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Full-Text Articles in Food Science
Establishment Of Double Antibody Sandwich Elisa For Peanut Allergenic Protein Ara H 1, Wang Yao, Chen Xi, Wu Han-Liang, Wei Xing, Zhang Guo-Qing, Zhang Shu-Xia, Liu Sheng-Nan
Establishment Of Double Antibody Sandwich Elisa For Peanut Allergenic Protein Ara H 1, Wang Yao, Chen Xi, Wu Han-Liang, Wei Xing, Zhang Guo-Qing, Zhang Shu-Xia, Liu Sheng-Nan
Food and Machinery
In order to establish a double antibody sandwich ELISA method for the rapid, sensitive and specific detection of peanut allergenic protein Ara h 1. Taking Ara h 1 murine monoclonal antibody (mAb) as capture antibody, and Ara h 1 rabbit polyclonal antibody (pAb) as detection antibody, the checkerboard method was used to optimize the antibody working concentration to establish the method, and the detection characteristics were identified, including sensitivity, specificity, accuracy and stability of the method. Results: The optimal working concentrations of mAb and pAb were 1∶10 000 and 1∶8 000 dilution, respectively. The linear range of the ELISA standard …
Research Progress Of Improved Enzyme-Linked Immunosorbent Assay Based On Nanomaterials, Zhang Guang-Yin, Lu Dai, Deng Fang-Ming, Zhao Qian, Shi Xing-Bo
Research Progress Of Improved Enzyme-Linked Immunosorbent Assay Based On Nanomaterials, Zhang Guang-Yin, Lu Dai, Deng Fang-Ming, Zhao Qian, Shi Xing-Bo
Food and Machinery
The recent development of improved ELISA was summarized. Nanomaterials as a substitute for traditional biological enzymes and signal substrates for colorimetry, fluorescence, electrochemistry, photothermal effects, surface enhanced Raman scattering, and chemiluminescence were also reviewed. Finally, the development direction and challenges of the improved ELISA were analyzed, and its application prospect was prospected.