Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Biotechnology
Regression Analysis On Ionic Liquid Pretreatment Of Sugarcane Bagasse And Assessment Of Structural Changes, Li Wan Yoon, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
Regression Analysis On Ionic Liquid Pretreatment Of Sugarcane Bagasse And Assessment Of Structural Changes, Li Wan Yoon, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
Teck Nam Ang Dr.
This study aims to perform a regression analysis which leads to the optimization on the operating conditions of ionic liquid (IL), 1-ethyl-3-methylimidazolium acetate ([EMIM]oAc) pretreatment on sugarcane bagasse (SCB). The structural changes on SCB during pretreatment were also examined. The effects of temperature, time and solid loading on reducing sugar (RS) yield obtained from enzymatic hydrolysis of pretreated SCB were investigated by applying Central Composite Design (CCD) of Response Surface Methodology (RSM). Results from CCD were modeled into a second order polynomial equation and the model shows a good correlation between predicted and experimental values. The optimized condition for [EMIM]oAc …
A Quantitative Method For Fungal Ligninolytic Enzyme, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
A Quantitative Method For Fungal Ligninolytic Enzyme, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
Teck Nam Ang Dr.
Screening is conducted to select a fungus with desired characteristics intended for various applications, e.g. bioremediation and enzyme production. A qualitative method was used in this study for screening of fungal ligninolytic enzymes activities. The fungal ligninolytic activity was correlated with its growth from the screenings to identify a suitable fungus for solid substrate fermentation. Four strains of fungi, namely Phanerochaete chrysosporium, Pycnoporus sanguineus, Phlebia radiata and Pleurotus sajor-caju, were screened for their ligninolytic enzymes activities using guaiacol and Remazol Brilliant Blue-R (RBB-R) as screening reagents. The screenings were conducted at both the optimal growth temperature of each fungus and …