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Full-Text Articles in Biotechnology

Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh Nov 2000

Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh

Faculty Works

The hybridization of DNA by sequential electrostatic and hydrogen-bonding immobilization of single-stranded complementary oligonucleotides at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during each stage of complexation in the following sequence: primary single-stranded DNA followed by complementary single-stranded DNA followed by the intercalator, ethidium bromide. Langmuir-Blodgett (LB) films of the ODA-DNA complex were formed on different substrates and characterized using quartz-crystal microgravimetry (QCM), Fourier transform infrared …


The Risks And Benefits Of Genetically Modified Crops: A Multidisciplinary Perspective, Garry D. Peterson, Saul Cunningham, Lisa Deutsch, Jon Erickson, Allyson Quinlan, Ernesto Ráez-Luna, Robert Tinch, Max Troell, Peter Woodbury, Scot Zens Mar 2000

The Risks And Benefits Of Genetically Modified Crops: A Multidisciplinary Perspective, Garry D. Peterson, Saul Cunningham, Lisa Deutsch, Jon Erickson, Allyson Quinlan, Ernesto Ráez-Luna, Robert Tinch, Max Troell, Peter Woodbury, Scot Zens

Dartmouth Scholarship

Worldwide, the area planted in genetically modified (GM) crops has increased dramatically in recent years. Between 1996 and 1999, it rose from 1.6 X 106 ha to more than 35 X 106 ha (James 1998, May 1999). This rapid increase has provoked an explosion of concern, particularly in Europe, over the health and environmental impacts of these crops. Despite claims of safety and warnings against popular panic, public concern over GM crops has resulted in changes in their marketing, labeling, planting, and trade. These changes have fueled an increasingly heated debate among environmental advocates, critics of industrial agriculture, …