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Full-Text Articles in Entomology
Identification Of Muscidifurax Spp. By Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, David Taylor, Allen Szalanski
Identification Of Muscidifurax Spp. By Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, David Taylor, Allen Szalanski
David B. Taylor
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the nuclear ribosomal ITS1 region was used to differentiate Muscidifurax (Hymenoptera: Pteromalidae) species which are parasitoids of filth fly pupae. Three restriction enzymes, Dpn 11, Mse I, and Taq I, produced restriction patterns which were diagnostic for the four species analyzed, M. raptor, M. raptorellus, M. uniraptor, and M. zaraptor. Seven other restriction enzymes were able to differentiate one or more of the species and can be used alone, or in combination with other enzymes, to verify identifications. No intraspecific variation was observed among the populations examined. The utility of the …
Genetic Similarity Among Pheromone And Voltinism Races Of Ostrinia Nubilalis (Hubner) (Lepidoptera: Crambidae), P. C. R. G. Marcon, David B. Taylor, C. E. Mason, R. L. Hellmich, Blair Siegfried
Genetic Similarity Among Pheromone And Voltinism Races Of Ostrinia Nubilalis (Hubner) (Lepidoptera: Crambidae), P. C. R. G. Marcon, David B. Taylor, C. E. Mason, R. L. Hellmich, Blair Siegfried
David B. Taylor
The genetic variability of seven European corn borer populations, Ostrinia nubilalis, from North America and Europe was assessed by polymerase chain reaction- restriction fragment length polymorphism (PCRRFLP) analysis and DNA sequencing. The nuclear ribosomal internal transcribed spacer 1 (ITS-1) region (≈ 500 base pair [bp]) and four mitochondrial (mtDNA) regions (1550 bp total) were examined. The smartweed borer, Ostrinia obumbratalis, and south-Western corn borer, Diatraea grandiosella, were used for comparisons. Of 106 restriction sites identified (80 in mtDNA and 26 in ITS-1), none differentiated geographical populations, pheromone races, or voltine ecotypes of the European corn borer. The lack of variation …
Population Genetics And Gene Variation In Secondary Screwworm (Diptera: Calliphoridae), David Taylor, Richard Peterson Ii
Population Genetics And Gene Variation In Secondary Screwworm (Diptera: Calliphoridae), David Taylor, Richard Peterson Ii
David B. Taylor
Allozyme variation in 4 populations of secondary screwworm, Cochliomyia macellaria (F.), representing North, Central, and South America was examined . Variability was observed in 12 of 13 enzyme loci, and the frequency of the most common allele was <0.95 for 6 loci . Observed and expected heterozygosities were 0.149 and 0.154, respectively. Nei's genetic distances were ≤ 0.001. Goodness-of-fit statistics for Hardy-Weinberg equilibrium and Wright's FIS statistics indicated random mating within populations . Wright's FST statistics and chi-square contingency analyses indicated homogeneous gene frequencies among the populations. These data suggest that C. macellaria is a panmictic species with high levels …
Sugar Feeding In Adult Stable Flies, David Taylor, Dennis R. Berkebile
Sugar Feeding In Adult Stable Flies, David Taylor, Dennis R. Berkebile
David B. Taylor
Adult stable flies (Stomoxys calcitrans L.) are known to feed readily on sugars in the laboratory. However, little is known concerning the extent of stable fly sugar feeding in wild populations. We examined the frequency of sugar feeding in stable flies collected on Alsynite sticky traps in rural and urban environments. In addition, stable flies were visually examined to determine whether blood was present in the gut. In laboratory studies, sugars were detectable with the anthrone technique in stable flies for ~3 d after being imbibed, and blood could be visually detected in the gut for 24-48 h after feeding. …