Ecology and Evolutionary Biology Commons^™

The Human Oral Microbiome, Floyd E. Dewhirst, Tuste Chen, Jacques Izard, Bruce J. Paster, Anne C. R. Tanner, Wen-Han Yu, Abirami Lakshmanan, William G. Wade

Department of Food Science and Technology: Faculty Publications

The human oral cavity contains a number of different habitats, including the teeth, gingival sulcus, tongue, cheeks, hard and soft palates, and tonsils, which are colonized by bacteria. The oral microbiome comprises over 600 prevalent taxa at the species level, with distinct subsets predominating at different habitats. The oral microbiome has been extensively characterized by cultivation and culture-independent molecular methods such as 16S rRNA cloning. Unfortunately, the vast majority of unnamed oral taxa are referenced by clone numbers or 16S rRNA GenBank accession numbers, often without taxonomic anchors. The first aim of this research was to collect 16S rRNA gene …

Access To Mutualistic Endosymbiotic Microbes: An Underappreciated Benefit Of Group Living, Michael Lombardo

Michael P Lombardo

"The original publication is available at www.springerlink.com"

A central question in behavioral ecology has been why animals live in groups. Previous theories about the evolution of sociality focused on the potential benefits of decreased risk of predation, increased foraging or feeding efficiency, and mutual aid in defending resources and/or rearing offspring. This paper argues that access to mutualistic endosymbiotic microbes is an underappreciated benefit of group living and sets out to reinvigorate Troyer’s hypothesis that the need to obtain cellulolytic microbes from conspecifics influenced the evolution of social behavior in herbivores and to extend it to nonherbivores. This extension is …

On The Evolution Of Sexually Transmitted Diseases In Birds, Michael Lombardo

Michael P Lombardo

"The definitive version is available at www.blackwell-synergy.com".

Sexually transmitted diseases (STDs) in animals are caused by pathogens that are transmitted during copulation. Birds have played an important role in the development of STD-centered theories of mating behavior. However, it is not known whether STDs exist in wild bird populations. While the avian cloaca with its dual functions of gamete transfer and excretion seemingly predisposes birds for the evolution of STDs, the life history patterns of most birds (i.e., seasonal breeders with relatively brief annual periods of sexual activity) suggest otherwise. The importance of STDs as selective forces that shape host …

Ecology And Relationships Of Rhabdias Spp. (Nematoda: Rhabdiasidae) From North American Amphibians And Reptiles, Gabriel J. Langford

School of Biological Sciences: Dissertations, Theses, and Student Research

Lungworms of the cosmopolitan genus Rhabdias (Nematoda: Rhabdiasidae) are among the most common parasites of amphibians and squamate reptiles. The life cycles, life histories, host specificities, and evolutionary relationships of Rhabdias spp. were studied through examination of their free-living and parasitic stages in amphibians and reptiles. This study found free-living development of anuran lungworms was primarily limited to heterogonic reproduction, whereas snake lungworms primarily reproduced homogonically. Infective anuran lungworms penetrated the skin of frogs and toads; in contrast, snake lungworms penetrated snake esophageal tissue during per os infections. Our molecular phylogeny strongly supported separate clades for anuran and snake lungworms, …

Antimicrobial And Antioxidant Activities Of Essential Oil And Methanol Extract Of Jasminum Sambac From Djibouti, Fatou Abdoul-Latif, Prosper Edou, François Eba, Nabil Mohamed, Adwa Ali, Samatar Djama, Louis-Clément Obame, Mamoudou Hama Dicko

Pr. Mamoudou H. DICKO, PhD

The essential oil of jasminum sambac from Djibouti was subjected to screening for their possible antioxidant activity by two complementary test systems, namely DPPH free radical scavenging and beta-carotene-linoleic acid assays. Butylated hydroxytoluene (BHT) was used as positive control in both test systems. In the DPPH test system, the IC50 value of essential oil and methanol extract were respectively 7.43 and 2.30 μg/ml. In the beta-carotene-linoleic acid system, oxidation was effectively inhibited by Jasminum sambac, the RAA value of essential oil and methanol extract were respectively 96.6 and 93.9%. When compared to BHT, the essential oil and methanol extract had …

Management Strategies To Improve Yield And Nitrogen Use Of Spring Wheat And Field Pea In The Semi-Arid Northern Great Plains Usa, Andrew W. Lenssen, Brett Allen, Upendra Sainju, Thecan Caesar, Robert Lartey, Robert Evans

Andrew W. Lenssen

Available water and N fertility are primary constraints to crop production in the northern Great Plains of the USA. A field trial was initiated in 2004 to compare four crop rotations in a complete factorial of two tillage and two management systems. Rotations were continuous spring wheat (SW), pea-SW, barley hay-pea-SW, and barley hay-corn-pea-SW. Tillage systems were no till and field cultivator tillage, while management systems were conventional and ecological. Conventional management included broadcast nitrogen fertilizer, standard seeding rates, and short stubble height. Ecological management practices varied by crop, and included banded nitrogen fertilizer for cereals, increased seeding rate, delayed …

Early Cranial Patterning In The Direct-Developing Frog Eleutherodactylus Coqui Revealed Through Gene Expression, Ryan Kerney, Joshua Gross, James Hanken

Ryan Kerney

Genetic and developmental alterations associated with the evolution of amphibian direct development remain largely unexplored. Specifically, little is known of the underlying expression of skeletal regulatory genes, which may reveal early modifications to cranial ontogeny in direct-developing species. We describe expression patterns of three key skeletal regulators (runx2, sox9, and bmp4) along with the cartilage-dominant collagen 2a1 gene (col2a1) during cranial development in the direct- developing anuran, Eleutherodactylus coqui. Expression patterns of these regulators reveal transient skeletogenic anlagen that correspond to larval cartilages, but which never fully form in E. coqui. Suprarostral anlagen in the frontonasal processes are detected through …

Synthetic Genome: Now That We’Re Creators, What Should We Create?, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.

The Ecology Of Speciation In Bacillus, Nora Connor, Johannes Sikorski, Alejandro P. Rooney, Sarah Kopac, Alexander F. Koeppel, Andrew Burger, Scott G. Cole, Elizabeth B. Perry, Danny Krizanc, Nicholas C. Field, Michele Slaton, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.

Regulatory Elements Of Xenopus Col2a1 Drive Cartilaginous Gene Expression In Transgenic Frogs, Ryan Kerney, Brian K. Hall, James Hanken

Ryan Kerney

This study characterizes regulatory elements of collagen 2α1 (col2a1) in Xenopus that enable transgene expression in cartilage-forming chondrocytes. The reporters described in this study drive strong cartilage-specific gene expression, which will be a valuable tool for further investigations of Xenopus skeletal development. While endogenous col2a1 mRNA is expressed in many embryonic tissues, its expression becomes restricted to tadpole and adult chondrocytes. This chondrocyte-specific expression is recapitulated by col2a1 reporter constructs, which were tested through I-SceI meganuclease-mediated transgenesis. These constructs contain a portion of the Xenopus tropicalis col2a1 intron, which aligns to a cartilage-specific intronic enhancer that has been well characterized …