Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Biotechnology
Mcnamara 201412 Nih Scap Innocentive Challenge Solution - T-Bow Rainbow T-Cells And Tumor Cells Spatial Multiplexing Gene Expression Reporter System – Plus Supplement Plus Posters - 20151027 - Please Download "75" Instead, George Mcnamara
George McNamara
McNamara 201412 NIH SCAP InnoCentive Challenge Solution - T-Bow Rainbow T-cells and Tumor Cells Spatial Multiplexing Gene Expression Reporter System – plus supplement plus posters - 20151027.
///
Please download the current 20151027 (October 27, 2015) Tattletales and T-Bow update from
http://works.bepress.com/gmcnamara/75/
The bepress web site is not letting me replace the old pdf here at "65" with the additional 10 pages update.
///
The download is my/Cooper lab solution (submission) to the 2014 NIH Single Cell Analysis Program (SCAP) InnoCentive Challenge, "Follow That Cell". I submitted the Solution on 20141215Mon (with 20 minutes to spare). The Challenge web page …
Tattletales And T-Bow Update 20151027tue, George Mcnamara
Tattletales And T-Bow Update 20151027tue, George Mcnamara
George McNamara
20151027Tue this "75"
http://works.bepress.com/gmcnamara/75
is my update of "65" posting
See text at
http://works.bepress.com/gmcnamara/65/
for text summary. The PDf here in "75" supersedes "65".
The PDF here has 10 pages added to the end from the "65" version (pages 40-49 of PDF when including the bepress cover page)..
here is the text in my cover page (bepress may add its own cover):
20151027Tue: added 10 page e-poster at bottom explaining Binary Tattletales and T-Bow. That is, binary with respect to protein components. For one color (number of repeats, epitope tags, FPs are examples, here rounded to convenient numbers):
1. 100 …
Tattletales And T-Bow Update 20140602mon, George Mcnamara
Tattletales And T-Bow Update 20140602mon, George Mcnamara
George McNamara
Tattletales and T-Bow Update 20140602Mon
http://works.bepress.com/gmcnamara/42
Please see also http://works.bepress.com/gmcnamara/26
Tattletales: multiplex fluorescent protein biosensors by spatial localization with TALE-FPs, Cas9-FPs, ZF-FPs, LacI-FPs, TetR-FPs, etc.
T-Bow: Rainbow T-cells and Tumor cells (and ES cells, iPS cells, other cells and organisms). You can think of this as "Brainbow meets TALENs/Cas9/ZFNs/other DNA sequence specific binding proteins".
If not familiar with Brainbow, see
http://en.wikipedia.org/wiki/Brainbow
If not familiar with TALENs, Cas9, etc, see
http://www.addgene.org/genome_engineering/
Big idea: localizing fluorescent proteins - and/or Nano-Lanterns (Take Nagai) - to tandem repeat arrays - is a great way to improve signal to noise ratio compared to the usual …