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Full-Text Articles in Ecology and Evolutionary Biology

Insights To Protein Pathogenicity From The Lens Of Protein Evolution, Janelle Nunez-Castilla Jun 2022

Insights To Protein Pathogenicity From The Lens Of Protein Evolution, Janelle Nunez-Castilla

FIU Electronic Theses and Dissertations

As protein sequences evolve, differences in selective constraints may lead to outcomes ranging from sequence conservation to structural and functional divergence. Evolutionary protein family analysis can illuminate which protein regions are likely to diverge or remain conserved in sequence, structure, and function. Moreover, nonsynonymous mutations in pathogens may result in the emergence of protein regions that affect the behavior of pathogenic proteins within a host and host response. I aimed to gain insight on pathogenic proteins from cancer and viruses using an evolutionary perspective. First, I examined p53, a conformationally flexible, multifunctional protein mutated in ~50% of human cancers. Multifunctional …


Growth Of Diatom Fistulifera Alcalina In Bacterial Co-Culture And Comparative Mitogenomics Of Fistulifera Species, Erwin David Berthold Mar 2021

Growth Of Diatom Fistulifera Alcalina In Bacterial Co-Culture And Comparative Mitogenomics Of Fistulifera Species, Erwin David Berthold

FIU Electronic Theses and Dissertations

Diatoms are excellent biological models of growth and intracellular oil generation. The productivity and compounds of diatoms, especially oils, support aquatic food chains and human medical and industrial needs. The qualities that made diatoms prolific producers, specifically diatom physiological features such as growth rates with intracellular lipid storage in alkaline environments, are however poorly understood. Another physiological aspect that remains unexplored is the effects of bacteria on the growth and lipid production of alkaliphilic diatoms. More studies, especially co-cultures, are needed for advances in diatom biology and strain performance for the algal biotechnological field. Besides physiology, diatom genetics using next-generation …


Changes In Soil Microbial Functioning In Coastal Wetlands Exposed To Environmental Stressors And Subsidies, Shelby M. Servais May 2018

Changes In Soil Microbial Functioning In Coastal Wetlands Exposed To Environmental Stressors And Subsidies, Shelby M. Servais

FIU Electronic Theses and Dissertations

Environmental perturbations are ubiquitous features of ecosystems and shape ecological structure and function. Climate change will alter the intensity and frequency of disturbances and expose ecosystems to novel combinations of useful inputs (subsidies) and harmful inputs (stressors). Coastal wetlands are particularly vulnerable to changing environmental conditions and are increasingly exposed to effects of interacting subsidies and stressors. In particular, the Florida Coastal Everglades, which has experienced accelerated change due to a history of water management practices, is vulnerable to new disturbances associated with climate change. The low-lying Florida Everglades faces multiple disturbances from storm surge, nutrient enrichment, and sea-level rise …


Phenomenological And Molecular Basis Of The Cnidarian Immune System, Tanya Brown Jun 2017

Phenomenological And Molecular Basis Of The Cnidarian Immune System, Tanya Brown

FIU Electronic Theses and Dissertations

Coral reefs are one of the most diverse ecosystems on the planet due partially to the habitat structure provided by corals. Corals are long lived organisms that can live for hundreds of years and as a result growth of many species is very slow. As a result of this, recovery of corals from disease outbreaks is very slow and difficult and therefore the ecosystem is deteriorating rapidly. Due to this increase in disease and its detrimental effect on coral reefs, it has become imperative to study how corals respond to disease outbreaks. The response of the coral to pathogens is …


Screening Of Plants For Antibacterial Properties: Growth Inhibition Of Staphylococcus Aureus By Artemisia Tridentata, Steven Ross Eichelbaum Nov 2016

Screening Of Plants For Antibacterial Properties: Growth Inhibition Of Staphylococcus Aureus By Artemisia Tridentata, Steven Ross Eichelbaum

FIU Electronic Theses and Dissertations

Drug-resistant pathogenic and opportunistic bacteria are increasing in occurrence and prevalence, and pose a dangerous threat to human health. In the search for novel antibiotics with which to combat this threat, plants, specifically those used in traditional medicine with ascribed antibacterial properties, offer a promising and potentially vast source of such therapeutic compounds. The purpose of this study was therefore to screen chemical extracts created from various plant species for antibacterial properties versus pathogenic bacterial species. In the course of these antibacterial assays, we successfully identified a methanol extract derived from Artemisia tridentata tridentata plant material as capable of inhibiting …


Controls On Benthic Microbial Community Structure And Assembly In A Karstic Coastal Wetland, Nicholas O. Schulte Mar 2016

Controls On Benthic Microbial Community Structure And Assembly In A Karstic Coastal Wetland, Nicholas O. Schulte

FIU Electronic Theses and Dissertations

The assembly mechanisms underlying microbial community abundance, biotic interactions, and diversity over space and time are unresolved, particularly in benthic microbial mats distributed along environmental gradients. Experimental enrichment of nutrient-limited microbial mats from the Florida Everglades along a nutrient subsidy-salinity stress gradient stimulated autotrophic and heterotrophic metabolism, growth, and diversity independent of autotroph-heterotroph interactions across treatments and space. These results suggest spatial segregation of autotrophic and heterotrophic components within mats. Considering only the diatom component of Everglades mats over space and time, the subsidy-stress gradient controlled diatom compositional turnover at broad spatial scales while environmental and dispersal-based processes structured diatom …


Discovery & Born-Digital Archiving: Open Source Systems For Preservation And Access, L. Bryan Cooper, Margarita Perez-Martinez Dec 2015

Discovery & Born-Digital Archiving: Open Source Systems For Preservation And Access, L. Bryan Cooper, Margarita Perez-Martinez

Works of the FIU Libraries

The Everglades Explorer (EE) portal at http://ee.fiu.edu continues to evolve with the addition of the Internet Archive's Archive-It, and future planned alignment with the Digital Public Library of America (DPLA). The reasons for the development of the portal continue to exist, as originally perceived four years ago. Adaptations to ongoing change and system testing continues, and the use of Archive-It has broad institutional potential beyond EE. Cross-walking skills continue to grown, and will benefit future syndication and discovery system integration. The metadata normalization and harmonization will help save time for the end-user. Preservation and access to learning and research …


Environmental Influences On Bacterio-Phytoplanktonic Coupling And Bacterial Growth Efficiency In A Sub-Tropical Estuary, Rachel Kotkowski Apr 2014

Environmental Influences On Bacterio-Phytoplanktonic Coupling And Bacterial Growth Efficiency In A Sub-Tropical Estuary, Rachel Kotkowski

FIU Electronic Theses and Dissertations

Bacterio-phytoplanktonic coupling and bacterial growth efficiency (BGE) measurements were used to analyze microbial trophic dynamics and the influence of environmental factors in Florida Bay, Florida. Phytoplankton gross primary productivity (GPP) was measured using 24-hour in situ oxygen incubations; bacterial productivity (BP) was measured using 3H- thymidine incorporation. Weak bacterio-phytoplanktonic coupling was observed over the sampling period. BP was more influenced by local total nitrogen concentrations while GPP was more evenly distributed. BGE rates were low but consistent with marine and estuarine ecosystems worldwide. Results suggest that bacterioplankton growth in Florida Bay is relatively uncoupled from phytoplankton production, which may …


Characterization Of Rhizobial Diversity And Relationship Of Rhizobial Partner And Legume Performance In Four South Florida Pine Rockland Soils, Vanessa Sánchez Mar 2014

Characterization Of Rhizobial Diversity And Relationship Of Rhizobial Partner And Legume Performance In Four South Florida Pine Rockland Soils, Vanessa Sánchez

FIU Electronic Theses and Dissertations

Pine rocklands are endangered ecosystems unique to south Florida, the Bahamas and Cuba. As a result of their karstic calcium carbonate­rich soil, these systems are limited in phosphorus and nitrogen, making symbiotic associations critical to plant growth. Four leguminous species (Cajanus cajan, Chamaecrista fasciculata, Tephrosia angustissima, and Abrus precatorious) were used to determine the relationship between rhizobial partners and plant performance, and the symbiosis related gene nifH was amplified to characterize the diversity of rhizobial symbionts. Plants were grown in soils from four different south Florida pine rocklands, and a salinity treatment was added to determine …