Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Entomology
Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang
Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang
LSU Doctoral Dissertations
The sugarcane borer, Diatraea saccharalis, is a major target pest of transgenic corn expressing Bacillus thuringiensis (Bt) proteins in many areas of the U.S. mid-southern region. A Cry1Ab-resistant (Cry1Ab-RR) strain of D. saccharalis has been developed from a single two-parent family-line. To examine the molecular mechanisms of the Cry1Ab resistance in this insect strain, cDNAs of five types of potential candidate genes related to Bt resistance were sequenced using reverse transcriptase polymerase chain reaction (RT-PCR) and 5’ rapid amplification of cDNA end (5’ RACE). The Bt resistance candidate genes examined included three trypsins (DsTRYs), three chymotrypsins (DsCHYs), three aminopeptidases N …
Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang
Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang
LSU Doctoral Dissertations
The sugarcane borer, Diatraea saccharalis, is a major target pest of transgenic corn expressing Bacillus thuringiensis (Bt) proteins in many areas of the U.S. mid-southern region. A Cry1Ab-resistant (Cry1Ab-RR) strain of D. saccharalis has been developed from a single two-parent family-line. To examine the molecular mechanisms of the Cry1Ab resistance in this insect strain, cDNAs of five types of potential candidate genes related to Bt resistance were sequenced using reverse transcriptase polymerase chain reaction (RT-PCR) and 5’ rapid amplification of cDNA end (5’ RACE). The Bt resistance candidate genes examined included three trypsins (DsTRYs), three chymotrypsins (DsCHYs), three aminopeptidases N …