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The Regulation Of Glucose Transport In Cultured Vascular Smooth Muscle Cells By Angiotensin Ii And Glucose, Leslie Ann Quinn
The Regulation Of Glucose Transport In Cultured Vascular Smooth Muscle Cells By Angiotensin Ii And Glucose, Leslie Ann Quinn
Theses, Dissertations and Capstones
Glucose transport was assessed in vascular smooth muscle (VSM) cells by measuring the uptake of a radiolabeled non-metabolizable glucose analog, [3H]-2-deoxglucose. VSM cells, isolated from rat aortae by enzymatic digestion, were maintained in culture in Dulbecco's Modified Eagle Medium supplemented with 10% newborn calf serum at 37°C with 5% CO2 and air. Angiotensin II (AU) increased glucose transport by 84%. Significant stimulation occurred by two hours of exposure with the maximum effect being observed between six and eight hours. All effects were concentration dependent with a threshold response being detected at 0.1 nM. All-stimulated transport was blocked by an AU …