Ecology and Evolutionary Biology Commons^™

Factors Associated With Acoustic Bat Presence During Spring Emergence In The Appalachian Mountains Of Western Virginia, Emily Kirk Pody

Theses, Dissertations and Capstones

Studies of threats that bats face during hibernation have increased in response to white-nose syndrome (WNS), a fungal disease that has ravaged North American bat populations. However, impacts of WNS on bat ecology during spring emergence, when bats are potentially recovering from infection and allocating resources for reproduction, is relatively understudied. As more bat species become listed at the federal and state level, the need to understand the factors associated with spring emergence is critical for improving conservation guidelines and habitat management practices. Acoustic monitoring is an efficient method for monitoring bat presence for prolonged periods of time, giving biologists …

The Comparison Of Different Wetland Fish Assemblages Over Time, Robert Edward Adelstein

Theses, Dissertations and Capstones

Wetlands provide essential ecosystem services. Historically, we have drained and filled 73% of wetlands for agricultural use throughout the United States from the 1780s to the 1980s (Dahl, 1990). A nationwide focus on restoring wetlands has since occurred. Literature on restored/mitigated wetlands is rife with examples that do and do not support the same ecosystem services as natural wetlands (Langston, 1997; Meil, 2014). Restoration of wetlands occurred at the Green Bottom Wildlife Management Area (GBWMA) over several decades. Various sections of the wetland were classified by age, water depth, and vegetation. One hypothesis was that differences in fish assemblage would …

Assessing The Unionid Assemblage Of The Robert C. Byrd Pool, Ohio River, Ethan Hunter Bellamy

Theses, Dissertations and Capstones

Unionid mussels are the most imperiled taxa in the United States and are vastly understudied. The entire Robert C. Byrd (RCB) pool of the Ohio River is the study area. I selected sites from the 2019 and 2013 ORSANCO RCB pool assessments that utilize random site selection across the pool. I used SCUBA to survey and collect data on unionid diversity, reproduction, and habitat. I collected 1,083 individuals over 19 species in RCB pool. I compared results from RCB pool survey to two similar surveys conducted in Greenup Pool. Both richness and abundance in the Upper Section of Greenup Pool …

Crippling Rapid Evolution Of Metastasis And Drug Resistance In A549 Non-Small Cell Lung Cancer Cells With The Clinically Relevant Hsp90 Inhibitor Auy922, Nickolas Anthony Bacon

Theses, Dissertations and Capstones

The ability for species to evolve new features in response to changing circumstances in order to survive and propagate is a ubiquitous observation on both the macroscopic and microscopic levels of living systems. It should be no surprise, then, that diseases such as cancer utilize their own forms of adaptation to perpetuate themselves when exposed to external threats. Indeed, concepts drawn from Darwinian evolution are now widely accepted to help explain certain aspects of carcinogenesis and malignant progression, the sum of which have come to be known as the theory of tumor evolution. Since metastasis and drug resistance are features …

Using Environmental Dna And Occupancy Modeling To Examine Drivers Of Eastern Hellbender Extirpation And Sampling Method Efficiency In West Virginia, Sean Michael Wineland

Theses, Dissertations and Capstones

Amphibian population declines and range constrictions are widespread but poorly understood. Effective conservation planning relies on accurate distribution data to develop a fundamental understanding of causal changes in species distributions. However, conventional detection methods for cryptic and elusive amphibians suffer from imperfect detection. Environmental DNA (eDNA) has emerged as an innovative and powerful conservation tool for detecting aquatic species presence; however comparative studies aimed at fully understanding eDNA detection probabilities are lacking. In this study, I used environmental DNA (eDNA) sampling methods and detailed historical records to identify drivers of extirpation and sampling method efficiency for an imperiled, long-lived giant …

A Characterization Of West Virginia Coyotes (Canis Latrans) Utilizing Skull Morphology, Katharina E. Scholer

Theses, Dissertations and Capstones

Coyotes (Canis latrans) are now found throughout North and Central America, but before European colonization were restricted to west of the Mississippi. Migration occurred in two major paths to the East; north over the Great Lakes (through Canada) and south below the Great Lakes. The location of these routes is significant because those migrating north interbred with the wolves that reside there. These hybrid animals are larger and behaviorally different from their western counterparts. It is possible to differentiate these hybrids morphologically and genetically. Hybrids are known to be located in Maine, New York, and Pennsylvania, but the interest of …

Establishing Environmental Variability In The Toxins Produced By A Single Strain Of Prymnesium Parvum, Amy Parsons-White

Theses, Dissertations and Capstones

Prymnesium parvum (P. parvum) is a toxin producing haptophyte that has spread globally over the past 100 years. The algae, initially a marine organism, has moved inland to less saline waters. In addition, there is variability in the efficacy of the toxins produced from bloom to bloom. Variation between strains has been suggested as the reason for bloom differences; however, environmental variability within a single strain must first be established. Here, a single strain of P. parvum was cultured at three environmentally relevant salinities, 6psu, 13psu, and 35psu. Three different environmental shocks, decreased temperature, raised pH, and addition of specific …

Use Of An Artificial Pond And Marshes By Amphibians And Reptiles In West Virginia, Vanessa A. Dozeman

Theses, Dissertations and Capstones

A farm pond approximately 40 years old located in Wayne County, West Virginia was studied to determine the ingress and egress of amphibians and reptiles. In addition to the pond, 2 small marshes were also examined. The study extended from February 2003 to November 2003. The pond is approximately 36 m long and 38 m wide and is located on a south-facing hillside at 202 m in elevation. A drift fence composed of landscaping cloth was constructed to completely encircle the site. At every 5.15 m, funnel traps were positioned on both sides of the fence. Traps were checked daily …

Factors Influencing Spatial Variability In Soil Nitrogen (N) Dynamics In N-Treated And Untreated Watersheds Of The Fernow Experimental Forest, West Virginia, Nikki Lenore Lyttle

Theses, Dissertations and Capstones

The central Appalachian region of the United States receives some of the highest inputs of nitrogen (N) due to acidic deposition in the nation. It is believed that these high could levels contribute to a decline in forest soils within the next 50 to 70 yrs. This study examines factors that influence spatial variability in N-treated and untreated watersheds of the Fernow Experimental Forest, Parsons, West Virginia. Within each of the two watersheds [WS4 untreated control, > 100 yr.; WS3 N-treated, acidified, clear cut, ~ 31 yr.], two 0.04 ha plots, one high N and one low N, were selected for …

The Life History Of The Cave Salamander, Eurycea Lucifuga Rafinesque, In West Virginia, Andrew James Longenecker

Theses, Dissertations and Capstones

In West Virginia, as throughout its range, there is limited information about the life history of the cave salamander (Eurycea lucifuga). The purpose of this study was to describe the natural history of this species in West Virginia. The objectives of this study were to determine habitat selection of the cave salamander, to ascertain its environmental characteristics, and to study its reproductive and non-reproductive biology. Three study caves were located in Greenbrier County, West Virginia. All sites were examined at least once each month from May 1999 through April 2000. Caves were divided into three zones: entrance, twilight, …

Ecology And Sympatric Relations Of Crevice Salamanders In Randolph County, West Virginia, Jayme Linn Waldron

Theses, Dissertations and Capstones

By implementing a mark-recapture study, I investigated the ecology of three species of sympatric plethodontid salamanders (Aneides aeneus, Desmognathus ochrophaeus, and Plethodon glutinosus) on rock outcrops in the Westvaco Wildlife and Ecosystem Research Forest, Randolph County, West Virginia. By examining activity patterns, vertical stratification, and habitat preference for A. aeneus, D. ochrophaeus, and P. glutinosus, I attempted to offer some insight into how these sympatric species avoid competition on small, isolated rock outcrops. Twenty-one surveys, both nocturnal and diurnal, were performed between 11 May and 17 October, 1999. During this time, 89 salamanders, encompassing five species ( …

Changes Among Ohio River Fish Populations Due To Water Quality Improvements And High-Lift Dams, Benjamin Lowman

Theses, Dissertations and Capstones

Fish populations in the Ohio River have been monitored extensively by the Ohio River Valley Water Sanitation Commission (ORSANCO), along with state and Federal agencies, for over 40 years. The population data, collected via lock chamber rotenone surveys, showed that many species of Ohio River fish have demonstrated marked increases in abundance since these studies began. These trends in population density, both gradual and sharp, are likely associated with water quality improvements and the construction of high-lift dams. Pearson-r correlation through linear regression analyses showed 23 species or 42% of populations examined exhibit significant density increases from 1957 to 1998. …

The Relationship Between Ohio River Bass Tournament Success Rates, Forage Fish Abundance And Environmental Conditions, Jeffrey V. Ginger

Theses, Dissertations and Capstones

Due to increasing pressure from Ohio River bass anglers with regard to decreasing catch rates in 1981,1990, and 1997, the West Virginia Division of Natural Resources (WVDNR) initiated a study of black bass populations. The Ohio River Valley Water Sanitation Commission (ORSANCO), in conjunction with state and Federal agencies, has collected population data on Ohio River fishes at lock chambers and has measured various water quality parameters since 1957. That data coupled with Ohio River bass tournament data collected by the DNR since 1975 and other fishery data sets provided a historic look into the variability of angler success. These …

Benthic Biodiversity And Physico-Chemical Parameters Of Acid Mine Drainage, Acid Impacted And Nonimpacted Streams, Stephen D. Greene

Theses, Dissertations and Capstones

South Fork of Sand Lick Creek, Logan County, West Virginia, drains an abandoned coal strip mine which had exploited Pottsville series coalbeds (Pennsylvania strata). These strata outcrop throughout southwestern West Virginia. North Fork watershed is relatively unchanged, save a small roadcut throughout. South Fork benthic community had not recovered although mining activity had ceased about 20 years earlier. Benthic communities were analyzed with detrended correspondence analysis (DCA). Family Chironomidae predominated South Fork benthic community throughout the study. North Fork’s benthic community had as major contributors acid resistant caddisfly family Hydropsychidae, mayfly family Baetidae, and stonefly families Perlodidae and Nemouridae. Family …

Food Habits Of Salmo Trutta And Salvelinus Fontinalis (Pisces: Salmonidae) In Relation To Seasonal Changes And Mitigative Liming Effects In Dogway Fork Of The Cranberry River, West Virginia., Jason A. Morgan

Theses, Dissertations and Capstones

Dogway Fork, a tributary of the Cranberry River in West Virginia, is acidic due to acid precipitation and poorly buffered soils. This study is part of a long-term investigation involving the effects of continuous limestone neutralization on the fishes and benthos of an acid stream. Prior to treatment in 1988, the stream had a pH of 4.5 and there were no fish present. Since treatment began, the pH of the stream has risen to 6.8. Nine species of fish have been collected and six species have reproduced following the improvement in water quality. Densities of specific acid-sensitive benthic macroinvertebrates have …

Distribution Of The Sucker Family (Pisces: Catostomidae) In The Ohio River, Tara L. Rose

Theses, Dissertations and Capstones

The family Catostomidae represents a major component of the fish fauna in the Ohio River. Because of their abundance, large biomass, and susceptibility to environmentally-induced anomalies, suckers are expected to significantly influence the Ohio River Fish Index (ORFIn), a biological index being developed by the Ohio River Valley Water Sanitation Commission (ORSANCO) for large river systems. To correctly interpret information obtained from ORFIn, the impact of suckers upon the index must be thoroughly understood, including relative importance and uneven spatial distribution of Ohio River suckers. In this study, suckers represented 7.1 percent of the total fish captured and 36.6 percent …

Ecology And Natural History Of Rana Clamitans Melanota In West Virginia, Alison M. Rogers

Theses, Dissertations and Capstones

Fourteen study sites were sampled with aquatic funnel traps and D-frame net sweeps in the plateau and mountain region of West Virginia to determine differences in larval period. Larval stage class abundance and larval total length and mass measurements were recorded from February to December 1997. Larval stage classes 25 and 26, the first free living stages, were absent from the plateau region from February to April, but were present in the mountain region. Pre-transformation larval stages, 40 through 44, were found from May through October, and peaked in July. Significant relationships were found with simple linear regression analysis between …

Ecology And Natural History Of The Cow Knob Salamander, Plethodon Punctatus Highton, In West Virginia, Robert B. Tucker

Theses, Dissertations and Capstones

Very little has been written on the ecology and natural history of the Cow Knob salamander, Plethodon punctatus Highton, since it was described by Highton (1971). Plethodon punctatus is known only from the higher elevations of Great North (above 2,800 ft) and Shenandoah (above 3,000 ft.) Mountains in Virginia and West Virginia (Highton, 1972; Conant and Collins, 1991). The type locality is Cow Knob, Pendleton County, West Virginia. Plethodon punctatus is a large (up to 75 mm snout-to-vent length) dorso-ventrally flattened salamander with creamish iridiophore spots on the back and sides, webbed feet for a fossorial lifestyle, and large …

The Use Of Isoelectric Focusing To Assess The Genetic Diversity Of The Cyprinid Fishes: Rhinichthys Cataractae And Rhinichthys Atratulus In West Virginia, Fauzia Alam Miah

Theses, Dissertations and Capstones

In this study, isoelectric focusing is used to separate proteins of Rhinichthys cataractae and R. atratulus and conventional staining is used to identify alleles of six genes. The objective of this work is to determine whether these methods can effectively assay isozymes that may be used to establish the relationship between anthropogenic stress and genetic diversity. Alleles for LDH-A, LDH-B, EST-A, EST-B, EST-C, and EST-D loci were identified for two species of fish, R. atratulus and R. cataractae. Both fish species are known to be sensitive to anthropogenic stress and known to be widely distributed in stressed environments. Alleles …

Life History, Ecology, And Low Ph Tolerance Of The Freshwater Prawn, Palaemonetes Kadiakensis Rathbun, In A Mitigated Wetland Of The Green Bottom Wildlife Management Area, West Virginia, Jennifer Ann Aderman

Theses, Dissertations and Capstones

An ecological life history study of the freshwater prawn Palaemonetes kadiakensis was conducted at the mitigated wetland of the Green Bottom Wildlife management Area (GBWMA). Monthly samples were collected from November 1996 through November 1997. In addition, weekly samples were collected from May through November for growth and reproductive analysis. Monthly samples yielded 520 prawns. Palaemonetes kadiakensis had a one-year life cycle, with all adults dying by September. Males averaged 23.82 mm (range = 14.15 to 36.75 mm) and females averaged 26.78mm (range = 15.06 to 38.60 mm) in length. Males averaged 0.087g (range = 0.014to 0.293 g) and females …

The Use Of Emergent Rocks As Refugia For The Cheat Mountain Salamander, Plethodon Nettingi Green, Beth Anne Pauley

Theses, Dissertations and Capstones

Plethodon nettinqi was listed as a threatened species in 1989 by the United States Fish and Wildlife Service (Pauley, 1991). Its total range is within 5 counties in eastern West Virginia. There are fewer then 60 disjunct populations known and most populations are above 3,000 ft. and are associated with emergent rocks or narrow ravines with Rhododendron. It is hypothesized the P. netting! survived lumbering practices at the turn of the century by taking refuge beneath large emergent rocks and narrow ravines with Rhododendron. This study examined environmental factors associated with emergent rocks that might regulate the distribution of P. …

Observations On Reproduction, Isozyme Patterns, And Meristics In Mosquitofish (Pisces: Poeciliidae) From West Virginia, Matt Mcreynolds

Theses, Dissertations and Capstones

Prior to 1988, two subspecies of the mosquitofish Gambusia affinis (G. a. affinis and G. a. holbrooki) were recognized along the Gulf and Atlantic Coasts of the United States. Based on electrophoretic analysis, subsequent investigators recognized G. holbrooki and G. affinis as separate species. Populations in drainages west of Mobile Bay were considered G. affinis, and those east of this divide being G. holbrooki. The only mosquitofish population in West Virginia is found in the Meadow River wetlands (1392 ha). This wetland complex is located at the western end of Greenbrier County in the Allegheny Mountain Province. …

Nitrogen Transformations In A Mitigated Wetland In The Green Bottom Wildlife Management Area, Mark Allen Fisher

Theses, Dissertations and Capstones

Soil nitrogen (N) dynamics have been shown to be quite sensitive to the soil hydrology. Creation of a mitigation freshwater wetland from an old field provided an opportunity to examine changes in N dynamics in terrestrial, aquatic (but formerly terrestrial), and transition (seasonally inundated) soils.

This thesis determined N availability using in situ incubations of soils taken from three distinct habitat conditions based on the degree of inundation following wetland creation: (1) old field (no inundation), (2) transition (seasonally inundated, initially beneath 10-20 cm of water), and (3) mitigation wetland (permanently inundated). Sample plots were located along five parallel transects …

Geographic Variation Of S̲O̲R̲E̲X̲ C̲I̲N̲E̲R̲E̲U̲S̲ In West Virginia, Lisa Jane Gatens

Theses, Dissertations and Capstones

The morphological and geographical diversity of Sorex cinereus is evident in West Virginia. In an attempt to identify patterns of morphological variation relative to age, sex, and geographic locality, and to clarify taxonomic status of the West Virginia soricids by defining diagnostic characters applicable to this area, a sample of 288 specimens representing three taxa were analyzed statistically. Standard external measurements were recorded, and a series of 12 cranial and dental characters were measured and recorded for each specimen. Morphological variation due to age was found in S. c. cinereus, but not in the smaller reference samples of S. …

Food Habits Of Largemouth Bass, Micropterus Salmoides (Lacepede) And Spotted Bass, Micropterus Punctulatus (Rafinesque), From Beech Fork Reservoir, A New Impoundment In Wayne And Cabell Counties, West Virginia, Michael A. Arcuri

Theses, Dissertations and Capstones

A study was undertaken to determine the food habits of two game fishes, the largemouth bass Micropterus salmoides (Lacepede) and the spotted bass M. punctulatus (Rafinesque) in Beech Fork Reservoir shortly after its impoundment. A total of 171 largemouth bass and 95 spotted bass were collected from July 1978, through June 1979, and their stomach contents analyzed.

Largemouth bass fed primarily on fishes, particularly young-of-the-year bluegill. Aquatic and terrestrial invertebrates were also important food items, especially during the spring and fall . The dominant aquatic forms were chironomid larvae and pupae while the dominant terrestrial forms were adult dipterans.

Spotted …