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Improved Keratinase Production For Feather Degradation By Bacillus Licheniformis Zjuel31410 In Submerged Cultivation, Hui Ni, Qi-He Chen, Feng Chen, Ming-Liang Fu, Ya-Chen Dong, Hui-Nong Cai
Improved Keratinase Production For Feather Degradation By Bacillus Licheniformis Zjuel31410 In Submerged Cultivation, Hui Ni, Qi-He Chen, Feng Chen, Ming-Liang Fu, Ya-Chen Dong, Hui-Nong Cai
Feng Chen
Optimal medium was used to improve the production of keratinase by Bacillus licheniformis ZJUEL31410, which has a promising application in the transformation of feather into soluble protein. The results of single factor design revealed that the concentration of feather at 20 g/l and the initial pH at value 8 was the best for the production of keratinase and the degradation of feather. Ammonia salt and nitrate salt strongly restricted the production of keratinase and the degradation of feather. Result of Box-Behnken design (BBD) experiment which was used to optimize concentrations of glucose, corn steep flour and K2HPO4 for further improvement …