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Articles 1 - 4 of 4
Full-Text Articles in Cell and Developmental Biology
Creating An In-Vitro Co-Culture Model Of Human Megakaryocytic-Erythroid Progenitors And Endothelial Cells, Clerin John
Creating An In-Vitro Co-Culture Model Of Human Megakaryocytic-Erythroid Progenitors And Endothelial Cells, Clerin John
Holster Scholar Projects
Previous studies have suggested that endothelial cells promote red blood cell (RBC) production in human bone marrow, which has implications for improved treatments for anemia, cancer, and reproduction. However, investigation of this phenomenon is hindered by a dearth of studies that have successfully isolated primary endothelial cells from human bone marrow. Using flow cytometry, magnetic cell separation, and primary cell culture techniques we tested the hypothesis that adult bone marrow-derived endothelial cells can be isolated from previously frozen bone marrow samples by selecting for CD144+ expressing cells and culturing them in vitro. Magnetic cell separation utilizing the marker CD144 to …
Analysis Of Tri-Lineage Collagen Reporter Mice Growth Plates Through Light Sheet Microscopy, Olivia Ferrigno
Analysis Of Tri-Lineage Collagen Reporter Mice Growth Plates Through Light Sheet Microscopy, Olivia Ferrigno
Holster Scholar Projects
The growth plate is an active area of cartilage in the long bones of children. Its main function is to enable limb lengthening, but is a relatively weak structure that is prone to fractures and injuries. These injuries can have severe long term ramifications, as they can create premature bone fusion, also known as a bony bridge, that causes stunted or crooked growth. Dr. Liisa Kuhn’s Lab has developed an accurate, reproducible animal model that mimics this same growth plate behavior and can be used to further analyze the growth plate structure, healing process, and new treatments. This animal model …
Determining The Primary Dna Substrates Of Shld2'S Ob-Fold Domains, Hari Patchigolla
Determining The Primary Dna Substrates Of Shld2'S Ob-Fold Domains, Hari Patchigolla
Holster Scholar Projects
Failure to repair DNA double-stranded breaks leads to cell death. Radiation therapy is commonly used to kill cancer cells by inducing these breaks. However resistance to radiation therapy, due to a hyperactive DNA double-stranded break repair pathway, is a common occurrence that makes cancer patients more prone to relapse. The Shieldin complex is shown to promote DNA-double stranded break repair by binding to DNA at sites of damage. Thus, the objective of this project is to understand the affinity and type of DNA that Shieldin binds to, through gel-shift assays, for the eventual creation of an inhibitor for this protein …
Dietary Analysis And Epigenetic Comparisons Of Drosphila Melanogaster Through Multiple Generations, Xu (Kevin) F. Zheng
Dietary Analysis And Epigenetic Comparisons Of Drosphila Melanogaster Through Multiple Generations, Xu (Kevin) F. Zheng
Holster Scholar Projects
Nutrition and the lack thereof has been a demanding issue in both the undeveloped and developed country. The unbalance diet between healthy and unhealthy diet has led to many of the problems presented in society such as obesity, heart disorders, and loss of longevity. It is now known that calorie may not provide the most accurate information in terms of the lifestyle and the well-being of humans. Rather the composition of the calorie is what becomes integral in people's understanding of the effect of food physiologically. With this knowledge, we seek to use a Drosophila melanogaster as a model organism …