela Tanino-Springsteen, Audrey Mitchell, Kipling Newman, Audrey Ng, Megan Lucyshyn, Andrew Nguyen, Stevie Ostman, Casandra Pearson, Alexandra Penney, Julia Gielczynski, Tyler Ball, Anna Rini, Christina Rorres, Simon Ruland, Helayna Schafer, Emma Sellers, Sarah Schuller, Claire Shaver, Kevin Summers, Isabella Shaw, Madison Sinar, Claudia Pena, Apshara Siwakoti, Carter Sorensen, Madi Sousa, Anna Sparling, Alexandra Revier, Brandon Thierry, Dylan Tyree, Maggie Williams, Lauren Wols

DU Undergraduate Research Journal Archive

DU Undergraduate Showcase: Research, Scholarship, and Creative Works

Remote Sensing In Mapping Biodiversity – A Case Study Of Epiphytic Lichen Communities, Ida Palmroos, Veera Norros, Sarita Keski-Saari, Janne Mäyrä, Topi Tanhuanpää, Sonja Kivinen, Juha Pykälä, Peter Kullberg, Timo Kumpula, Petteri Vihervaara

Aspen Bibliography

In boreal forests, European aspen (Populus tremula L.) is a keystone species that hosts a variety of accompanying species including epiphytic lichens. Forest management actions have led to a decrease in aspen abundance and subsequent loss of suitable habitats of epiphytic lichens. In this study, we evaluate the environmental responses of epiphytic lichen species richness and community composition on aspen, focusing on the potential of remote sensing by combined hyperspectral imaging and airborne laser scanning to identify suitable habitats for epiphytic lichens. We measured different substrate and habitat parameters in the field (e.g., aspen diameter and bark pH) …

Genetic Markers And Tree Properties Predicting Wood Biorefining Potential In Aspen (Populus Tremula) Bioenergy Feedstock, Sacha Escamez, Kathryn M. Robinson, Mikko Luomaranta, Madhavi Latha Gandla, Niklas Mähler, Zakiya Yassin, Thomas Grahn, Gerhard Scheepers, Lars-Göran Stener, Stefan Jansson, Leif J. Jönsson, Nathaniel R. Street, Hannele Tuominen

Aspen Bibliography

Background Wood represents the majority of the biomass on land and constitutes a renewable source of biofuels and other bioproducts. However, wood is recalcitrant to bioconversion, raising a need for feedstock improvement in production of, for instance, biofuels. We investigated the properties of wood that affect bioconversion, as well as the underlying genetics, to help identify superior tree feedstocks for biorefining.

Results We recorded 65 wood-related and growth traits in a population of 113 natural aspen genotypes from Sweden (https://doi.org/10.5061/dryad.gtht76hrd). These traits included three growth and field performance traits, 20 traits for wood chemical composition, 17 traits for wood anatomy …

Less Fuel For The Next Fire? Short-Interval Fire Delays Forest Recovery And Interacting Drivers Amplify Effects, Kristin H. Braziunas, Nathan G. Kiel, Monica G. Turner

Aspen Bibliography

As 21st-century climate and disturbance dynamics depart from historic baselines, ecosystem resilience is uncertain. Multiple drivers are changing simultaneously, and interactions among drivers could amplify ecosystem vulnerability to change. Subalpine forests in Greater Yellowstone (Northern Rocky Mountains, USA) were historically resilient to infrequent (100–300 year), severe fire. We sampled paired short-interval (<30-year) and long-interval (>125-year) post-fire plots most recently burned between 1988 and 2018 to address two questions: (1) How do short-interval fire, climate, topography, and distance to unburned live forest edge interact to affect post-fire forest regeneration? (2) How do forest biomass and fuels vary following short-interval versus long-interval severe fires? …

Regeneration Strategies And Forest Resilience To Changing Fire Regimes: Insights From A Goldilocks Model, Tanjona Ramiadantsoa, Zak Ratajczak, Monica G. Turner

Aspen Bibliography

Disturbances are ubiquitous in ecological systems, and species have evolved a range of strategies to resist or rebound following disturbance. Understanding how the presence and complementarity of regeneration traits will affect community responses to disturbance is increasingly urgent as disturbance regimes shift beyond their historical ranges of variability. We define "disturbance niche" as a species' fitness across a range of disturbance sizes and frequencies that can reflect the fundamental or realized niche, that is, whether the species occurs alone or with other species. We developed a model of intermediate complexity (i.e., a Goldilocks model) to infer the disturbance niche. We …

Revisiting Trophic Cascades And Aspen Recovery In Northern Yellowstone, Robert L. Beschta, Luke E. Painter, William J. Ripple

Aspen Bibliography

We revisit the nature and extent of trophic cascades and quaking aspen (Populus tremuloides) recovery in the northern range of Yellowstone National Park (YNP), where studies have reported on Rocky Mountain elk (Cervus canadensis) browsing and young aspen heights following the St. John, 1995-96 reintroduction of gray wolves (Canis lupus). A recent study by Brice et al. (2021) expressed concerns about methodologies employed in earlier aspen studies and that results from those studies exaggerated the extent to which a trophic cascade has benefitted aspen, concerns such as: (a) the selection of aspen stands, (b) …

Bison Influences On Composition And Diversity Of Riparian Plant Communities In Yellowstone National Park, J. Boone Kauffman, Dian Lyn Cummings, Cimarron Kauffman, Robert L. Beschta, Jeremy Brooks, Keeley Macneill, William J. Ripple

Aspen Bibliography

Riparian zones are among the most biologically diverse ecosystems in the Intermountain West, USA, and provide valuable ecosystem services, including high rates of biotic productivity, nutrient processing, and carbon storage. Thus, their sustainability is a high priority for land managers. Large ungulates affect composition and structure of riparian/stream ecosystems through herbivory and physical effects, via trailing and trampling. Bison (Bison bison) in Yellowstone National Park (YNP) have been characterized as "ecosystem engineers" because of their demonstrated effects on phenology, aboveground productivity of grasses, and woody vegetation structure. Bison have greatly increased in numbers during the last two decades …

Assessment Of Carbon Productivity Dynamics In Aspen Stands Under Climate Change Based On Forest Inventories In Central Siberia, Andrey Andreevich Vais, Valentina Valerievna Popova, Alina Andreevna Andronova, Viktor Nikolaevich Nemich, Artem Gennadievich Nepovinnykh, Pavel Vladimirovich Mikhaylov

Aspen Bibliography

The aim of the present research was to study the dynamics of growth and conditions of aspen stands under climate change, according to different periods of forest inventory. The study was conducted in modal aspen forests growing in the subtaiga/forest steppe region of Central Siberia. Aspen forests grow intensively at young age, which allows them to realize maximum carbon sequestration potential. The research was based on forest inventory data from 1972, 1982, 2002, and 2021 (the study was conducted on a limited territory). There was a steady increase in temperatures in the growing season from 1982 to 2002. The amount …

Sequencing Antisense Transcriptome Of Populus Through Antisense/Sense Transcript Pair Enrichment, Emma V. Burke

Dissertations, Master's Theses and Master's Reports

Past transcriptome research on plants focused primarily on protein coding genes, and only recently researchers began looking into the non-protein coding regions that may play significant roles in gene regulation. Antisense RNA transcripts that are found naturally in the cell share complementary sequence with sense transcripts and have been shown to regulate expression of their sense counterparts. Since antisense RNA has been largely under-studied and difficult to sequence because of their low relative abundance, new methods are needed to target antisense RNA for efficient genome-wide profiling. To address this gap in methods to efficiently and cost effectively enrich antisense RNA …

White-Tailed Deer In A Winter-Wonderland: Long-Term Deer Yard Use And Methodological Considerations For Ungulate Fecal Dna Metabarcoding, Melanie A. Ottino

Dissertations, Master's Theses and Master's Reports

Northern white-tailed deer (Odocoileus virginianus) mitigate the increased energetic costs of severe winter conditions through obligate migration to densely congregated areas with abundant conifer cover, a behavior referred to as yarding. “Deer yards” in the Western Upper Peninsula (UP) of Michigan are principally located within the reduced snowpack and increased ambient temperature microclimates of densely canopied eastern hemlock (Tsuga canadensis) stands, but specific drivers of long-term site fidelity and utilization are largely uncharacterized. As an important game species of high economic and cultural value and a keystone herbivore with critical impact of plant community composition and structure, identifying winter yarding …

Big Meadows Mountain Meadow & Aspen Restoration Project 2017 – 2023 Summary Report, Scott River Watershed Council

Aspen Bibliography

Big Meadows Mountain Meadow and Aspen Restoration Project is designed to enhance aspen and mountain meadow habitats at Big Meadows, a property that is privately owned by Ecotrust Forest Management (EFM). EFM owns approximately 40,000 acres in the Klamath Mountains of Siskiyou County, known as the Scott River Headwaters Property. The Scott River Headwaters Property Management Plan, developed by EFM, specifically identifies and prioritizes the restoration of aspen woodlands. As such, EFM and Scott River Watershed Council (SRWC) partnered with the goal of improving these critical habitats. The Big Meadows complex is one of the largest mountain meadow systems within …

Tree Biomass – A Fragile Carbon Storage In Old-Growth Birch And Aspen Stands In Hemiboreal Latvia, Laura Ķēniņa, Didzis Elferts, Ieva Jaunslaviete, Endijs Bāders, Guntars Šņepsts, Āris Jansons

Aspen Bibliography

Birch (Betula pendula Roth, Betula pubescens Ehrh.) and European aspen (Populus tremula L.) stands dominate the deciduous forests of Northern Europe. Due to increasing forest protections, more deciduous stands will reach the old-growth stage. Thus, data on the carbon storage potential in such areas are essential. We aimed to establish a benchmark for carbon stocks of the main carbon pools in old-growth deciduous hemiboreal stands. Carbon pools were calculated from measurements in forty old-growth (104–148 years-old) deciduous stands in forests on fertile mineral soil. The carbon stock in these stands is distributed across tree biomass (~ 60%), mineral soil (~ …

Aspen Leaves As A "Chemical Landscape" For Fungal Endophyte Diversity - Can Nitrogen And Herbivory Shape The Community Composition In Controlled Conditions?, Johanna Witzell, Vicki Huizu Guo Decker, Marta Agostinelli, Carmen Romeralo Tapia, Michelle Cleary, Benedicte Riber Albrectsen

Aspen Bibliography

The endophytic microbiome may influence the ecological performance of plants, including forest trees. Various abiotic and biotic factors may shape the endophyte communities directly but also indirectly, by modifying the quality of host plants as a substrate. We hypothesized that potentially antifungal or fungistatic condensed tannins (CTs) would determine the quality of aspen (Populus tremula) leaves as a substrate for endophytic fungi. By subjecting the plants to nitrogen fertilization (N) or herbivory (H; leaf beetles) we aimed to change the internal “chemical landscape”, especially the CT levels, in aspen leaves. We expected that this would lead to changes in the …

Disease Resistance And Productivity In Genetically Improved Loblolly Pine: Results From A Resistance Screening Trial And A Midrotation Comparison Of Genetic Improvement Levels, Stephen W. Goodfellow

Theses and Dissertations

Decades of tree improvement has resulted in genetic gains in loblolly pine productivity, form, and resistance to fusiform rust. The goal of this study was to advance the understanding and applied use of genetic improvement by analyzing inter- and intra-provenances hybrids’ rust resistance and evaluating midrotation performance of varying levels of genetically improved stock types. The first study compares 16 seedlots at the USDA Resistance Screening Center and evaluates rust resistance of controlled-pollinated inter- and intra-provenances crosses, and openpollinated seedlots from three provenances: Western Gulf, Atlantic Coastal, and Interior Piedmont. Post inoculation, one Coastal OP seedlot was resistant and ten …

Ungulate Herbivores As Drivers Of Aspen Recruitment And Understory Composition Throughout Arid Montane Landscapes, Elizabeth S. Reikowski, Tyler Refsland, J. Hall Cushman

Aspen Bibliography

Herbivory by wild and domestic ungulates can influence tree recruitment and understory forest communities throughout the world. Herbivore-driven declines in tree recruitment have been observed for quaking aspen (Populus tremuloides), a foundation species whose health and management is recognized as a critical priority throughout much of its range. Livestock fencing is commonly used to promote aspen regeneration, but its effectiveness is rarely assessed, especially across large spatial scales. Using a livestock-reduction experiment, we evaluated the effects of ungulate herbivory on aspen in the Great Basin and southern Cascades, an expansive and environmentally heterogeneous region where aspen faces the …

Coping With Environmental Constraints: Geographically Divergent Adaptive Evolution And Germination Plasticity In The Transcontinental Populus Tremuloides, Roos Goessen, Nathalie Isabel, Christian Wehenkel, Nathalie Pavy, Lisa Tischenko, Lyne Touchette, Isabelle Giguère, Marie-Claude Gros-Louis, Jérôme Laroche, Brian Boyle, Raju Soolanayakanahally, Karen E. Mock, Javier Hernández-Velasco, Sergio Leonel Simental-Rodriguez, Jean Bousquet, Ilga M. Porth

Aspen Bibliography

Societal Impact Statement

Syntheses clearly show that global warming is affecting ecosystems and biodiversity around the world. New methods and measures are needed to predict the climate resilience of plant species critical to ecosystem stability, to improve ecological management and to support habitat restoration and human well-being. Widespread keystone species such as aspen are important targets in the study of resilience to future climate conditions because they play a crucial role in maintaining various ecosystem functions and may contain genetic material with untapped adaptive potential. Here, we present a new framework in support of climate-resilient revegetation based on comprehensively understood …