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Inhibition Of Voltage-Gated Na+ Current By Nanosecond Pulsed Electric Field (Nspef) Is Not Mediated By Na+ Influx Or Ca²+ Signaling, Vasyl Nesin, Andrei G. Pakhomov

Bioelectrics Publications

In earlier studies, we found that permeabilization of mammalian cells with nsPEF was accompanied by prolonged inhibition of voltage-gated (VG) currents through the plasma membrane. This study explored if the inhibition of VG Na⁺ current (I_Na) resulted from (i) reduction of the transmembrane Na⁺ gradient due to its influx via nsPEF-opened pores, and/or (ii) downregulation of the VG channels by a Ca²⁺ -dependent mechanism. We found that a single 300?ns electric pulse at 1.65.3?kV/cm triggered sustained Na⁺ influx in exposed NG108 cells and in primary chromaffin cells, as detected by increased fluorescence of a …