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Articles 1 - 5 of 5
Full-Text Articles in Biotechnology
In Vitro Plant Regeneration, Antioxidant And Antibacterial Studies On Broccoli, Brassica Oleracea Var. Italica, Rosna Binti Mat Taha
In Vitro Plant Regeneration, Antioxidant And Antibacterial Studies On Broccoli, Brassica Oleracea Var. Italica, Rosna Binti Mat Taha
Rosna Binti Mat Taha
Leaf and shoot tip explant of Brassica oleracea var. italica were inoculated on Murashige and Skoog (MS) basal medium supplemented with various concentrations of (BAP) and (IBA), alone and in combinations to achieve plant regeneration. Subsequently, antioxidant and antibacterial activities were determined from In vitro and In vivo plant parts. The highest mean numbers of shoot was observed on combinations of 1 mg l(-1) and 1.5 mg l(-1) of BAP and IBA, 9.49 and 8.69, respectively. The highest number of shoot produced (11.55) per shoot tip explant was recorded on 2.5 mg l(-1) BAP. In addition, highest percentage of roots …
Different Chemically Substituted Chitooligosaccharides Inhibit Beta-Secretase Activity, Rosna Binti Mat Taha
Different Chemically Substituted Chitooligosaccharides Inhibit Beta-Secretase Activity, Rosna Binti Mat Taha
Rosna Binti Mat Taha
Chitooligosaccharide or COS is a kind of oligosaccharide that is an integral part of the nervous system. COS is synthesized by chemical substitution and derived AE-COS, DEAE-COS and DMAE-COS that are being shown on IC(50) value. AE-COS and DMAE-COS exhibited four fold less inhibition than DEAE-COS. In addition, non-competitive inhibitor was identified via a Dixon plot and the Ki inhibition constant (100 mu g/mL). We declared, chemical substitution of COS is a water soluble human safe BACE-1 inhibitor.
Total Phenolic Contents, Antioxidant And Antimicrobial Activities Of Bruguiera Gymnorrhiza, Rosna Binti Mat Taha
Total Phenolic Contents, Antioxidant And Antimicrobial Activities Of Bruguiera Gymnorrhiza, Rosna Binti Mat Taha
Rosna Binti Mat Taha
In the present work, the antioxidant and antibacterial activities of methanolic, ethanolic and chloroform crude extract of leaves and barks of Bruguiera gymnorrhiza were investigated. The antioxidant activity of the crude extracts were evaluated using the enzymatic and non enzymatic methods namely superoxide dismutase determination, reducing power assay and 2, 2-diphenyl-1-picrylhydrazil free radical scavenging assays. Folin-ciocalteu reagent method was used to estimate the amount of total phenolic compounds of the extracts. Ethanol extract of barks showed the best result in all antioxidant assays which was positively co-related with the total phenolic contents. There was no significant difference between the IC(50) …
Effect Of Picloram, Additives And Plant Growth Regulators On Somatic Embryogenesis Of Phyla Nodiflora (L.) Greene, Rosna Binti Mat Taha
Effect Of Picloram, Additives And Plant Growth Regulators On Somatic Embryogenesis Of Phyla Nodiflora (L.) Greene, Rosna Binti Mat Taha
Rosna Binti Mat Taha
The present study describes the plant regeneration via somatic embryogenesis in suspension culture derived from the leaf and stem explants of Phyla nodiflora. The medium type, plant growth regulators, complex extract (coconut milk and malt extract) and anti-oxidant (activated charcoal, ascorbic acid, Polyvinylpyrrolidone and citric acid) markedly influenced the embryo regeneration of P. nodiflora. MS with 2,4-D and activated charcoal (10 mg/L) gave the highest stimulation of embryogenic callus growth. Optimized callus was transfered into suspension culture, which showed the globular, heart shaped embryos in MS with 2,4-D + BA + picloram (0.1 mg/L), coconut milk (10 ml/L), citric acid …
Antioxidant And Antibacterial Activities Of Ethanolic Extracts Of Asparagus Officinalis Cv. Mary Washington: Comparison Of In Vivo And In Vitro Grown Plant Bioactivities, Rosna Binti Mat Taha
Antioxidant And Antibacterial Activities Of Ethanolic Extracts Of Asparagus Officinalis Cv. Mary Washington: Comparison Of In Vivo And In Vitro Grown Plant Bioactivities, Rosna Binti Mat Taha
Rosna Binti Mat Taha
The antioxidant and antibacterial activities of ethanolic extracts of in vivo grown Asparagus officinalis cv. Mary Washington were investigated using superoxide dismutase, erythrocyte haemolysis and 2,2diphenyl-1-picrylhydrazil free radical scavenging methods. The measured antioxidant and antimicrobial potential were then compared to the activities offered by the ethanolic extracts of in vitro grown A. officinalis as well as ethanolic extract of undifferentiated callus cells of A. officinalis produced on Murashige and Skoog medium containing 1.5 mg/l 6-benzylaminopurine combined with 0.5 mg/l naphthalene acetic acid. The highest antioxidant capacity was obtained from the in vivo grown plant extract followed by in vitro grown …