Biodynamic Imaging And High-Content Analysis, Ran An, David D. Nolte
Dec 2013
Biodynamic Imaging And High-Content Analysis, Ran An, David D. Nolte
David D Nolte
The existence of phenotypic differences in the drug responses of 3D tissue relative to 2D cell culture is a concern in high-content drug screening. Biodynamic imaging is an emerging technology that probes 3D tissue using short-coherence dynamic light scattering to measure the intracellular motions inside tissues in their natural microenvironments. The information content of biodynamic imaging is displayed through tissue dynamics spectroscopy (TDS) but has not previously been correlated against morphological image analysis of 2D cell culture. In this article, a set of mitochondria-affecting compounds (FCCP, valinomycin, nicardipine, ionomycin) and Raf kinase inhibitors (PLX4032, PLX4720, GDC, and sorafenib) are applied …
Table Of Contents, David D. Nolte
Dec 2010
Table Of Contents, David D. Nolte
David D Nolte
Light is at once the most sensitive and the most gentle probe of matter. It is commonplace for light to measure a picometer displacement far below the nanometer scale of atoms, or to capture the emission of a single photon from a fluorescent dye molecule. Light is easy to generate using light-emitting diodes or lasers, and to detect using ultrasensitive photodetectors as well as the now ubiquitous digital cameras. Light also has the uncanny ability to penetrate living tissue harmlessly and deeply, while capturing valuable information on the health and function of cells. For these reasons, light has become an …
Review Of Biocd Physics And Technology, David D. Nolte
Dec 2008
Review Of Biocd Physics And Technology, David D. Nolte
David D Nolte
Spinning biodisks have advantages that make them attractive for specialized biochip applications. The two main classes of spinning biodisks are microfluidic disks and bio-optical compact disks BioCD. Microfluidic biodisks take advantage of noninertial pumping for lab-on-a-chip devices using noninertial valves and switches under centrifugal and Coriolis forces to distribute fluids about the disks. BioCDs use spinning-disk interferometry, under the condition of common-path phase quadrature, to perform interferometric label-free detection of molecular recognition and binding. The optical detection of bound molecules on a disk is facilitated by rapid spinning that enables high-speed repetitive sampling to eliminate 1/ f noise through common-mode …
