Biotechnology Commons^™

The Use Of Potato (Solanum Tuberosum) Suspension Cells For Rapid Screening Of Chloroplast Transformation Vectors, Lindsey Shover

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Chloroplast genetic engineering is unique in that it allows for high levels of gene expression while providing a means of natural bio-confinement. Although the chloroplast genome sequence of over 800 plant species is known, the elements that naturally regulate chloroplast gene expression are poorly understood. Recently, our lab has developed a 264 part modular cloning kit that contains known chloroplast regulatory elements. This kit can be used for the construction of novel chloroplast transformation cassettes; however, functional testing of these cassettes is currently hindered by standard chloroplast transformation technologies. Therefore, the aim of this experiment was to develop a rapid …