Open Access. Powered by Scholars. Published by Universities.®

Biotechnology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 21 of 21

Full-Text Articles in Biotechnology

Distinct Cardiac Responses To Heat Stress Between Two Broiler Lines Identified By Transcriptome Analysis, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont Jan 2016

Distinct Cardiac Responses To Heat Stress Between Two Broiler Lines Identified By Transcriptome Analysis, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont

Jibin Zhang

Prolonged exposure to high temperature can significantly increase metabolism, depress appetite, reduce meat production and raise mortality in broilers. Because the cardiovascular system plays an important role to dissipate heat and transport oxygen, strong cardiac function is indispensable for birds to survive under heat stress. However, due to small hearts relative to body weight, modern broilers have high risk of heart failure at high temperature.
In this study, a Ross 708 line (modern broilers) and a Heritage broiler line (relaxed selection since the 1950s) were subjected to heat stress at 35℃  for 7 hours/day from 21 to 42 days posthatch …


Differential Expression Of Cell Cycle Regulators During Hyperplastic And Hypertrophic Growth Of Broiler Subcutaneous Adipose Tissue.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Paula Chen, Michael Davis, Kichoon Lee May 2015

Differential Expression Of Cell Cycle Regulators During Hyperplastic And Hypertrophic Growth Of Broiler Subcutaneous Adipose Tissue.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Paula Chen, Michael Davis, Kichoon Lee

Jibin Zhang

Hyperplasticgrowth and hypertrophicgrowth within adiposetissue is tightly associated with cellcycle activity. In this study, CCNG2 and CDKN2C were found to be correlated with cellcycle inhibition during fat cell differentiation, whereas CCND3, CCNA1, and ANAPC5 were positively associated with cellcycle activity during fat cell proliferation after selection based on GEO datasets available on the NCBI website. The findings were validated through comparison of expressions of these genes among different tissues/fractions in broiler chickens and time points during primary cell culture using quantitative real-time PCR. Development of broilersubcutaneousadiposetissue was investigated on embryonic days 15 and 17 and on post-hatch days 0, 5, …


Prevalence And Effects Of Mycotoxins On Poultry Health And Performance, And Recent Development In Mycotoxin Counteracting Strategies, G. R. Murugesan, D Ledoux, K Naehrer, F Berthiller, T Applegate, B Grenier, T Phillips, G Schatzmayr Dec 2014

Prevalence And Effects Of Mycotoxins On Poultry Health And Performance, And Recent Development In Mycotoxin Counteracting Strategies, G. R. Murugesan, D Ledoux, K Naehrer, F Berthiller, T Applegate, B Grenier, T Phillips, G Schatzmayr

G. R. Murugesan

Extensive research over the last couple of decades has made it obvious that mycotoxins are commonly prevalent in majority of feed ingredients. A worldwide mycotoxin survey in 2013 revealed 81 % of around 3,000 grain and feed samples analyzed had at least one mycotoxin which was higher than the ten-year average (from 2004 to 2013) of 76% in a total of 25,944 samples. The considerable increase in the number of positive samples in 2013 may be due to the improvements in detection methods and their sensitivity. The recently developed liquid chromatography coupled to (tandem) mass spectrometry allows the inclusion of …


Trait Selection And Welfare Of Genetically Engineered Animals In Agriculture, Michael Greger May 2014

Trait Selection And Welfare Of Genetically Engineered Animals In Agriculture, Michael Greger

Michael Greger, MD, FACLM

The release of the Final Guidance from the US Food and Drug Administration on the commercialization of genetically engineered animals has sparked renewed discussion over the ethical, consumer, and regulatory implications of transgenesis in animal agriculture. Animal welfare critiques have focused on unexpected phenotypic effects in animals used in transgenic research, rather than on the health and welfare implications of the intended productivity enhancement. Unless breeding goals are redefined to reflect social concerns, the occurrence and magnitude of undesirable side effects may increase and consumer confidence in the nascent technology may be undermined.


Differential Expression Of Cyclin G2, Cyclin Dependent Kinase Inhibitor 2c And Peripheral Myelin Protein 22 Genes During Adipogenesis..Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Jinsoo Ahn, Michael E. Davis, Kichoon Lee May 2014

Differential Expression Of Cyclin G2, Cyclin Dependent Kinase Inhibitor 2c And Peripheral Myelin Protein 22 Genes During Adipogenesis..Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Jinsoo Ahn, Michael E. Davis, Kichoon Lee

Jibin Zhang

Increase of fat cells (FCs) in adipose tissue is attributed to proliferation of preadipocytes or immature adipocytes in the early stage, as well as adipogenic differentiation in the later stage of adipose development. Although both events are involved in the FC increase, they are contrary to each other, because the former requires cell cycle activity, whereas the latter requires cell cycle withdrawal. Therefore, appropriate regulation of cell cycle inhibition is critical to adipogenesis. In order to explore the important cell cycle inhibitors and study their expression in adipogenesis, we adopted a strategy combining the Gene Expression Omnibus (GEO) database available …


A Comprehensive Microrna Expression Profile Of The Backfat Tissue From Castrated And Intact Full-Sib Pair Male Pigs.Pdf, Ying Bai, Jinming Huang, Gang Liu, Jibin Zhang, Jianying Wang, Chengkun Liu, Meiying Fang Jan 2014

A Comprehensive Microrna Expression Profile Of The Backfat Tissue From Castrated And Intact Full-Sib Pair Male Pigs.Pdf, Ying Bai, Jinming Huang, Gang Liu, Jibin Zhang, Jianying Wang, Chengkun Liu, Meiying Fang

Jibin Zhang

Background: It is widely known that castration has a significant effect on the accumulation of adipose tissue. microRNAs (miRNAs) are known to be involved in fat deposition and to be regulated by the androgen-induced androgen receptor (AR). However, there is little understanding of the relationship between miRNAs and fat deposition after castration. In this study, the high-throughput SOLiD sequencing approach was used to identify and characterize miRNA expression in backfat from intact and castrated full-sib male 23-week-old pigs. The patterns of adipogenesis and fat deposition were compared between castrated and intact male pigs.
Results: A total of 366 unique miRNA …


Acute Toxicity Of Copper Sulfate And Potassium Dichromate On Stygobiont Proasellus: General Aspects Of Groundwater Ecotoxicology And Future Perspectives, Ana Reboleira, Nelson Abrantes, Pedro Oromí, Fernando Gonçalves Mar 2013

Acute Toxicity Of Copper Sulfate And Potassium Dichromate On Stygobiont Proasellus: General Aspects Of Groundwater Ecotoxicology And Future Perspectives, Ana Reboleira, Nelson Abrantes, Pedro Oromí, Fernando Gonçalves

Ana Sofia P.S. Reboleira

Karst systems harbor large groundwater resources for human consumption and represent an important habitat for rare and unprotected specialized animals, the so-called stygofauna. Due to the highly adapted features towards underground life, together with the geographic isolation provided by the subterranean aquifers, groundwater-dwelling animals may lose the ability to face sudden changes on their ecosystems, and therefore the risk of extinction is remarkably high. A little is known about their sensitiveness, especially linked to contamination pressure in urbanized karst areas. Understanding the impact of contaminants on stygofauna is important for setting groundwater environmental quality and management of karst systems. We …


Fractal Approach To Heat Transfer In Silkworm Cocoon Hierarchy, Dong-Dong Fei, Fu-Juan Liu, Qiu-Na Cui, Ji-Huan He Jan 2013

Fractal Approach To Heat Transfer In Silkworm Cocoon Hierarchy, Dong-Dong Fei, Fu-Juan Liu, Qiu-Na Cui, Ji-Huan He

Ji-Huan He

Silkworm cocoon has a complex hierarchic structure with discontinuity. In this paper, heat transfer through the silkworm cocoon is studied using fractal theory. The fractal approach has been successfully applied to explain the fascinating phenomenon of cocoon survival under extreme temperature environment. A better understanding of heat transfer mechanisms for the cocoon could be beneficial to the design of biomimetic clothes for special applications.


Viability Of Lactic Acid Bacteria And Sensory Evaluation In Cinnamomum Verum And Allium Sativum-Bio-Yogurts Made From Camel And Cow Milk, Ahmad Salihin Hj Baba, Shori A. B. Dec 2011

Viability Of Lactic Acid Bacteria And Sensory Evaluation In Cinnamomum Verum And Allium Sativum-Bio-Yogurts Made From Camel And Cow Milk, Ahmad Salihin Hj Baba, Shori A. B.

Ahmad Salihin Hj Baba

The present study investigate the effect of herbal water extract prepared from Allium sativum and Cinnamomum verum on the viability of lactic acid bacteria (Lactobacillus spp and Streptococcus thermophilus) in cow- and camel-milk yogurts during 21 day refrigerated storage. The organoleptic properties of fresh-yogurts were evaluated. Lactobacillus spp count for fresh cow milk-yogurts (0 day) in both present and absent of C. verum and A. sativum was ranged from 1.4×0 6 to 2.1×10 6 cfu/mL. These values were not significantly changed throughout the 21 days of refrigerated storage. Lactobacillusspp count in fresh plain camel milk- yogurt was 13.2×10 6 cfu/mL …


Expression Analysis Of Hif-1a And Hif-2a Genes In Tibetan Chicken Under Normoxia And Hypoxia.Pdf, Yunzhou Yang, Ying Bai, Xinxing Dong, Jibin Zhang, Meiying Fang Nov 2011

Expression Analysis Of Hif-1a And Hif-2a Genes In Tibetan Chicken Under Normoxia And Hypoxia.Pdf, Yunzhou Yang, Ying Bai, Xinxing Dong, Jibin Zhang, Meiying Fang

Jibin Zhang

Tibetan chicken is one of those chicken breeds that could adapt to high altitude and low oxygen pressure environment; it owes to an integrative genetic mechanism for hypoxia adaptability compared to lowland chicken breeds. HIF-1α and HIF-2α are the central factors playing important roles in maintaining organisms’s oxygen homeostasis. In this study, the expression of HIF-1α and HIF-2α genes were investigated by Real Time-PCR in Tibetan chicken and Dwarf chicken embryo brain tissue under normoxia (21% oxygen concentration) and hypoxia (13% oxygen concentration). The results showed that Tibetan chicken always had lower mortality than Dwarf chicken during the incubation. We …


Effect Of Starch Sources And Protein Content On Extruded Aquaculture Feed Containing Ddgs, Sankaranandh Kannadhason, Kasiviswanathan Muthukumarappan, Kurt A. Rosentrater Feb 2011

Effect Of Starch Sources And Protein Content On Extruded Aquaculture Feed Containing Ddgs, Sankaranandh Kannadhason, Kasiviswanathan Muthukumarappan, Kurt A. Rosentrater

Kurt A. Rosentrater

A 3 × 3 × 3 completely randomized design was used to investigate extrusion cooking behavior and product characteristics of distillers dried grains with solubles (DDGS), protein levels, and various starch sources in a laboratory scale single screw extruder. Cassava, corn, and potato starches with varying levels of DDGS (20%, 30%, and 40% wet basis (wb)) were extruded with three different proportions of protein levels (28%, 30%, and 32% wb). The extrusion cooking was performed at a constant feed moisture content of 20% wb, barrel temperature of 120 °C, and a preset screw speed of 130 rpm (13.6 rad/s). Extrudate …


Socioeconomic Characteristics And Perceptions Of Cattle Keepers And Constraints To Cattle Production In Western Kenya, Joshua O. Amimo, S Thumbi, B O. Inyangala, J O. Junga, R O. Mosi Jan 2011

Socioeconomic Characteristics And Perceptions Of Cattle Keepers And Constraints To Cattle Production In Western Kenya, Joshua O. Amimo, S Thumbi, B O. Inyangala, J O. Junga, R O. Mosi

Joshua O Amimo

No abstract provided.


Factors Influencing Reproductive Performance Of Cows From Different Nguni Ecotypes In Southern Mozambique, S Maciel, O Mwai, Joshua O. Amimo, S Moyo, M Scholtz, F Neser, M Martins Jan 2011

Factors Influencing Reproductive Performance Of Cows From Different Nguni Ecotypes In Southern Mozambique, S Maciel, O Mwai, Joshua O. Amimo, S Moyo, M Scholtz, F Neser, M Martins

Joshua O Amimo

No abstract provided.


Molecular Cloning And Characterization Of The Porcine Fto Promoter And Coding Regions.Pdf, Jinming Huang, Yunzhou Yang, Gang Liu, Jibin Zhang, Xinxing Dong, Ying Bai, Meiying Fang Nov 2010

Molecular Cloning And Characterization Of The Porcine Fto Promoter And Coding Regions.Pdf, Jinming Huang, Yunzhou Yang, Gang Liu, Jibin Zhang, Xinxing Dong, Ying Bai, Meiying Fang

Jibin Zhang

The fat mass and obesity associated gene (FTO) has been widely reported to be associated with fat mass or fat deposition in different species. In the present study, we cloned both promoter and codingregions of the gene in pigs with over 5 Kb of sequence for the former region and 1,596 bp for the latter region. Comparative analysis of the promoter region among 20 species including pig revealed four conserved regions that harbor transcriptional factors involved in adipose differentiation. Using a pooled DNA sequencing approach, we discovered 39 single nucleotide polymorphisms (SNPs) in the pig FTO gene and four of them were …


Effects Of Processing Conditions On Single Screw Extrusion Of Feed Ingredients Containing Ddgs, Nehru Chevanan, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan Feb 2010

Effects Of Processing Conditions On Single Screw Extrusion Of Feed Ingredients Containing Ddgs, Nehru Chevanan, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan

Kurt A. Rosentrater

Distillers dried grains with solubles (DDGS), a feed coproduct from the fuel ethanol industry, has been shown to be a viable potential alternative protein source for aquaculture feeds. To investigate this, three isocaloric (3.5kcal/g) ingredient blends containing 20, 30, and 40% DDGS, with a net protein adjusted to 28% (wet basis, wb), were prepared for use as Nile tilapia feed. Extrusion processing was then conducted using three DDGS contents (20, 30, and 40%, wb), three moisture contents (15, 20, and 25%, wb), three barrel temperature gradients (90–100–100°C, 90–130–130°C, and 90–160–160°C), and five screw speeds (80, 100, 120, 140, and 160rpm) …


Polyphyly Of The Pikeminnows (Teleostei: Cyprinidae) Inferred Using Mitochondrial Dna Sequences, T. Heath Ogden Dec 2009

Polyphyly Of The Pikeminnows (Teleostei: Cyprinidae) Inferred Using Mitochondrial Dna Sequences, T. Heath Ogden

T. Heath Ogden

The phylogenetic relationships of the Colorado pikeminnow Ptychocheilus lucius, northern pikeminnow P. oregonensis, Sacramento pikeminnow P. grandis, Umpqua pikeminnow P. umpquae, and hardhead Mylopharodon conocephalus were examined by using molecular data to investigate monophyly of the genus Ptychocheilus. Phylogenies generated using DNA sequence data from the cytochrome b and 16S ribosomal DNA genes of the mitochondrial genome reveal that Ptychocheilus is a polyphyletic genus and suggest that the taxonomy of the group is in need of further revision. These data yield insights into the evolution of the pikeminnows and help place the significant evolutionary events in context with the geological …


Extrusion Studies Of Aquaculture Feed Using Distillers Dried Grains With Solubles And Whey, Nehru Chevanan, Kasiviswanathan Muthukumarappan, Kurt A. Rosentrater Jun 2009

Extrusion Studies Of Aquaculture Feed Using Distillers Dried Grains With Solubles And Whey, Nehru Chevanan, Kasiviswanathan Muthukumarappan, Kurt A. Rosentrater

Kurt A. Rosentrater

Three isocaloric (3.5 kcal/g) ingredient blends containing 20, 30, and 40% distiller-dried grains with solubles (DDGS) along with 5% whey were prepared with a net protein content adjusted to 28% (wet basis [wb]). Other ingredients in the blends included soy flour, corn flour, fish meal, vitamin, and mineral mix. These blends were extruded in a single-screw extruder at 15, 20, and 25% (wb) moisture content and at 130 and 160 rpm screw speeds. Compared to previous research, the durability and unit density of the extrudates in this study were found to increase substantially by the addition of whey to the …


The Effects Of Non-Genetic Factors And Estimation Of Genetic And Phenotypic Parameters And Trends For Milk Yield In Ayrshire Cattle In Kenya, Joshua O. Amimo, J W. Wakhungu, B O. Inyangala, R O. Mosi Jan 2007

The Effects Of Non-Genetic Factors And Estimation Of Genetic And Phenotypic Parameters And Trends For Milk Yield In Ayrshire Cattle In Kenya, Joshua O. Amimo, J W. Wakhungu, B O. Inyangala, R O. Mosi

Joshua O Amimo

No abstract provided.


An Assessment Of The Efficiency Of The Dairy Bull Dam Selection Methodology In Kenya, J Ombura, J W. Wakhungu, R O. Mosi, Joshua O. Amimo Jan 2007

An Assessment Of The Efficiency Of The Dairy Bull Dam Selection Methodology In Kenya, J Ombura, J W. Wakhungu, R O. Mosi, Joshua O. Amimo

Joshua O Amimo

No abstract provided.


Phenotypic And Genetic Parameters Of Reproductive Traits For Ayrshire Cattle On Large-Scale Farms In Kenya, Joshua O. Amimo Jan 2006

Phenotypic And Genetic Parameters Of Reproductive Traits For Ayrshire Cattle On Large-Scale Farms In Kenya, Joshua O. Amimo

Joshua O Amimo

No abstract provided.


Growth And Reproductive Performance Of Small Ruminants Under Integrated Livestock Oil Palm Production System, Ahmad Salihin Hj Baba, Azillah A., Mukherjee T. K., Abdullah R. B. Dec 1997

Growth And Reproductive Performance Of Small Ruminants Under Integrated Livestock Oil Palm Production System, Ahmad Salihin Hj Baba, Azillah A., Mukherjee T. K., Abdullah R. B.

Ahmad Salihin Hj Baba

The effects of supplementation with mixed fodder or concentrate were investigated on the performance of a) growth of male lambs;md kids grazing extensively under oil palm trees and b) reproduction of ewes and goats stall-fed with native herbage. Supplemented kids and lambs had higher average daily weight gain than controls but the effect was only significant for those which received concentrate (p < 0.05). Final body weight was only significantly different from controls for lambs supplemented with concentrate (p < 0.05). The ages at puberty, first mating, first conception and first kidding of supplemented goats were about 110 days earlier than those for controls (p < 0.05 or better). Supplemented goats had first mating and conceived at lower body weights (p < 0.01) than those in control groups. Different feeding regimes had no effects (p > 0.05) on the reproductive performance of ewes apart fi om highest body weight of first lambing in animals supplemented with concentrate (p < 0.05). Native herbage available under oil palm trees of 5 years old was sufficient for growth …