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Method For The Detection Of 17-B-Estradiol In Wastewater Facility Effluents Using Hplc, Alexa Mariah Fisher, Benjamin J. Thornton
Method For The Detection Of 17-B-Estradiol In Wastewater Facility Effluents Using Hplc, Alexa Mariah Fisher, Benjamin J. Thornton
Research in Biology
Studies of the effects of estrogen in aquatic ecosystems largely focus on fish. In fish, estrogen reduces fecundity in females, reduces testicular development and fertility in males, and alters vitellogenin production in both sexes. One way estrogens enter aquatic environments is via wastewater effluents. Effluent samples from the Moccasin Bend Wastewater Treatment Facility in Chattanooga, Tennessee were tested for the presence of 17-beta-estradiol (E2), using an Agilent 1260 Infinity LC (HPLC). We were not able to detect the presence of E2 in these samples with the methods described. However, when effluent samples were spiked with stock E2 (final concentration of …
Hplc Detection Of The Possible Presence Of 17-A-Ethindyl Estradiol In Treated Effluents Released From The Chattanooga Water Treatment Plant, Jacqueline Dulanto, Benjamin Thornton, David Nelsen
Hplc Detection Of The Possible Presence Of 17-A-Ethindyl Estradiol In Treated Effluents Released From The Chattanooga Water Treatment Plant, Jacqueline Dulanto, Benjamin Thornton, David Nelsen
Research in Biology
Research has confirmed that elevated synthetic estrogen in surface waters can lead to intersex characteristics in aquatic vertebrates. Unmetabolized antibiotics, hormones from animal wastes, including humans, and discarded pharmaceuticals are some ways synthetic estrogen enter aquatic ecosystems through the release of contaminated effluents. In this investigation, the Agilent 1260 Infinity HPLC was used to detect the possible presence of 17-α-ethindyl estradiol in effluents released from the Chattanooga Sewer Treatment Plant. Results were analyzed by comparing HPLC chromatograms from effluent and spiked samples. We detected a possible peak of synthetic estrogen in the effluent samples with a retention rate of 6 …