Entomology Commons^™

Analysis Of Gene Expression During Embryonic Development In Mulberry Silkworm Bombyx Mori, Amit Singh, K. P. Gopinathan

Biology Faculty Publications

We have developed a method for dechorionation and devitellinization of the silkworm eggs without damage, to facilitate the analysis of gene expression during embryonic development of Bombyx mori. Making use of antibodies available from heterologous systems, the spatio-temporal expression patterns of peroxidase and proliferating cell nuclear antigen have been directly visualized in whole mount embryos at various stages of development without the need for generating transformed ·lines carrying specific reporter constructs. The B. mori system, previously unamenable for such studies, could thus serve as an attractive model for molecular analysis of insect development.

The attention lavished on Drosophila melanogaster as …

Host Plant Phenology And Dispersal By A Montane Butterfly: Causes And Consequences Of Uphill Movement, Merrill A. Peterson

Biology Faculty and Staff Publications

In the Wenatchee Mountains of central Washington State, populations of the lycaenid butterfly Euphilotes enoptes occur patchily with their sole host plant, Eriogonum compositum (Polygonaceae). Nearly all courtship and adult feeding occur on the inflorescences of this long-lived perennial. Furthermore, because females oviposit on inflorescences and larvae feed only on flowers and developing seeds, the window of opportunity for exploiting this resource is narrow. I demonstrated that inflorescence phenology varied according to the aspect and elevation of plant patches, and butterflies were most likely to occur in patches nearing full bloom. A mark–release–recapture study revealed that individual butterflies can disperse …

Problems With The Interpretation Of Mark-Release-Recapture Data In Subterranean Termites (Isoptera: Rhinotermitidae), Anthony D. Curtis, Deborah A. Waller

Biological Sciences Faculty Publications

The Lincoln index (Peterson method) is frequently used to estimate animal population size in mark-release-recapture studies. We tested the accuracy of this method to estimate termite colony size using logs infested with termites that were maintained in the laboratory. Termites were fed paper towels dyed either with 0.05% or 0.1% (w/w) of the dye marker Nile blue and released into their host logs in the laboratory. Following recapture a week later, estimates of termite population size for termites dyed with 0.05% Nile blue, and were approximately 3 times greater for termites dyed with 0.1% Nile blue. Concentrations of 0.1% Nile …