Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Entomology
Molecular Phylogeny And Typing Of Blackflies (Diptera: Simuliidae) That Serve As Vectors Of Human Or Bovine Onchocerciasis, Jianming Tang, Kenneth Pruess, Eddie W. Cupp, Thomas R. Unnasch
Molecular Phylogeny And Typing Of Blackflies (Diptera: Simuliidae) That Serve As Vectors Of Human Or Bovine Onchocerciasis, Jianming Tang, Kenneth Pruess, Eddie W. Cupp, Thomas R. Unnasch
Department of Entomology: Faculty Publications
A subregion of the mitochondrial large subunit (16s) rRNA gene was amplified by polymerase chain reaction (PCR) from nine species of blackflies (Diptera: Simuliidae) which serve as natural or experimental vectors of human or bovine Onchocerca parasites. PCR products from each species of blackfly were tested by directed heteroduplex analysis (DHDA), and their genotypes established according to diagnostic banding patterns of the heteroduplex products. Three alleles of mitochondrial 16s rRNA were found to exist in members of the Simulium (Edwardsellum) damnosum sensu lato complex from West Africa, and two alleles were found in the Neotropical Simulium (Psilopelmia …