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Full-Text Articles in Biotechnology
High-Throughput Imaging Of Neuronal Activity In Caenorhabditis Elegans, Dirk Albrecht, Johannes Larsch, Donovan Ventimiglia, Cornelia Bargmann
High-Throughput Imaging Of Neuronal Activity In Caenorhabditis Elegans, Dirk Albrecht, Johannes Larsch, Donovan Ventimiglia, Cornelia Bargmann
Dirk R. Albrecht
Neuronal responses to sensory inputs can vary based on genotype, development, experience, or stochastic factors. Existing neuronal recording techniques examine a single animal at a time, limiting understanding of the variability and range of potential responses. To scale up neuronal recordings, we here describe a system for simultaneous wide-field imaging of neuronal calcium activity from at least 20 Caenorhabditis elegans animals under precise microfluidic chemical stimulation. This increased experimental throughput was used to perform a systematic characterization of chemosensory neuron responses to multiple odors, odor concentrations, and temporal patterns, as well as responses to pharmacological manipulation. The system allowed recordings …
Multiphase Electropatterning Of Cells And Biomaterials, Dirk Albrecht, Gregory Underhill, Avital Mendelson§, Sangeeta Bhatia
Multiphase Electropatterning Of Cells And Biomaterials, Dirk Albrecht, Gregory Underhill, Avital Mendelson§, Sangeeta Bhatia
Dirk R. Albrecht
Tissues formed by cells encapsulated in hydrogels have uses in biotechnology, cell-based assays, and tissue engineering. We have previously presented a 3D micropatterning technique that rapidly localizes live cells within hydrogels using dielectrophoretic (DEP) forces, and have demonstrated the ability to modulate tissue function through the control of microscale cell architecture. A limitation of this method is the requirement that a single biomaterial must simultaneously harbor biological properties that support cell survival and function and material properties that permit efficient dielectrophoretic patterning. Here, we resolve this issue by forming multiphase tissues consisting of microscale tissue sub-units in a ‘local phase’ …
Photo- And Electropatterning Of Hydrogel-Encapsulated Living Cell Arrays, Dirk Albrecht, Valerie Tsang, Robert Sah, Sangeeta Bhatia
Photo- And Electropatterning Of Hydrogel-Encapsulated Living Cell Arrays, Dirk Albrecht, Valerie Tsang, Robert Sah, Sangeeta Bhatia
Dirk R. Albrecht
Living cells have the potential to serve as sensors, naturally integrating the response to stimuli to generate predictions about cell fate (e.g., differentiation, migration, proliferation, apoptosis). Miniaturized arrays of living cells further offer the capability to interrogate many cells in parallel and thereby enable high-throughput and/or combinatorial assays. However, the interface between living cells and synthetic chip platforms is a critical one wherein the cellular phenotype must be preserved to generate useful signals. While some cell types retain tissue-specific features on a flat (2-D) surface, it has become increasingly apparent that a 3-D physical environment will be required for others. …