Ecology and Evolutionary Biology Commons^™

Comparison Of Phenolic Compounds And Antioxidant Capacities Of Traditional Sorghum Beers With Other Alcoholic Beverages, Fatouma Abdoul-Latif, Romaric G. Bayili, Louis C. Obame, Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

Thirty samples of sorghum beers “dolo” were selected from traditionally fermented household manufacturers from Burkina Faso. Dolo samples were screened for their total phenolic content, proanthocyanidins and putative antioxidant capacities, and were compared with industrial beers and wines. Total phenols were measured using the Folin-Ciocalteu method. Proanthocyanidins content were determined by the method of HCl-butanol hydrolysis. Antioxidant activities were evaluated both with 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and by the trolox equivalent antioxidant capacity (TEAC) using 2,2’-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid radical) (ABTS•+). The average contents of total phenols and proanthocyanidins were 506 μg GAE/ml of dolo and 45 μg APE/ml of dolo, respectively. An …

Of Gardens, Microorganisms, And Long Island Sound, Judy Preston

Wrack Lines

How healthy soil can make an environmental difference.

"I happened to turn over a large rounded stone in my garden to find a mesmerizing world of organisms that had, just moments earlier, been going about their business before being exposed to the sunlit world, and me."

The Determinants Of Helminth Infection In Baboons, Rita Monfort

Publications and Research

THE DETERMINANTS OF HELMINTH INFECTION IN BABOONS

Summary Paragraph:

Intestinal parasitic helminths are common in wild primate populations [1, 2] and can impose a significant burden on their host’s fitness. Numerous factors can affect the prevalence and diversity of intestinal parasites in natural populations including environmental factors [3, 4], the host’s behavior and genetics [5, 6]. How these different factors interact in natural populations remains unclear. This is in all probability due to the fact that previous studies have seldom looked at the prevalence and diversity of parasitic helminths in the same species but in different habitats over periods longer …

Toxicity Assessment And Analgesic Activity Investigation Of Aqueous Acetone Extracts Of Sida Acuta Burn F. And Sida Cordifolia L. (Malvaceae), Medicinal Plants Of Burkina Faso, Kiessoum Konate, Adama Hilou, Raïssa Rr Aworet-Samseny, Alain Souza, Nicolas Barro, Mamoudou H. Dicko Prof., Jacques Datté, Bertrand M’Batchi

Pr. Mamoudou H. DICKO, PhD

Background Sida acuta Burn f. and Sida cordifolia L. (Malvaceae) are traditionally used in Burkina Faso to treat several ailments, mainly pains, including abdominal infections and associated diseases. Despite the extensive use of these plants in traditional health care, literature provides little information regarding their toxicity and the pharmacology. This work was therefore designed to investigate the toxicological effects of aqueous acetone extracts of Sida acuta Burn f. and Sida cordifolia L. Furthermore, their analgesic capacity was assessed, in order to assess the efficiency of the traditional use of these two medicinal plants from Burkina Faso. Method For acute toxicity …

Two Boundaries Separate Borrelia Burgdorferi Populations In North America, Gabriele Margos, Jean I. Tsao, Santiago Castillo-Ramirez, Yvette A. Girard, Anne G. Hoen

Dartmouth Scholarship

Understanding the spread of infectious diseases is crucial for implementing effective control measures. For this, it is important to obtain information on the contemporary population structure of a disease agent and to infer the evolutionary processes that may have shaped it. Here, we investigate on a continental scale the population structure of Borrelia burgdorferi, the causative agent of Lyme borreliosis (LB), a tick-borne disease, in North America. We test the hypothesis that the observed d population structure is congruent with recent population expansions and that these were preceded by bottlenecks mostly likely caused by the near extirpation in the 1900s …

Antibacterial Activity Against Beta- Lactamase Producing Methicillin And Ampicillin-Resistants Staphylococcus Aureus: Fractional Inhibitory Concentration Index (Fici) Determination, Kiessoun K Onate, Jacques François Mavoungou, Alexis Nicaise Lepengué, Raïssa Rr Aworet-Samseny, Adama Hilou, Mamoudou H. Dicko Prof., Bertrand M’Batchi

Pr. Mamoudou H. DICKO, PhD

The present study reports the antibacterial capacity of alkaloid compounds in combination with Methicillin and Ampicillin-resistants bacteria isolated from clinical samples. The resistance of different bacteria strains to the current antibacterial agents, their toxicity and the cost of the treatment have led to the development of natural products against the bacteria resistant infections when applied in combination with conventional antimicrobial drugs. The antibacterial assays in this study were performed by using inhibition zone diameters, MIC, MBC methods, the time-kill assay and the Fractional Inhibitory Concentration Index (FICI) determination. On the whole, fifteen Gram-positive bacterial strains (MRSA/ARSA) were used. Negative control …

Diversity And Activity Of Roseobacters And Roseophage, Charles Ryan Budinoff

Doctoral Dissertations

Bacteria of the Roseobacter lineage are dominant bacterioplankton in coastal systems and contribute significantly to secondary production in oceanic environments. Generalities of Roseobacter ecology, diversity, and distributions are known, but the intraspecific differences between species and their dynamics over short temporal periods is not well understood. Bacteriophage that infect Roseobacters (‘roseophage’) have the potential to shunt secondary production into the dissolved carbon pool and through the process of infection alter Roseobacter physiology. Despite their significance, little effort was made prior to the onset of this study to characterize roseophage. Using culture dependent and independent approaches, I describe the diversity and …

The Effects Of Nutrient Limitation And Cyanophage On Heterotrophic Microbial Diversity, Claire Elyse Campbell

Masters Theses

Marine viruses are critically important in the regulation of biogeochemical cycles and host microbial communities. In this study, we tested whether the indirect effects of virus predation on a phototroph (i.e., Synechococcus) affected the composition of co-occurring heterotrophic bacteria under nitrogen and phosphorus limitation in long-term chemostat experiments. Using 454 Titanium barcoded pyrosequencing of the 16S rRNA gene, microbial diversity and technical (i.e., sequencing) reproducibility were assessed for nine individual chemostats across five different time points. A total of 325,142 reads were obtained; 194,778 high-quality, non-cyanobacterial sequences were assigned to 110 OTUs. Our results show high reproducibility …

Effects Of The Parasitic Dinoflagellate Hematodinium Sp. On Blue Crab (Callinectes Sapidus) Activity Predation And Habitat Selection, John M. Tiggelaar Ii

Biological Sciences Theses & Dissertations

The blue crab Callinectes sapidus occurs along the Atlantic and Gulf coasts of the United States, and in high salinity regions, they are subject to lethal infection by the parasitic dinoflagellate Hematodinium. In Virginia's seaside estuaries, the prevalence of Hematodinium infection of C. sapidus can sometimes exceed 50%, threatening the commercial fishery for this species. Indeed, other commercially important crustacean fisheries have approached collapse due to Hematodinium infections. Most studies of this host-parasite interaction have focused on epidemiology, host-pathogen dynamics, and pathogen transmission, and little is known about the impact of the parasite on host behavior and population dynamics. …

Links From Mantle To Microbe At The Lau Integrated Study Site: Insights From A Back-Arc Spreading Center, Margaret K. Tivey, Erin Becker, Roxanne Beinart, Charles R. Fisher, Peter Girguis, Charles H. Langmuir, Peter J. Michael, Anna-Louise Reysenbach

Biology Faculty Publications and Presentations

The Lau Integrated Study Site (ISS) has provided unique opportunities for study of ridge processes because of its back-arc setting in the southwestern Pacific. Its location allows study of a biogeographical province distinct from those of eastern Pacific and mid-Atlantic ridges, and crustal compositions along the ridge lie outside the range of mid-ocean ridge crustal compositions. The Lau ISS is located above a subduction zone, at an oblique angle. The underlying mantle receives water and other elements derived from the downgoing lithospheric slab, with an increase in slab influence from north to south. Water lowers the mantle melting temperature and …

Bacteriocin Formation By Dominant Aerobic Sporeformers Isolated From Traditional Maari, Donatien Kabore, Line Thorsen, Dennis Sandris Nielsen, Torben Sune Berner, Hagrétou Sawadogo-Lingani, Bréhima Diawara, Mamoudou H. Dicko Prof., Mogens Jackobsen

Pr. Mamoudou H. DICKO, PhD

The antimicrobial activity of 8 Bacillus spp. and 2 Lysinibacillus spp. representing the predominant aerobic sporeformers during traditional maari fermentations, a traditional fermented baobab seeds product fromBurkina Faso,was investigated. The antimicrobial activitywas assessed against a total of 31 indicator organisms representing various Gram-negative and positive pathogens. The screening showed that 3 Bacillus subtilis strains (B3, B122 and B222) in particular had antimicrobial activity against some Gram-positive organisms and were selected for further studies. Itwas found that the antimicrobial substances producedwere heat stable, in-sensitive to catalase, sensitive to protease and trypsin but resistant to the proteolytic action of papain and proteinase …

Acid Resistance, Bile Tolerance And Antimicrobial Properties Of Dominant Lactic Acid Bacteria Isolated From Traditional “Maari” Baobab Seeds Fermented Condiment, Donatien Kabore, Hagrétou Sawadogo-Lingani, Mamoudou H. Dicko Prof., Bréhima Diawara, Mogens Jacobsen

Pr. Mamoudou H. DICKO, PhD

Maari is a fermented food condiment obtained by spontaneous fermentation of seeds from the baobab tree (Adansonia digitata). Nine dominant lactic acid bacteria (LAB) strains, isolated from traditional maari fermentation were examined for their resistance to pH 2.5, their tolerance to 0.3% bile and their antimicrobial activities against pathogenic bacteria. The agar spot test was used to screen the dominant LAB for antagonistic activity against a total of 21 indicator organisms including Bacillus cereus strains, Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, Escherichia coli and Micrococcus luteus. It was observed that all LAB strains survived in 0.3% bile and exhibited antimicrobial …

Bacterial Community Structure Of Contrasting Soils Underlying Bornean Rain Forests: Inferences From Microarray And Next-Generation Sequencing Methods, Sabrina E. Russo, Ryan Legge, Karrie A. Weber, Eoin L. Brodie, Katherine C. Goldfarb, Andrew K. Benson, Sylvester Tan

School of Biological Sciences: Faculty Publications

Soil microbial diversity is vast, and we lack even basic understanding of how this diversity is distributed ecologically. Using pyrosequencing and microarray methods, we quantified the structure of bacterial communities in two contrasting soils underlying Bornean rain forest (clay and sandy loam) that differ markedly in soil properties, aboveground tree flora, and leaf litter decomposition rates. We found significant soil-related taxonomic and phylogenetic differences between communities that, due to their proximity, are independent of climate. Bacterial communities showed distinct compositional and taxon-abundance distributions that were significantly correlated with the structure of the overlying tree community. Richness of bacteria was greater …

Control And Passive Treatment Of Runoff From Horse Muck Storage Structures Using Rain Gardens, Hillary K. Otte

Theses and Dissertations--Forestry and Natural Resources

Runoff from livestock operations may contain a variety of pathogens and high levels of nutrients and other harmful contaminants, and is of particular concern in central Kentucky as watersheds are threatened by waste generated from a high concentration of equine activity. Rain gardens are a type of stormwater management tool used to capture and passively treat runoff. This project aimed to incorporate rain gardens into the horse muck storage structures at a thoroughbred facility in the Canr Run watershed in Lexington, Kentucky. Water quality data from soil water within two rain garden muck pads and two control pads, and grab …

Science Needs More Moneyball, Frederick Cohan

Frederick M. Cohan

No abstract provided.

Demarcation Of Bacterial Ecotypes From Dna Sequence Data: A Comparative Analysis Of Four Algorithms, Juan Francisco, Frederick Cohan, Danny Krizanc

Frederick M. Cohan

No abstract provided.

Prokaryotic Sex: Eukaryote-Like Qualities Of Recombination In An Archaean Lineage, Frederick Cohan, Stephanie Aracena

Frederick M. Cohan

No abstract provided.

Toward The History Of Study Of Symbiogenesis: On The English Translation Of B. M. Kozo-Polyansky’S A New Principle Of Biology (1924), Victor Fet

Biological Sciences Faculty Research

We reproduce the text by Victor Fet, which was read on 6 October 2011 at the Moscow Society of Naturalists during the presentation of new book translation (B.M. Kozo- Polyansky. Symbiogenesis: A New Principle of Evolution / transl. by Victor Fet; ed. by Victor Fet & Lynn Margulis. Cambridge, Mass.: Harvard University Press, 2010. 138 p.) This half- forgotten book by Boris M. Kozo-Polyansky was known only by name to Western biologists. Victor Fet gives a brief history of this new translation, enthusiastically initiated and supported by Lynn Margulis (1938–2011), a famous naturalist who was always eager to gave credit …

Effects Of Sugar-Amendment On Bacterial & Fungal Abundance In Native Vs. Nonnative-Dominated Soils Of A Puget Lowland Prairie, Jessica Wong

Summer Research

Scotch broom is a nonnative plant that has invaded the plant communities of the Pacific Northwest in the United States. It associates with bacteria in the soil to fix nitrogen from the atmosphere, thereby elevating soil nitrogen levels and encouraging the invasion of the community by other nonnative plants like itself. Researchers have used sugar-amendment to decrease soil nitrogen and restore native plant growth. Our study took place in Glacial Heritage Preserve, a Puget lowland prairie that has been invaded by broom in several areas. We aimed to investigate whether sugar-amendment increased or decreased bacterial and fungal abundance in native …

Diversity, Distribution, And Expression Of Diazotroph Nifh Genes In Oxygen-Deficient Waters Of The Arabian Sea, Amal Jayakumar, Mamoon M. D. Al-Rshadat, Bess B. Ward, Margaret R. Mulholland

OES Faculty Publications

The Arabian Sea oxygen minimum zone (OMZ), the largest suboxic region in the world's oceans, is responsible for up to half of the global mesopelagic fixed nitrogen ( N ) loss from the ocean via denitrification and anammox. Dinitrogen (N₂) fixation is usually attributed to cyanobacteria in the surface ocean. Model prediction and physiological inhibition of N₂ fixation by oxygen, however, suggest that N₂ fixation should be enhanced near the oxygen-deficient zone (ODZ) of the Arabian Sea. N₂ fixation and cyanobacterial nifH genes (the gene encoding dinitrogenase reductase) have been reported in surface waters overlying …

Intra And Interhost Dynamics Shaping Arbovirus Adaptation And Evolution, Alexander T. Ciota

Legacy Theses & Dissertations (2009 - 2024)

Arthropod-borne viruses (arboviruses), which are predominately mosquito-borne and almost exclusively RNA viruses, are maintained in nature in complex transmission cycles involving blood sucking invertebrates and vertebrate hosts. Although over 120 arboviruses are human pathogens responsible for causing a significant and expanding global health burden, a detailed understanding of the complex interactions between these pathogens and their hosts, particularly invertebrate hosts, is lacking. Defining these interactions is necessary if we are to understand the selective pressures and, therefore, evolutionary, adaptive, and epidemiological potential of arboviruses. This requires experimental infection and evolution studies, particularly in vivo, with natural hosts. The results presented …

Novel Lineages Of Prochlorococcus And Synechococcus In The Global Oceans, Sijun Huang, Steven W. Wilhelm, H. Rodger Harvey, Karen Taylor, Nianzhi Jiao, Feng Chen

OES Faculty Publications

Picocyanobacteria represented by Prochlorococcus and Synechococcus have an important role in oceanic carbon fixation and nutrient cycling. In this study, we compared the community composition of picocyanobacteria from diverse marine ecosystems ranging from estuary to open oceans, tropical to polar oceans and surface to deep water, based on the sequences of 16S-23S rRNA internal transcribed spacer (ITS). A total of 1339 ITS sequences recovered from 20 samples unveiled diverse and several previously unknown clades of Prochlorococcus and Synechococcus. Six high-light (HL)-adapted Prochlorococcus clades were identified, among which clade HLVI had not been described previously. Prochlorococcus clades HLIII, HLIV and …

Grazing On Synechococcus Spp. By The Red-Tide Dinoflagellate Karenia Brevis: Implications For Bloom Dynamics In The Gulf Of Mexico, Leo A. Procise

OES Theses and Dissertations

Karenia brevis, the toxic dinoflagellate responsible for massive red tides in the Gulf of Mexico (GOM), causes fish kills, shellfish poisoning, and acute respiratory irritation in humans. Bloom initiation and maintenance have been linked to the physical environment as well as various nutrient input mechanisms. To date, efforts to quantify nitrogen (N) sources fueling K. brevis blooms in the GOM have not included mixotrophic grazing although many dinoflagellates, including K. brevis, are known to be capable of mixotrophy. This dissertation reports field and laboratory results demonstrating that natural bloom populations and K. brevisisolates from the West Florida …

Do Larval Traits Re-Evolve? Evidence From The Embryogenesis Of A Direct-Developing Salamander, Plethodon Cinereus, Ryan Kerney, David Blackburn, Hendrik Muller, James Hanken

Ryan Kerney

Recent molecular phylogenies suggest the surprising reacquisition of posthatching metamorphosis within an otherwise directdeveloping clade of lungless salamanders (family Plethodontidae). Metamorphosis was long regarded as plesiomorphic for plethodontids, yet the genus Desmognathus, which primarily includes metamorphosing species, is now nested within a much larger clade of direct-developing species. The extent to which the putative reacquisition of metamorphosis in Desmognathus represents a true evolutionary reversal is contingent upon the extent to which both larva-specific features and metamorphosis were actually lost during the evolution of direct development. In this study we analyze development of the hyobranchial skeleton, which is dramatically remodeled during …

Diversity Of Bacteria And Archaea In Hypersaline Sediment From Death Valley National Park, California, J.-S. Kim, M. Makama, J. Petito, N.-H. Park, Frederick M. Cohan, R. S. Dungan

Frederick M. Cohan

No abstract provided.

The Variable Subdomain Of Escherichia Coli Seca Functions To Regulate Seca Atpase Activity And Adp Release, Sanchaita Das, Lorry M. Grady, Jennifer Michtavy, Yayan Zhou, Frederick M. Cohan, Manju M. Hingorani, Donald B. Oliver

Frederick M. Cohan

No abstract provided.

Diversity Of Bacteria And Archaea In Hypersaline Sediment From Death Valley National Park, California, J.-S. Kim, M. Makama, J. Petito, N.-H. Park, Frederick M. Cohan, R. S. Dungan

Frederick M. Cohan

No abstract provided.

Comment On "Population Genomics Of Early Events In The Ecological Differentiation Of Bacteria", Sarah M. Kopac, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.