Entomology Commons^™

Reference Gene Selection For Transcriptional Profiling In Cryptocercus Punctulatus, An Evolutionary Link Between Isoptera And Blattodea, Zhen Li, Xiangrui Li, Qingwen Zhang, Ling Yuan, Xuguo Zhou

Plant and Soil Sciences Faculty Publications

The subsocial life style and wood-feeding capability of Cryptocercus gives us an evolutionary key to unlock some outstanding questions in biology. With the advent of the Genomics Era, there is an unprecedented opportunity to address the evolution of eusociality and the acquisition of lignocellulases at the genetic level. However, to quantify gene expression, an appropriate normalization strategy is warranted to control for the non-specific variations among samples across different experimental conditions. To search for the internal references, 10 housekeeping genes from a gut transcriptome of a wood-feeding cockroach, Cryptocercus punctulatus, were selected as the candidates for the RT-qPCR analysis. The …

Nebline, Nov./Dec. 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Nebraska Extension Celebrates 20 Years of School Enrichment Nutrition Kits!

Recipe of the Month

Fun Winter Gardening Projects

Garden Guide: Things to Do This Month

Be a Master Gardener!

Upcoming Green Industry Conferences

Helping Foster a Growth Mindset in Young Children

Pesticide Applicator Trainings

Landlord/Tenant Lease & Crop Budget Workshops

Extension News

4-H Announcements for enrolled 4-H'ers or 4-H volunteers

Heart of 4-H Volunteer Award: Jodi Gabel

Heart of 4-H Volunteer Award: Sarah Lanik-Frain and Mike Frain

2020 4-H Horse Awards

Extension Calendar

Outgoing 4-H Council Members

Youth Science Day for Homeschooled Youth

Ag Society Annual Election & Meeting

Super …

Selection Of Reference Genes For Qrt-Pcr Analysis In Medicinal Plant Glycyrrhiza Under Abiotic Stresses And Hormonal Treatments, Yuping Li, Xiaoju Liang, Xuguo Zhou, Zhigeng Wu, Ling Yuan, Ying Wang, Yongqing Li

Entomology Faculty Publications

Best known as licorice, Glycyrrhiza Linn., a genus of herbaceous perennial legume, has been used as a traditional herbal medicine in Asia and a flavoring agent for tobacco and food industry in Europe and America. Abiotic stresses and hormonal treatments can significantly impact the development and metabolism of secondary metabolites in Glycyrrhiza. To better understand the biosynthesis of the trace-amount bioactive compounds, we first screened for the suitable reference genes for quantitative real-time reverse transcription PCR (qRT-PCR) analysis in Glycyrrhiza. The expression profiles of 14 candidate reference genes, including Actin1 (ACT), Clathrin complex AP1 (CAC), Cyclophilin (CYP), Heat-shock …

Identification Of Planktothrix (Cyanobacteria) Blooms And Effects On The Aquatic Macroinvertebrate Community In The Non-Tidal Potomac River, Usa, Joshua Henesy, Jennifer L. Wolny, John E. Mullican, Detbra S. Rosales, Joseph S. Pitula, Joseph W. Love

Virginia Journal of Science

Using transverse cross-sectional transects, a survey of 31 km of the non-tidal Potomac River was conducted from White’s Ferry, Virginia to Brunswick, Maryland, USA, between June and September in 2013 through 2015 to assess a recurring benthic cyanobacteria bloom. Abundant benthic cyanobacteria blooms were detected during the 2014 and 2015 sampling seasons and the primary taxon was identified morphologically and molecularly as Planktothrix cf. isothrix. When present, P. cf. isothrix blooms were concentrated from river center to the Maryland shoreline. This pattern was correlated with significantly greater benthic chlorophyll-a and phycocyanin concentrations. In an apparent response to …

Evaluation Of Systemic Imidacloprid And Herbicide Treatments On Flatheaded Borer (Coleoptera: Buprestidae) Management In Field Nursery Production, Karla M. Addesso, Jason B. Oliver, Nadeer N. Youssef, Donna C. Fare

Agricultural and Environmental Sciences Faculty Research

The flatheaded appletree borer, Chrysobothris femorata (Olivier) (Coleoptera: Buprestidae), and related species are deciduous tree pests. Female beetles prefer to oviposit at tree bases, and larvae tunnel beneath the bark, which weakens or kills young or newly transplanted trees. In the first objective of this study, Discus N/G (2.94% imidacloprid + 0.7% cyfluthrin) applied at six lower-than-labeled rates (0.0, 0.98, 1.97, 3.94, 5.91, and 7.87 ml/cm of average trunk dia.) was evaluated for protection of field-grown maples. A second objective evaluated imidacloprid with and without herbicides to assess the impact of weed competition at the tree base on insecticide effectiveness. …

The Impact Of Plant Essential Oils And Fine Mesh Row Covers On Flea Beetle (Chrysomelidae) Management In Brassicaceous Greens Production, Robert Brockman, Ryan Kuesel, Kendall Archer, Kyla O’Hearn, Neil Wilson, Delia W. Scott, Mark A. Williams, Ricardo Bessin, David J. Gonthier

Entomology Faculty Publications

Brassicaceous leafy greens are an important crop for small growers but are difficult to produce due to damage by flea beetles. Flea beetles are problematic for growers as they chew many small holes through leaves rendering produce unmarketable. We tested the efficacy of several essential oils, the woven-mesh row cover ProtekNet, and the spunbonded row cover Agribon, compared to organic and conventional insecticides and no spray controls in the spring and fall of 2019. We found that the two row cover treatments (Agribon and ProtekNet) provided the best control of flea beetles and associated damage. Thyme oil was highly phytotoxic …

Nebline, Sept./Oct. 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Fall Invaders: What You Can Do

Extension Partners With LPS on Handwashing Videos

Plant A Row for the Hungry

Recipe of the Month

Pruning Everbearing Raspberries

Seasonal Needle Drop on Evergreens

Garden Guide: Things to Do This Month

Disasters Don't Wait. Make Your Plan Today!

Extension Offers Training for Emergency Preparedness and Coping

Upcoming Early Childhood Trainings

Nitrate in Drinking Water

Heart of 4-H Volunteer Award: Tracy Pracheil

Heart of 4-H Volunteer Award: Amy Vander Woude

4-H Announcements for enrolled 4-H'ers or 4-H volunteers

4-H Information Night

State 4-H Horse Expo Top Results

4-H at Super Fair a Blend of …

Ailanthus Altissima Aqueous Extract Deters Spodoptera Frugiperda Oviposition, Ryan L. Wagner, Jordan A. Card

The Great Lakes Entomologist

Ailanthus altissima (Mill.) Swingle (Simaroubaceae), the tree of heaven, is a highly invasive tree species containing phytochemicals with a range of biological activities. Exposure to novel chemistry from invasive plant species may result in changes to established Lepidopteran behaviors, including feeding or oviposition. However, the impact of A. altissima chemistry on insect behavior has not been extensively explored. Therefore, A. altissima extract was tested for oviposition deterrence effects against Spodoptera frugiperda (Smith) (Noctuidae). Oviposition substrates were treated with water-soluble extractions of Zea mays (L), A. altissima, or a combination of both. Moths were then placed in chambers containing different …

Permethrin Residual Activity Against Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae) Attacks Following Field Aging And Simulated Rainfall Weathering, Matthew S. Brown, Karla M. Addesso, Fulya Baysal-Gurel, Nadeer N. Youssef, Jason B. Oliver

Agricultural and Environmental Sciences Faculty Research

Adult ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) bore into ornamental nursery trees resulting in trunk vascular tissue damage, which can potentially kill trees. Ambrosia beetle exposure to surface-applied insecticides is minimal after internal trunk galleries are formed, so effective management requires insecticide treatments to be applied near the time of infestation or to have residual activity on the bark. Tree trunk sections (bolts) were used to determine the effect of field aging or irrigation (i.e., simulated rainfall weathering) on permethrin residual activity against ambrosia beetles. In all experiments, 30-cm-long bolts from Liriodendron tulipifera L. (Magnoliales: Magnoliaceae) were hollowed and filled with …

Nebline, August 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Be an Active Family with Outdoor Activities

EXTENSION OFFICE OPEN

Recipes of the month

What You Need to Know About Having a Well Drilled

Almost Time for Fall Lawn Seeding

Chiggers: Itching in all the Wrong Places

Collect – Share – Practice: Family Emergency Communication Plans

August Blooming Perennial Flowers

Garden Guide: Things to Do This Month

Creating Reading Routines During the Summer Months

HEART OF 4-H

4-H Youth and Volunteers Sew 1,789 Hats and Masks for Bryan Health

4-H’ers Problem Solve Real-Life Situations at County Life Challenge

Virtual State Public Speaking Contest Results

State Fair to Focus on Showcasing …

Ecology Of The Roseau Cane Scale (Nipponaclerda Biwakoensis, Hemiptera: Aclerdidae) In Coastal Louisiana, Leslie Alejandra Aviles Lopez

LSU Master's Theses

Common reed, Phragmites australis, is the dominant plant in the Mississippi River Delta (MRD), Louisiana. Phragmites australis stands reduce soil erosion from wave action, protect the oil infrastructure, and freshwater habitats. In the fall of 2016, widespread reed die-backs in the MRD were associated with outbreaks of an invasive scale Nipponaclerda biwakoensis (Hemiptera: Aclerdidae). Due to the recent detection of the scale, there was limited knowledge of its ecology in the adventive range, and its impacts on P. australis lineages. Therefore, the objectives of my thesis were to determine (1) the host specificity of the N. biwakoensis in important economic …

Nebline, June/July 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Top 5 Ag Topics From 5 Years

7 Tips for Successfully Baking as a Family

Recipe of the Month

Timing Your Fruit Harvesting Apples, Pears & Plums

Paper Wasps and Yellow Jackets

Severe Weather Preparedness and COVID-19

Time to Look for Bagworms

Garden Guide: What to Do This Month

5 Tips for Working Remotely from Home and Caring for Children During COVID-19

HEART OF 4-H VOLUNTEER AWARD: Grace Kim

HEART OF 4-H VOLUNTEER AWARD: Greater Lincoln Obedience Club

4-H/FFA Livestock Booster Club Scholarships Due July 6

Video Companion Animal (and Cat and Pet Rabbit) Showmanship Entries Due Aug. 10

Horse …

Role Of Plant Volatile Organic Compounds (Vocs) In Seed Priming And Plant-Herbivore Interactions., Abhinav Kumar Maurya

Electronic Theses and Dissertations

This dissertation explores and expands the existing knowledge on the role of plant volatiles in facilitating seed priming and providing direct defense against herbivore. Although the roles of plant volatiles in priming a plant’s defenses and providing direct defense against pests is well known, information regarding their effects on seed and the fitness of future plants and direct toxicity to herbivores is understudied. This dissertation does a thorough examination of these two understudied aspects of plant volatiles and provides novel insight into the role of plant volatiles in seed priming and direct defense (Chapter I). Seeds in the soil can …

Nebline, May 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Vegetable Gardening for Beginners

"Sewing for Hospitals" Community Service Project

Extension Postponements, Cancellations & Changes

Making a Meal With What's on Hand

Recipe of the Month

2020 Cash Rent Survey

Pesticide Applicators: Deferment of License Recertification

When Thunder Roars or Lightening Flashes, Go Indoors!

Sleep Impacts Mental and Physical Health

2020 Perennial Plant of the Year

Garden Guide: Things to Do This Month

Composting How-To Videos

Spending Time in Nature is Beneficial to Children and Adults

4-H Announcements for enrolled 4-H'ers or 4-H volunteers

Heart of 4-H Volunteer Award: Mary Burroughs

Clover College Workshop Descriptions

Extension Calendar

Support Local 4-H …

Extreme Fire As A Management Tool To Combat Regime Shifts In The Range Of The Endangered American Burying Beetle, Alison K. Ludwig, Daniel R. Uden, Dirac Twidwell

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

This study is focused on the population of federally-endangered American burying beetles in south-central Nebraska. It is focused on changes in land cover over time and at several levels of spatial scale, and how management efforts are impacting both the beetle and a changing landscape. Our findings are applicable to a large portion of the Great Plains, which is undergoing the same shift from grassland to woodland, and to areas where the beetle is still found.

Larval Survival And Movement Of The Fall Armyworm And Corn Earworm (Lepidoptera: Noctuidae) In Seed Blends Of Non-Bt And Pyramided Bt Corn: Implications For Resistance Management, Marcelo Dimase

LSU Master's Theses

A seed blend refuge has been used in the U.S. Corn Belt for Bt corn insect resistance management (IRM). The fall armyworm, Spodoptera frugiperda (F.), and corn earworm, Helicoverpa zea (Boddie) are major target pests of Bt corn in the Americas. One major concern for the use of a seed blend refuge is that larval movement of insects among non-Bt and Bt plants could affect its efficacy for resistance management. To address this concern, field and greenhouse trials were conducted to determine 1) if seed blend refugia could provide comparable levels of susceptible S. frugiperda (aabb) as structured refugia and …

Nebline, April 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Preparing Water for Use in an Emergency

Extension Postponements, Cancellations & Changes

Census Reminder

Create a Culture of Wellness with Nebraska Extension

RECIPE OF THE MONTH

Warm Spring and Weed Growth

Creating Capable Children

Empathy Over Sympathy

Those Pesky Fruit Flies

Preventing Conflicts With Tree Squirrels

Six Annual Flowers for Sunny Locations

Hiring a Lawn & Landscape Professional

Garden Guide: Things to Do This Month

HEART OF 4-H

At 4-H Lock-In, Teen Council 4-H’ers “Inspire Kids to Do”

New in 4-H at Super Fair

4-H/FFA Livestock Identification Help Nights, June 2 & 9

4-H Senior Ellie Babcock’s Third Year Showcasing …

Ecological And Economic Benefits And Risks Of Using Botanical Insecticides In Tanzanian Farms, Lilia Garcia

Independent Study Project (ISP) Collection

Insect damage is a major concern for smallholder farmers in developing countries like Tanzania. Synthetic insecticides can tame infestations, however they can be expensive, inaccessible, and their misuse can threaten farmer health and ecological conditions. Botanical insecticides are cheap alternatives to treat infestations while preserving beneficial insects such as pollinators, predators, and parasitoids. This study assesses how both synthetic and botanical insecticides affect beneficial insects, crop yield and profit/costs. This study finds botanical insecticides slightly less harmful towards non-target insects. Botanical insecticides seldomly improve crop yields but usually result in a higher profit/lower cost. Due to high variation in ecological …

Using Degree Days To Time Treatments For Insect Pests, Marion Murray

All Current Publications

Insecticides that are applied for a perennial insect pest based on a calendar date often result in poor insect control and a waste of resources. Insect activity varies from year to year depending on weather. For example, in Logan, Utah, eggs of the apple pest codling moth began hatching on May 15 in 2005, May 5 in 2006, and April 30 in 2007.

Nebline, March 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Nutrition Education Program Fosters Healthy Families and Communities

Everyone Counts. 2020 Census

Recipes of the Month

Private Applicator Trainings, March 9 & 10

Southeast Nebraska Soil Health Conference, March 3

Dicamba Training, March 10

Recent Workshop Recordings Online

Pruning to Create Strength and Good Structure in Young Trees

Bug Word Search

Avoid Unwanted Guests at Your Bird Feeders

2020 All-America Selections Vegetable Winners

Garden Guide: Things to Do This Month

Look Who's Hatching Program for Childcare Centers "Incubates" Imagination

Heart of 4-H Volunteer Award: Clayton Haman

4-H Announcements for 4-H'ers and Volunteers

4-H Achievement Celebration

Extension Calendar

4-H Horse Hippology …

A History Of Zinnias: Flower For The Ages, Eric Grissell

Purdue University Press Book Previews

A History of Zinnias brings forward the fascinating adventure of zinnias and the spirit of civilization. With colorful illustrations, this book is a cultural and horticultural history documenting the development of garden zinnias—one of the top ten garden annuals grown in the United States today.

The deep and exciting history of garden zinnias pieces together a tale involving Aztecs, Spanish conquistadors, people of faith, people of medicine, explorers, scientists, writers, botanists, painters, and gardeners. The trail leads from the halls of Moctezuma to a cliff-diving prime minister; from Handel, Mozart, and Rossini to Gilbert and Sullivan; from a little-known confession …

Impacts Of Invasive Rats On Hawaiian Cave Resources, Francis G. Howarth, Fred D. Stone

International Journal of Speleology

Although there are no published studies and limited data documenting damage by rodents in Hawaiian caves, our incidental observations during more than 40 years of surveying caves indicate that introduced rodents, especially the roof rat, Rattus rattus, pose significant threats to vulnerable cave resources. Caves, with their nearly constant and predictable physical environment often house important natural and cultural features including biological, paleontological, geological, climatic, mineralogical, cultural, and archaeological resources. All four invasive rodents in Hawai‘i commonly nest in cave entrances and rock shelters, but only the roof rat (Rattus rattus) habitually enters caves and utilizes areas …

Nebline, February 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

High-Quality Early Child Care Is Important to Children and Communities

Taking Part is Your Civic Duty

Sweet Ideas Without Added Sugar

Berry Fun

Recipes of the Month

Pesticide Applicator Trainings

Growing Nut Trees Seminar, Feb. 20

Landlord/Tenant Cash Rent Workshop, Feb. 3

ProHort Lawn & Landscape Update

Southeast Nebraska Soil Health Conference, March 3

Backyard Bird Feeding Tips

Positive Change on Environmental Issues

What Do the Three Arrows of Recycling Mean?

2020 Flower All-America Selections

Garden Guide: Things to Do This Month

Lincoln Littles Early Childhood Education Initiative

Lincoln Early Childhood Conference

HEART OF 4-H

Meet 2019–2020 4-H Teen Council …

Letter From The Dean, Deacue Fields

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Integrated Crop–Livestock Systems In Paddy Fields: New Strategies For Flooded Rice Nutrition, Luiz Gustavo De O. Denardin, Amanda P. Martins, Felipe De C. Carmona, Murilo G. Veloso, Gabriela I. Carmona, Paulo César De F. Carvalho, Ibanor Anghinoni

Department of Entomology: Faculty Publications

Integrated crop–livestock systems (ICLSs) appear as a good alternative to increase nutrient use efficiency (NUE) in rice (Oryza sativa L.) through the improvement in nutrient cycling and soil chemical attributes in paddy fields. The objective of this study was to evaluate the impact of an ICLS on soil chemical attributes and on the fertilization requirement of N, P, and K by flooded rice in the Brazilian subtropical region. Nutritional status, yield, and NUE of flooded rice were evaluated by fertilization trials through rice response to different fertilization rates of N, P, and K. Soil chemical attributes were evaluated at …

Geographic Distribution Of Bacillus Thuringiensis Cry1f Toxin Resistance In Western Bean Cutworm (Lepidoptera: Noctuidae) Populations In The United States, Brad S. Coates, Craig A. Abel, Katharine A. Swoboda-Bhattarai, Debra E. Palmquist, Débora Goulart Montezano, Sarah N. Zukoff, Yangzhou Wang, Jeffery D. Bradshaw, Christina D. Difonzo, Elson Shields, Kelley J. Tilmon, Thomas Hunt, Julie A. Peterson

West Central Research and Extension Center, North Platte

The western bean cutworm (WBC), Striacosta albicosta (Lepidoptera: Noctuidae), can be a severe pest of transgenic corn in the western Plains and Great Lakes regions of North America, including on hybrids expressing the Bacillus thuringiensis (Bt) Cry1F toxin. The level and geographic distribution of Cry1F resistance are not completely known. Neonate S. albicosta from 10 locations between Nebraska and New York state were subjected to dose–response trypsin-activated native Cry1F toxin overlay bioassays. In 2017, the mean estimated lethal concentration causing 50% larval mortality (LC50) ranged from 15.1 to 18.4 μg Cry1F cm−2, and were not significantly different among locations. In …

Improving The Biological Control Of Persicaria Perfoliata (Polygonaceae) Using Rhinoncomimus Latipes Korotyaev (Coleoptera: Curculionidae), Jaewon Kim

Graduate Theses, Dissertations, and Problem Reports

Persicaria perfoliata (L.) H. Gross (Polygonaceae; Mile-a-minute weed) is a rapid-growing invasive vine introduced from eastern Asia to northeastern United States in the 1930s. This vine has been invaded in disturbed areas and reforestation sites in 15 states in the U.S. and forms dense, monocultural patches that may inhibit natural forest regeneration. To control this weed, a host-specific biocontrol agent, Rhinoncomimus latipes Korotyaev (Coleoptera: Curculionidae) has been released in the P. perfoliata invaded states in the U.S. during the past 15 years. Currently, R. latipes is released by hand to the invaded area where the presence of the weed is …

Letter From The Faculty And Managing Editors, Beth Kegley, Gail Halleck

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 21 2020, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Estimation Of Additive And Dominance Effects Of A Mutant Glutathione S-Transferase Gene On Anthocyanin Content In Muscadine Grape (Vitis Rotundifolia), Autumn Brown, Margaret Worthington, Aruna Varanasi, Lacy Nelson, Renee T. Threlfall, Luke R. Howard

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The skin color of muscadine grapes (Vitis rotundifolia) is typically classified as black or bronze. A glutathione S-transferase, VrunGST4, has been identified as a candidate gene for berry skin color in muscadine grapes. A molecular marker was developed within VrunGST4 to distinguish between muscadine genotypes (cultivars and selections) with bronze (T:T), heterozygote black (C:T), and homozygote black (C:C) berries. The objectives of this study were to determine whether there was a correlation between berry skin color and total anthocyanin content and to calculate additive and dominance effects of VrunGST4 in determining total anthocyanins in the berries of two biparental F1 …