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Full-Text Articles in Biotechnology
Molecular Dynamics Simulations Provide Insight Into Stability Of Hyperthermophilic Endoglucanases, Logan E. Sheffield
Molecular Dynamics Simulations Provide Insight Into Stability Of Hyperthermophilic Endoglucanases, Logan E. Sheffield
Electronic Theses and Dissertations
Endoglucanases play a key role in the industrial production of bioethanol, but the most efficient method requires the utilization of high temperatures and is currently limited by the thermostability of endoglucanases. For this reason, it would be beneficial to discover more high-efficiency, thermostable enzymes to utilize in the hydrolytic process. In this study molecular dynamics simulations were performed on structurally similar endoglucanases with varying levels of thermostability to gain insight on what factors contribute to thermostability in endoglucanases. RMSD, RMSF, PCA, hydrogen bonding and salt bridges were analyzed. Finally, protein energy networks were constructed from nonbonded interaction potentials and analysis …
The Comparison Of Effects Of Synthetic And Natural Arachidin-3 On Rotavirus Infected Cells, Rebekah Napier-Jameson
The Comparison Of Effects Of Synthetic And Natural Arachidin-3 On Rotavirus Infected Cells, Rebekah Napier-Jameson
Electronic Theses and Dissertations
Rotavirus (RV) causes severe, life-threatening diarrhea, in infants, young children and immunocompromised adults. There are several effective vaccines for young children, however they are strain specific and are not protective against many RV strains in developing countries. Therefore, it is important to investigate anti-RV therapeutic agents. Our laboratory has shown arachidin-1 (A1) and arachadin-3 (A3) significantly inhibit RV replication in two cell lines, however the molecular mechanism(s) of action are not known. A synthetic molecule of A3 (sA3) has been produced, but its’ antiviral effects have not been examined. Our hypothesis is that sA3 produces the same effects on RV-infected …
The Effect Of Compound L19 On Human Colorectal Cells (Dld-1), Sepideh Mohammadhosseinpour
The Effect Of Compound L19 On Human Colorectal Cells (Dld-1), Sepideh Mohammadhosseinpour
Electronic Theses and Dissertations
Colorectal cancer is the third most common type of cancer in the world and the second leading cause of death among humans. Extracts of water soluble compounds from the roots and leaves of Rumex crispus were screened for compounds that induced apoptosis in DLD-1 cells. A compound referred to as L19 was isolated using Accelerated Solvent Extraction (ASE) followed by High Performance Liquid Chromatography (HPLC). Gas chromatography coupled with mass spectrometry was used to identify the L19 as a tetrahydrofuran with a molecular weight of 72.11 g/mole. Each HPLC fraction resulted in 94.4% purity. In the present research, specific genes …
Cloning, Purification, And Biochemical Characterization Of Human Prolyl Endopeptidase, Travis K. Moore
Cloning, Purification, And Biochemical Characterization Of Human Prolyl Endopeptidase, Travis K. Moore
Electronic Theses and Dissertations
Eurygaster integriceps Puton, common name sunn Pest, is one of the primary sources of wheat crop wastes in North Africa, Middle East, and Eastern Europe. It feeds by injecting the wheat grain with an enzyme characterized as prolyl endoprotease (spPEP) that breaks down Gluten, the wheat’s main constitutive protein necessary for bread production (Darkoh et al., 2010). Previously, it has been shown that peptides isolated from Lactobacillus hydrolysates of caseins in bovine milk are able to inhibit mammalian PEP in colon cells, as well as bacterial PEP (Juillerat-Jeanneret et al., 2010). While recombinant versions of these peptides are also potential …