Forest Sciences Commons^™

Quantification And Characterization Of Net Precipitation Bacterial Flux From A Subtropical Epiphyte-Laden Oak Forest, Preston Pound

Electronic Theses and Dissertations

Transport pathways of microbes between ecosystem spheres (atmosphere, phyllosphere, and pedosphere) represent major fluxes in nutrient cycles and have the potential to significantly affect microbially-mediated biogeochemical processes. We quantified a previously unexamined microbial flux from the phyllosphere to the pedosphere during rainfall via throughfall (rainfall dripping from the canopy and through gaps) and stemflow (rainwater funneled down the stem). Bacterial concentrations were quantified using flow cytometry and validated with quantitative Polymerase Chain Reaction (qPCR) assays for samples from a Quercus virginiana (oak)- forest with heavy epiphyte cover (Tillandsia usneoides, Spanish moss) in coastal Georgia (Southeastern USA). Bacteria concentrations …