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University of Nebraska - Lincoln

Department of Entomology: Faculty Publications

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Full-Text Articles in Entomology

Using Genetic Comparisons Of Populations From Arizona, Mexico, And Texas To Investigate Fall Armyworm Migration In The American Southwest, Rodney N. Nagoshi, Ashley E. Tessnow, Yves Carrière, Jeff Bradshaw Dr., Kyle Harrington, Gregory A. Sword, Robert L. Meagher Nov 2023

Using Genetic Comparisons Of Populations From Arizona, Mexico, And Texas To Investigate Fall Armyworm Migration In The American Southwest, Rodney N. Nagoshi, Ashley E. Tessnow, Yves Carrière, Jeff Bradshaw Dr., Kyle Harrington, Gregory A. Sword, Robert L. Meagher

Department of Entomology: Faculty Publications

Fall armyworm (FAW) is a global agricultural pest, causing substantial economic losses in corn and many other crops. Complicating efforts to control this pest is its capacity for long distance flights, which has been described in greatest detail for the central and eastern sections of the United States. FAW infestations are also routinely found in agricultural areas in southern Arizona, which lie beyond the western limits of the mapped migratory pathways. Climate suitability analysis found that the affected Arizona locations cannot support permanent FAW populations, indicating that these FAW most likely arise from annual migrations. A better understanding of this …


Re‑Using Food Resources From Failed Honey Bee (Apis Mellifera L.) Colonies And Their Impact On Colony Queen Rearing Capacity, Rogan Tokach, Autumn H. Smart, Judy Wu-Smart Oct 2023

Re‑Using Food Resources From Failed Honey Bee (Apis Mellifera L.) Colonies And Their Impact On Colony Queen Rearing Capacity, Rogan Tokach, Autumn H. Smart, Judy Wu-Smart

Department of Entomology: Faculty Publications

For over a decade, beekeepers have experienced high losses of honey bee (Apis mellifera L.) colonies due to a variety of stressors including pesticide exposure. Some of these chemical stressors may residually remain in the colony comb and food resources (pollen and nectar) of failed colonies and be later re-used by beekeepers when splitting and building back new colonies. The practice of re-using comb from previously perished colonies (termed “deadout”) is common in beekeeping practice, but its role in affecting colony health is not well understood. Here, we evaluate the impact of reused, pesticide-contaminated “deadout” combs on colony function …


Editorial. In: Chaboo Cs, Schmitt M (Eds) Research On Chrysomelidae 9, Caroline S. Chaboo, Michael Schmitt Aug 2023

Editorial. In: Chaboo Cs, Schmitt M (Eds) Research On Chrysomelidae 9, Caroline S. Chaboo, Michael Schmitt

Department of Entomology: Faculty Publications

This is volume 9 in the series ‘Research on Chrysomelidae (RoC-9)’. This started from RoC-1 in 2008, named as a series to reflect the dream of Pierre Jolivet for a long tradition into the future. This dream was built on previous special volumes of Chrysomelidae research that emerged largely through Jolivet’s efforts since 1988 (‘Biology of Chrysomelidae’) and from various international symposia. For example, ‘Special Topics in Leaf Beetle Biology’, edited by David Furth, emerged from the International Congress of Entomology held in Iguassu, Brazil in 2000.


Temporal Transcriptomic Profiling Elucidates Sorghum Defense Mechanisms Against Sugarcane Aphids, Heena Puri, Sajjan Grover, Lise Pingault, Scott E. Sattler, Joe Louis Aug 2023

Temporal Transcriptomic Profiling Elucidates Sorghum Defense Mechanisms Against Sugarcane Aphids, Heena Puri, Sajjan Grover, Lise Pingault, Scott E. Sattler, Joe Louis

Department of Entomology: Faculty Publications

Background The sugarcane aphid (SCA; Melanaphis sacchari) has emerged as a key pest on sorghum in the United States that feeds from the phloem tissue, drains nutrients, and inflicts physical damage to plants. Previously, it has been shown that SCA reproduction was low and high on sorghum SC265 and SC1345 plants, respectively, compared to RTx430, an elite sorghum male parental line (reference line). In this study, we focused on identifying the defense-related genes that confer resistance to SCA at early and late time points in sorghum plants with varied levels of SCA resistance.

Results We used RNA-sequencing approach to …


Ecology And Host Manipulation By An Egg-Larva Parasitoid Of Chrysodeixis Includens (Lepidoptera: Noctuidae), Daniel V.C. Neves, Renata R. Pereira, Julia N.D. Campos, Rodrigo S. Ramos, Paulo A.S. Junior, Daiane G. Carmo, Marcelo C. Picanço Jul 2023

Ecology And Host Manipulation By An Egg-Larva Parasitoid Of Chrysodeixis Includens (Lepidoptera: Noctuidae), Daniel V.C. Neves, Renata R. Pereira, Julia N.D. Campos, Rodrigo S. Ramos, Paulo A.S. Junior, Daiane G. Carmo, Marcelo C. Picanço

Department of Entomology: Faculty Publications

Parasitoids are among the main natural enemies of crop pests. Copidosoma truncatellum (Hymenoptera: Encyrtidae) is a parasitoid of Chrysodeixis includens (Walker) (Lepidoptera: Noctuidae: Plusiinae), which is an important pest of soybean, bean, cotton, sunflower, tomato and potato. Copidosoma are parasitoids of lepidopteran egg-larva, especially those of the subfamily Plusiinae. The embryonic development of the Copidosoma parasitoid begins in the lepidopteran egg, and this development extends to the beginning of the host larval stage of the parasitized lepidopteran. However, the rate of parasitism is a complex ecological relationship affected by climatic elements and age of the host. Thus, the present study …


Measuring Bee Effects On Seed Traits Of Hybrid Sunflower, Gary J. Brewer, Kentaro Miwa, Kathy Hanford Jul 2023

Measuring Bee Effects On Seed Traits Of Hybrid Sunflower, Gary J. Brewer, Kentaro Miwa, Kathy Hanford

Department of Entomology: Faculty Publications

In hybrid sunflower, bee pollination can improve productivity, but the contribution of bees to productivity may be over or underestimated. To estimate bee effects (seed trait gains from exposure to bees during anthesis), single capitula are commonly covered with a porous material to exclude bees. However, depending on the exclosure porosity, estimates of the magnitude of bee effects will vary. In two studies, porosity size and bee effect gains in two sunflower types were tested. In the exclosure study, Delnet exclosures severely reduced seed set and exclosures with larger porosities and had smaller and similar effects. However, since a few …


Effects Of Smartstax® And Smartstax® Pro Maize On Western Corn Rootworm (Diabrotica Virgifera Virgifera Leconte) Larval Feeding Injury And Adult Life History Parameters, Jordan D. Reinders, William J. Moar, Graham P. Head, Safeer Hassan, Lance J. Meinke Jul 2023

Effects Of Smartstax® And Smartstax® Pro Maize On Western Corn Rootworm (Diabrotica Virgifera Virgifera Leconte) Larval Feeding Injury And Adult Life History Parameters, Jordan D. Reinders, William J. Moar, Graham P. Head, Safeer Hassan, Lance J. Meinke

Department of Entomology: Faculty Publications

Field-evolved resistance of the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, to Bacillus thuringiensis Berliner (Bt) proteins Cry3Bb1 and Cry34/35Ab1 (now classified as Gpp34Ab1/Tpp35Ab1) expressed in the pyramid SmartStax® has been documented in areas of the United States (U.S.) Corn Belt. SmartStax® PRO is a recently registered rootworm-active pyramid containing the same Bt proteins expressed in SmartStax® plus DvSnf7 dsRNA. Little to no published data is available comparing efficacy of the technologies or potential effects of dietary exposure on adult WCR fitness. Therefore, experiments were conducted to compare effects of adult WCR dietary exposure to SmartStax …


Extended Sentinel Monitoring Of Helicoverpa Zea Resistance To Cry And Vip3aa Toxins In Bt Sweet Corn: Assessing Changes In Phenotypic And Allele Frequencies Of Resistance, Galen P. Dively, Tom P. Kuhar, Sally V. Taylor, Helene Doughty, Kristian Holmstrom, Daniel O. Gilrein, Brian A. Nault, Joseph Ingerson-Mahar, Anders Huseth, Dominic Reisig, Shelby Fleischer, David Owens, Kelley Tilmon, Francis Reay-Jones, Pat Porter, Jocelyn Smith, Julien Saguez, Jason Wells, Caitlin Congdon, Holly Byker, Bryan Jensen, Chris Difonzo, William D. Hutchison, Eric Burkness, Robert Wright, Michael Crossley, Heather Darby, Tom Bilbo, Nicholas Seiter, Christian Krupke, Craig Abel, Brad S. Coates, Bradley Mcmanus, Billy Fuller, Jeffrey Bradshaw, Julie A. Peterson, David Buntin, Silvana Paula-Moraes, Katelyn Kesheimer, Whitney Crow Jul 2023

Extended Sentinel Monitoring Of Helicoverpa Zea Resistance To Cry And Vip3aa Toxins In Bt Sweet Corn: Assessing Changes In Phenotypic And Allele Frequencies Of Resistance, Galen P. Dively, Tom P. Kuhar, Sally V. Taylor, Helene Doughty, Kristian Holmstrom, Daniel O. Gilrein, Brian A. Nault, Joseph Ingerson-Mahar, Anders Huseth, Dominic Reisig, Shelby Fleischer, David Owens, Kelley Tilmon, Francis Reay-Jones, Pat Porter, Jocelyn Smith, Julien Saguez, Jason Wells, Caitlin Congdon, Holly Byker, Bryan Jensen, Chris Difonzo, William D. Hutchison, Eric Burkness, Robert Wright, Michael Crossley, Heather Darby, Tom Bilbo, Nicholas Seiter, Christian Krupke, Craig Abel, Brad S. Coates, Bradley Mcmanus, Billy Fuller, Jeffrey Bradshaw, Julie A. Peterson, David Buntin, Silvana Paula-Moraes, Katelyn Kesheimer, Whitney Crow

Department of Entomology: Faculty Publications

Transgenic corn and cotton that produce Cry and Vip3Aa toxins derived from Bacillus thuringiensis (Bt) are widely planted in the United States to control lepidopteran pests. The sustainability of these Bt crops is threatened because the corn earworm/bollworm, Helicoverpa zea (Boddie), is evolving a resistance to these toxins. Using Bt sweet corn as a sentinel plant to monitor the evolution of resistance, collaborators established 146 trials in twenty-five states and five Canadian provinces during 2020–2022. The study evaluated overall changes in the phenotypic frequency of resistance (the ratio of larval densities in Bt ears relative to densities in non-Bt ears) …


Arthropod Diversity In Phytotelmata Of Calathea Capitata (Zingiberales; Marantaceae) Host Plants From Peru, Divya Dendi, Timo Förster, Caroline S. Chaboo Jun 2023

Arthropod Diversity In Phytotelmata Of Calathea Capitata (Zingiberales; Marantaceae) Host Plants From Peru, Divya Dendi, Timo Förster, Caroline S. Chaboo

Department of Entomology: Faculty Publications

Cryptic habitats are often overlooked in biodiversity surveys. Phytotelmata, or plant pools, are one type of cryptic habitat that supports diverse fauna in a miniature ecosystem. This study surveys the arthropod community of two types of phytotelmata, bracts and leaf rolls, on a single species, Calathea capitata (Ruiz and Pav.) Lindl. (Zingiberales: Marantaceae), from one Amazon site in Peru. Specimens were collected from eight bracts and eight leaf rolls. A total of 55 arthropods (36 adults, 19 juveniles) were found in both phytotelmata types. In the bract samples were found: spiders (Araneae: Corinnidae), beetles (Coleoptera: Carabidae, Erotylidae, Staphylindae), flies (Diptera: …


Movement Of Striacosta Albicosta (Smith) (Lepidoptera: Noctuidae) Larvae On Transgenic Bt And Non-Bt Maize, Débora G. Montezano, Thomas E. Hunt, Priscila M. Colombo Da Luz, Kelsey Karnik, Stephen D. Kachman, Ana Maria Velez, Julie A. Peterson Jun 2023

Movement Of Striacosta Albicosta (Smith) (Lepidoptera: Noctuidae) Larvae On Transgenic Bt And Non-Bt Maize, Débora G. Montezano, Thomas E. Hunt, Priscila M. Colombo Da Luz, Kelsey Karnik, Stephen D. Kachman, Ana Maria Velez, Julie A. Peterson

Department of Entomology: Faculty Publications

Exposure of lepidopteran pests to Bacillus thuringiensis (Bt) proteins has been shown to affect the behavior of larvae, including increased movement and avoidance of Bt-expressing plants or diet. Therefore, we hypothesized that the behavior of western bean cutworm, Striacosta albicosta (Smith) (Lepidoptera: Noctuidae), an important pest of maize, could be affected when exposed to Bt plants. To test this hypothesis, we conducted a series of artificial arena and on-plant experiments to determine S. albicosta neonate behavior when exposed to Bt and non-Bt plant tissue. Video tracking experiments presented neonate larvae with the choice of Bt or non- …


A Push–Pull Strategy To Suppress Stable Fly (Diptera: Muscidae) Attacks On Pasture Cattle Via A Coconut Oil Fatty Acid Repellent Formulation And Traps With M-Cresol Lures, Alexander T. Lehmann, Gary J. Brewer, David J. Boxler, Junwei J. Zhu, Kathy Hanford, David Taylor, James A. Kenar, Steven C. Cermak, Jerome A. Hogsette Mar 2023

A Push–Pull Strategy To Suppress Stable Fly (Diptera: Muscidae) Attacks On Pasture Cattle Via A Coconut Oil Fatty Acid Repellent Formulation And Traps With M-Cresol Lures, Alexander T. Lehmann, Gary J. Brewer, David J. Boxler, Junwei J. Zhu, Kathy Hanford, David Taylor, James A. Kenar, Steven C. Cermak, Jerome A. Hogsette

Department of Entomology: Faculty Publications

BACKGROUND: Stable flies [Stomoxys calcitrans (L.)] are economically important pests of cattle and other livestock. As an alternative to conventional insecticides, we tested a push–pull management strategy using a coconut oil fatty acid repellent formulation and an attractant-added stable fly trap.

RESULTS: In our field trials we found that weekly applications of a push–pull strategy can reduce stable fly populations on cattle as well as a standard insecticide (permethrin). We also found that the efficacy periods of the push–pull and permethrin treatments following on-animal application were equivalent. Traps with an attractant lure used as the pull component of the …


Association Of Excessive Precipitation And Agricultural Land Use With Honey Bee Colony Performance, Gabriela M. Quinlan, Rufus Isaacs, Clint R. V. Otto, Autumn H. Smart, Meghan O. Milbrath Mar 2023

Association Of Excessive Precipitation And Agricultural Land Use With Honey Bee Colony Performance, Gabriela M. Quinlan, Rufus Isaacs, Clint R. V. Otto, Autumn H. Smart, Meghan O. Milbrath

Department of Entomology: Faculty Publications

Context From landscape variables to weather, multiple environmental factors affect honey bees and other pollinators. Detailed honey bee colony assessments in a variety of landscape and weather conditions offer the opportunity to develop a mechanistic understanding of how landscape composition, configuration, and weather are associated with colony nutrition, demography, and productivity.

Objectives Our objective was to test if weather and landscape characteristics (e.g., agricultural versus forested land use) are associated with different honey bee colony outcomes (foraged nectar mass, foraged pollen mass, pupal population size, and adult population size change).

Methods We collected detailed colony measurements on over 450 honey …


A Draft Diabrotica Virgifera Virgifera Genome: Insights Into Control And Host Plant Adaption By A Major Maize Pest Insect, Brad S. Coates, Kimberly K. O. Walden, Dimpal Lata, Neetha Nanoth Vellichirammal, Robert F. Mitchell, Martin N. Andersson, Rachel Mckay, Marcé D. Lorenzen, Nathaniel Grubbs, Yu‑Hui Wang, Jinlong Han, Jing Li Xuan, Peter Willadsen, Huichun Wang, B. Wade French, Raman Bansal, Sammy Sedky, Dariane Souza, Dakota Bunn, Lance J. Meinke, Nicholas J. Miller, Blair D. Siegfried11,, Thomas W. Sappington, Hugh M. Robertson Jan 2023

A Draft Diabrotica Virgifera Virgifera Genome: Insights Into Control And Host Plant Adaption By A Major Maize Pest Insect, Brad S. Coates, Kimberly K. O. Walden, Dimpal Lata, Neetha Nanoth Vellichirammal, Robert F. Mitchell, Martin N. Andersson, Rachel Mckay, Marcé D. Lorenzen, Nathaniel Grubbs, Yu‑Hui Wang, Jinlong Han, Jing Li Xuan, Peter Willadsen, Huichun Wang, B. Wade French, Raman Bansal, Sammy Sedky, Dariane Souza, Dakota Bunn, Lance J. Meinke, Nicholas J. Miller, Blair D. Siegfried11,, Thomas W. Sappington, Hugh M. Robertson

Department of Entomology: Faculty Publications

Background Adaptations by arthropod pests to host plant defenses of crops determine their impacts on agricultural production. The larval host range of western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae), is restricted to maize and a few grasses. Resistance of D. v. virgifera to crop rotation practices and multiple insecticides contributes to its status as the most damaging pest of cultivated maize in North America and Europe. The extent to which adaptations by this pest contributes to host plant specialization remains unknown.

Results A 2.42 Gb draft D. v. virgifera genome, Dvir_v2.0, was assembled from short shotgun reads and scaffolded …


Characterization Of Western Corn Rootworm (Coleoptera: Chrysomelidae) Susceptibility To Foliar Insecticides In Northeast Nebraska, Timothy B. Dang, Ana M. Vélez, Arnubio Valencia-Jiménez, Jordan Reinders, Emily E. Stricklin, Matthew W. Carroll, Clinton D. Pilcher, Jeffrey T. Krumm, Graham P. Head, Lance Meinke Jan 2023

Characterization Of Western Corn Rootworm (Coleoptera: Chrysomelidae) Susceptibility To Foliar Insecticides In Northeast Nebraska, Timothy B. Dang, Ana M. Vélez, Arnubio Valencia-Jiménez, Jordan Reinders, Emily E. Stricklin, Matthew W. Carroll, Clinton D. Pilcher, Jeffrey T. Krumm, Graham P. Head, Lance Meinke

Department of Entomology: Faculty Publications

Foliar-applied insecticides are commonly used for adult Western Corn Rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), control in Nebraska but little efficacy data is available. Anecdotal reports of reduced efficacy in areas of northeast Nebraska led to the conduct of this study. Objectives were to (i) evaluate the efficacy of commercial applications of commonly used formulated insecticides (bifenthrin, lambda-cyhalothrin, chlorpyrifos, or tank mixes) for WCR control in 7 northeast Nebraska counties during 2019 and 2020 and (ii) conduct adult WCR concentration-response vial bioassays with bifenthrin, chlorpyrifos, and dimethoate active ingredients on a subset of field populations. Whole plant counts …


Insect And Pest Management For Sustaining Crop Production Under Changing Climatic Patterns Of Drylands, Ruchika Kashyap, Sajjan Grover, Heena Puri, Sandeep Kaur, Jagmohan Singh, Karansher S. Sandhu, Gagandeep Kaur, Hinal Kharva, Shivreet Kaur, Balwinder Kaur Jan 2023

Insect And Pest Management For Sustaining Crop Production Under Changing Climatic Patterns Of Drylands, Ruchika Kashyap, Sajjan Grover, Heena Puri, Sandeep Kaur, Jagmohan Singh, Karansher S. Sandhu, Gagandeep Kaur, Hinal Kharva, Shivreet Kaur, Balwinder Kaur

Department of Entomology: Faculty Publications

Climate change is alarming, particularly for agriculturists as it severely impacts the development, distribution, and survival of insects and pests, affecting crop production globally. Over time, climate change is drastically tumbling the crop productivity in all the cropping systems, whereas the dryland agriculture with existing low productivity is immensely hit. While all the existing species in drylands, including humans, are coping with extreme climate variations for millennia, future climate change predictions put dryland agriculture in a threat zone. Drylands support 38% of the world’s population; therefore, climate change coupled with population growth and global food security draws the attention of …


Dynamic Regulation Of Phenylpropanoid Pathway Metabolites In Modulating Sorghum Defense Against Fall Armyworm, Sajjan Grover, Sanket Shinde, Heena Puri, Nathan Palmer, Gautam Sarath, Scott E. Sattler, Joe Louis Nov 2022

Dynamic Regulation Of Phenylpropanoid Pathway Metabolites In Modulating Sorghum Defense Against Fall Armyworm, Sajjan Grover, Sanket Shinde, Heena Puri, Nathan Palmer, Gautam Sarath, Scott E. Sattler, Joe Louis

Department of Entomology: Faculty Publications

Plants undergo dynamic metabolic changes at the cellular level upon insect infestation to better defend themselves. Phenylpropanoids, a hub of secondary plant metabolites, encompass a wide range of compounds that can contribute to insect resistance. Here, the role of sorghum (Sorghum bicolor) phenylpropanoids in providing defense against the chewing herbivore, fall armyworm (FAW), Spodoptera frugiperda, was explored. We screened a panel of nested association mapping (NAM) founder lines against FAW and identified SC1345 and Ajabsido as most resistant and susceptible lines to FAW, respectively, compared to reference parent, RTx430. Gene expression and metabolomic studies suggested that FAW …


Uses And Benefits Of Algae As A Nutritional Supplement For Honey Bees, Benjamin J. Nichols, Vincent A. Ricigliano Nov 2022

Uses And Benefits Of Algae As A Nutritional Supplement For Honey Bees, Benjamin J. Nichols, Vincent A. Ricigliano

Department of Entomology: Faculty Publications

Honey bees are essential agricultural pollinators that are threatened by various interacting stressors, posing risks to beekeeping industries and human food security. Malnutrition is a major factor underlying managed bee colony losses that can be countered by feeding artificial diets, which aim to deliver essential macro- and micronutrients. Current bee nutritional supplements show room for improvement and require resources that compete with human food production. Algae and microalgae in particular have been gaining traction in the literature as alternative feed sources and nutritional supplements for livestock, including honey bees. Herein, we review the current literature and categorize the effects of …


Bee‑Safe Peptidomimetic Acaricides Achieved By Comparative Genomics, Vikas Jindal, Daqi Li, Leslie Catherine Rault, Soheila Fatehi, Rupinder Singh, Moritz Mating, Ye Zou, Ho‑Leung Ng, Krzysztof Kaczmarek, Janusz Zabrocki, Shunhua Gui, Guy Smagghe, Troy D. Anderson, Ronald J. Nachman, Yoonseong Park Oct 2022

Bee‑Safe Peptidomimetic Acaricides Achieved By Comparative Genomics, Vikas Jindal, Daqi Li, Leslie Catherine Rault, Soheila Fatehi, Rupinder Singh, Moritz Mating, Ye Zou, Ho‑Leung Ng, Krzysztof Kaczmarek, Janusz Zabrocki, Shunhua Gui, Guy Smagghe, Troy D. Anderson, Ronald J. Nachman, Yoonseong Park

Department of Entomology: Faculty Publications

The devastating Varroa mite (Varroa destructor Anderson and Trueman) is an obligatory ectoparasite of the honey bee, contributing to significant colony losses in North America and throughout the world. The limited number of conventional acaricides to reduce Varroa mites and prevent disease in honey bee colonies is challenged with wide-spread resistance and low target-site selectivity. Here, we propose a biorational approach using comparative genomics for the development of honey beesafe and selective acaricides targeting the Varroa mite-specific neuropeptidergic system regulated by proctolin, which is lacking in the honey bee. Proctolin is a highly conserved pentapeptide RYLPT (Arg-Tyr-Leu-Pro-Thr) known to …


Different Gut Microbiomes Of Developmental Stages Of Field-Collected Native And Invasive Western Bean Cutworm, Striacosta Albicosta, In Western Nebraska, Paul A. Ayayee, Austin Currie, Julie A. Peterson Sep 2022

Different Gut Microbiomes Of Developmental Stages Of Field-Collected Native And Invasive Western Bean Cutworm, Striacosta Albicosta, In Western Nebraska, Paul A. Ayayee, Austin Currie, Julie A. Peterson

Department of Entomology: Faculty Publications

While insects harbor gut microbial associates that perform various functions for the host, lepidopterans have not been considered as prime examples of having such relationships. The western bean cutworm, Striacosta albicosta (Lepidoptera: Noctuidae), is native to North America and has historically been a significant corn pest in its western distribution. It is currently expanding eastwards and is invasive in these new regions. Using 16S rRNA gene sequencing data, this study focused on characterizing the microbiota associated with field-collected eggs, larvae, adults, and host plant materials of S. albicosta in its native range. The diversity of microbiomes varied significantly among S. …


Pest Population Dynamics Are Related To A Continental Overwintering Gradient, Douglas Lawton, Anders S. Huseth, George G. Kennedy, Amy C. Morey, William D. Hutchison, Dominic D. Reisig, Seth J. Dorman, De Shae Dillard, Robert C. Venette, Russell L. Groves, John J. Adamczyk, Izailda Barbosa Dos Santos, Tracey Baute, Sebe Brown, Eric Burkness, Ashley Dean, Galen P. Dively, Hélène B. Doughty, Shelby J. Fleischer, Jessica Green, Jeremy K. Greene, Krista Hamilton, Erin Hodgson, Thomas Hunt, David Kerns, Billy Rogers Leonard, Sean Malone, Fred Musser, David Owens, John C. Palumbo, Silvana Paula-Moraes, Julie A. Peterson, Ricardo Ramirez, Silvia I. Rondon, Tracy L. Schilder, Abby Seaman, Lori Spears, Scott D. Stewart, Sally Taylor, Tyler Towles Sep 2022

Pest Population Dynamics Are Related To A Continental Overwintering Gradient, Douglas Lawton, Anders S. Huseth, George G. Kennedy, Amy C. Morey, William D. Hutchison, Dominic D. Reisig, Seth J. Dorman, De Shae Dillard, Robert C. Venette, Russell L. Groves, John J. Adamczyk, Izailda Barbosa Dos Santos, Tracey Baute, Sebe Brown, Eric Burkness, Ashley Dean, Galen P. Dively, Hélène B. Doughty, Shelby J. Fleischer, Jessica Green, Jeremy K. Greene, Krista Hamilton, Erin Hodgson, Thomas Hunt, David Kerns, Billy Rogers Leonard, Sean Malone, Fred Musser, David Owens, John C. Palumbo, Silvana Paula-Moraes, Julie A. Peterson, Ricardo Ramirez, Silvia I. Rondon, Tracy L. Schilder, Abby Seaman, Lori Spears, Scott D. Stewart, Sally Taylor, Tyler Towles

Department of Entomology: Faculty Publications

Overwintering success is an important determinant of arthropod populations that must be considered as climate change continues to influence the spatiotemporal population dynamics of agricultural pests. Using a long-term monitoring database and biologically relevant overwintering zones, we modeled the annual and seasonal population dynamics of a common pest, Helicoverpa zea (Boddie), based on three overwintering suitability zones throughout North America using four decades of soil temperatures: the southern range (able to persist through winter), transitional zone (uncertain overwintering survivorship), and northern limits (unable to survive winter). Our model indicates H. zea population dynamics are hierarchically structured with continental-level effects that …


Co-Transcriptomic Analysis Of The Maize–Western Corn Rootworm Interaction, Lise Pingault, Saumik Basu, Neetha N. Vellichirammal, William Paul Williams, Gautam Sarath, Joe Louis Sep 2022

Co-Transcriptomic Analysis Of The Maize–Western Corn Rootworm Interaction, Lise Pingault, Saumik Basu, Neetha N. Vellichirammal, William Paul Williams, Gautam Sarath, Joe Louis

Department of Entomology: Faculty Publications

The Western corn rootworm (WCR; Diabrotica virgifera virgifera) is an economically important belowground pest of maize. Belowground feeding by WCR is damaging because it weakens the roots system, diminishes nutrient uptake, and creates entry points for fungal and bacterial pathogens and increases lodging, all of which can significantly suppress maize yields. Previously, it was demonstrated that belowground herbivory can trigger plant defense responses in the roots and the shoots, thereby impacting intraplant communication. Although several aspects of maize-WCR interactions have been reported, co-transcriptomic remodeling in the plant and insect are yet to be explored. We used a maize genotype, …


Grassy–Herbaceous Land Moderates Regional Climate Effects On Honey Bee Colonies In The Northcentral Us, Gabriela M. Quinlan, Douglas Sponsler, Hannah R. Gaines-Day, Harper B G Mcminn-Sauder, Clint R V Otto, Autumn H. Smart, Théotime Colin, Claudio Gratton, Rufus Isaacs, Reed Johnson, Meghan O. Milbrath, Christina M. Grozinger Jun 2022

Grassy–Herbaceous Land Moderates Regional Climate Effects On Honey Bee Colonies In The Northcentral Us, Gabriela M. Quinlan, Douglas Sponsler, Hannah R. Gaines-Day, Harper B G Mcminn-Sauder, Clint R V Otto, Autumn H. Smart, Théotime Colin, Claudio Gratton, Rufus Isaacs, Reed Johnson, Meghan O. Milbrath, Christina M. Grozinger

Department of Entomology: Faculty Publications

The lack of seasonally sustained floral resources (i.e. pollen and nectar) is considered a primary global threat to pollinator health. However, the ability to predict the abundance of flowering resources for pollinators based upon climate, weather, and land cover is difficult due to insufficient monitoring over adequate spatial and temporal scales. Here we use spatiotemporally distributed honey bee hive scales that continuously measure hive weights as a standardized method to assess nectar intake. We analyze late summer colony weight gain as the response variable in a random forest regression model to determine the importance of climate, weather, and land cover …


Characterizing The Sublethal Effects Of Smartstax Pro Dietary Exposure On Life History Traits Of The Western Corn Rootworm, Diabrotica Virgifera Virgifera Leconte, Jordan D. Reinders, Emily E. Reinders, Emily Robinson, William J. Moar, Paula A. Price, Graham P. Head, Lance Meinke May 2022

Characterizing The Sublethal Effects Of Smartstax Pro Dietary Exposure On Life History Traits Of The Western Corn Rootworm, Diabrotica Virgifera Virgifera Leconte, Jordan D. Reinders, Emily E. Reinders, Emily Robinson, William J. Moar, Paula A. Price, Graham P. Head, Lance Meinke

Department of Entomology: Faculty Publications

The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, is an economically important pest of field corn (Zea mays L.) across the United States (U.S.) Corn Belt. Repeated use of transgenic hybrids expressing Bacillus thuringiensis (Bt) proteins has selected for field-evolved resistance to all current rootworm-active Bt proteins. The newest product available for WCR management is SmartStax® PRO, a rootworm-active pyramid containing Cry3Bb1, Cry34/35Ab1 [now reclassified as Gpp34Ab1/Tpp35Ab1] and a new mode of action, DvSnf7 dsRNA. Understanding the fitness of adult WCR after dietary exposure to SmartStax® PRO will identify potential impacts on WCR population dynamics and inform efforts …


Resource Amount And Discontinuity Influence Flight And Reproduction In Hippodamia Convergens (Coleoptera: Coccinellidae), Hannah E. Stowe, J. P. Michaud, Tania N. Kim May 2022

Resource Amount And Discontinuity Influence Flight And Reproduction In Hippodamia Convergens (Coleoptera: Coccinellidae), Hannah E. Stowe, J. P. Michaud, Tania N. Kim

Department of Entomology: Faculty Publications

Industrial-scale agriculture creates a mosaic of large monocultures in the landscape, where seasonal cropping cycles generate discontinuous resource availability for insect predators both spatially and temporally. In this environment, selection will favor predator movement and reproductive behaviors that optimize the location and effective utilization of resource (prey) pulses that are both patchy and ephemeral in nature. Using a model system to study predator movement and reproduction, we tested how discontinuous periods of food resource access that mimic fluctuating resource populations (aphids) would influence flight behavior and reproduction of a highly mobile predator, Hippodamia convergens (convergent lady beetle), and possibly modify …


Optimization Of Sample Unit Size For Sampling Stink Bugs (Hemiptera: Pentatomidae) In Soybean, Arthur V. Ribeiro, Rafael Carlesso Aita, Daniela T. Pezzini, Christina D. Difonzo, Thomas Hunt, Janrt J. Knodel, Christian H. Krupke, Lia Marchi-Werle, Andrew P. Michel, Nicholas J. Seiter, Robert J. Wright, William D. Hutchison, Robert L. Koch Apr 2022

Optimization Of Sample Unit Size For Sampling Stink Bugs (Hemiptera: Pentatomidae) In Soybean, Arthur V. Ribeiro, Rafael Carlesso Aita, Daniela T. Pezzini, Christina D. Difonzo, Thomas Hunt, Janrt J. Knodel, Christian H. Krupke, Lia Marchi-Werle, Andrew P. Michel, Nicholas J. Seiter, Robert J. Wright, William D. Hutchison, Robert L. Koch

Department of Entomology: Faculty Publications

Cost-effective and reliable sampling procedures are crucial for integrated pest management. Sweep net sampling is commonly used for stink bugs (Hemiptera: Pentatomidae) in soybean, with sample size being the number of sets of sweeps, and sample unit size the number of sweeps in each set. Sample unit size has received little attention, but can affect sampling parameters. Here, two sample unit sizes (10 vs. 25 sweeps) were compared for the sampling of stink bug taxa. On average, sampling for stink bugs took 3.6 more minutes with the 25-sweep than with the 10-sweep sample unit size. Generally, estimates of the mean …


Impact Of The Timing And Use Of An Insecticide On Arthropods In Cover-Crop-Corn Systems, Gabriela Inveninato Carmona, Emily Robinson, Julia Nogueira Duarte Campos, Anthony Justin Mcmechan Mar 2022

Impact Of The Timing And Use Of An Insecticide On Arthropods In Cover-Crop-Corn Systems, Gabriela Inveninato Carmona, Emily Robinson, Julia Nogueira Duarte Campos, Anthony Justin Mcmechan

Department of Entomology: Faculty Publications

Cover crops provide a habitat for pests and beneficial arthropods. Unexpected pest pressure in a cover-crop-to-corn system can occur and result in increased use of insecticides. Eight site-years of on-farm field studies were conducted in 2019, 2020, and 2021. The objective of the study was to evaluate the impact of insecticide timing relative to cover-crop termination on arthropod activity in a cover-crop-to-corn system. The treatments consisted of (i) glyphosate to terminate the cover crop, (ii) glyphosate and pyrethroid tank mix to terminate the cover crop, and (iii) glyphosate to terminate the cover crop and pyrethroid application 25 days after the …


Mobility Of Phosphine-Susceptible And -Resistant Rhyzopertha Dominica (Coleoptera: Bostrichidae) And Tribolium Castaneum (Coleoptera: Tenebrionidae) After Exposure To Controlled Release Materials With Existing And Novel Active Ingredients, Sabita Ranabhat, Kun Yan Zhu, Georgina Bingham, William R., Morrison Iii Feb 2022

Mobility Of Phosphine-Susceptible And -Resistant Rhyzopertha Dominica (Coleoptera: Bostrichidae) And Tribolium Castaneum (Coleoptera: Tenebrionidae) After Exposure To Controlled Release Materials With Existing And Novel Active Ingredients, Sabita Ranabhat, Kun Yan Zhu, Georgina Bingham, William R., Morrison Iii

Department of Entomology: Faculty Publications

There is interest in developing controlled release materials (CRMs) with novel modes of action to improve resistance management. Long-lasting insecticide-incorporated netting (LLIN) with deltamethrin has been effectively used against stored-product pests. Here, we evaluated the efficacy of different CRMs (LLIN or packaging) with each of four active ingredients (AI) (deltamethrin, permethrin, indoxacarb, and dinotefuran) and compared them to control CRMs in reducing movement and increasing mortality of phosphine-susceptible and -resistant Rhyzopertha dominica and Tribolium castaneum. Adults were exposed for 0.5, 2, or 60 min, and movement was assessed immediately or after 24, or 168 h using video-tracking and Ethovision …


Evaluation Of Hermetic Storage Bags For The Preservation Of Yellow Maize In Poultry Farms In Dormaa Ahenkro, Ghana, Bismark Opoku, Enoch Adjei Osekre, George Opit, Augustine Bosomtwe, Georgina Bingham Jan 2022

Evaluation Of Hermetic Storage Bags For The Preservation Of Yellow Maize In Poultry Farms In Dormaa Ahenkro, Ghana, Bismark Opoku, Enoch Adjei Osekre, George Opit, Augustine Bosomtwe, Georgina Bingham

Department of Entomology: Faculty Publications

Using low-quality maize, resulting from insect pests and fungal attack, for formulating feed reduces chicken performance. This study evaluated the effectiveness of hermetic storage bags to keep insect pest and mycotoxin levels in check in yellow maize. The study was conducted in storehouses at three poultry farms in Dormaa Ahenkro, Bono Region, Ghana. The experiment was set up in a Randomized Complete Block Design with ZeroFly®Hermetic (ZFH), Purdue Improved Crop Storage (PICS), and Polypropylene (PP) bags as treatments. In each treatment, twelve 50 kg samples of untreated maize were each put in 100 kg capacity bags. Two bags in each …


Reprogramming Of Sorghum Proteome In Response To Sugarcane Aphid Infestation, Sajjan Grover, Juan Betancurt Cardona, Prince Zogli, Sophie Alvarez, Michael J. Naldrett, Scott E. Sattler, Joe Louis Jan 2022

Reprogramming Of Sorghum Proteome In Response To Sugarcane Aphid Infestation, Sajjan Grover, Juan Betancurt Cardona, Prince Zogli, Sophie Alvarez, Michael J. Naldrett, Scott E. Sattler, Joe Louis

Department of Entomology: Faculty Publications

Sugarcane aphid (SCA; Melanaphis sacchari Zehntner) is a key piercing-sucking pest of sorghum (Sorghum bicolor) that cause significant yield losses. While feeding on host plants, complex signaling networks are invoked from recognition of insect attack to induction of plant defenses. Consequently, these signaling networks lead to the production of insecticidal compounds or limited access of nutrients to insects. Previously, several studies were published on the transcriptomics analysis of sorghum in response to SCA infestation, but no information is available on the physiological changes of sorghum at the proteome level. We used the SCA resistant sorghum genotype SC265 for …


Wheat Transcriptomic Responses To Extended Feeding By Wheat Curl Mites, Lise Pingault, Tran Kim Ngan Luong, Joe Louis, Gary Hein Jan 2022

Wheat Transcriptomic Responses To Extended Feeding By Wheat Curl Mites, Lise Pingault, Tran Kim Ngan Luong, Joe Louis, Gary Hein

Department of Entomology: Faculty Publications

The economic importance of wheat and its contribution to human and livestock diets has been already demonstrated. However, wheat production is impacted by pests that induce yield reductions. Among these pests, wheat curl mite (WCM, Aceria tosichella Keifer) impacts wheat all around the world. WCM are tiny pests that feed within the whorl of developing leaves, and their feeding causes leaf curling by preventing them from unfurling. The curling of the leaves provides a protective niche for the WCM. Additionally, WCM are also the vector of serious viruses in wheat. Little is known regarding the impact of the WCM on …