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1996

Selected Works

Articles 1 - 13 of 13

Full-Text Articles in Biology

The Rna-Binding Site Of Bacteriophage Qβ Coat Protein, Francis Lim, Marc Spingola, David S. Peabody Dec 1996

The Rna-Binding Site Of Bacteriophage Qβ Coat Protein, Francis Lim, Marc Spingola, David S. Peabody

Marc Spingola

The coat proteins of the RNA bacteriophages Qβ and MS2 are specific RNA binding proteins. Although they possess common tertiary structures, they bind different RNA stem loops and thus provide useful models of specific protein-RNA recognition. Although the RNA-binding site of MS2 coat protein has been extensively characterized previously, little is known about Qβ. Here we describe the isolation of mutants that define the RNA-binding site of Qβ coat protein, showing that, as with MS2, it resides on the surface of a large β-sheet. Mutations are also described that convert Qβ coat protein to the RNA binding specificity of MS2. …

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Improved Cultivation Systems For Isolation Of The Colorado Potato Beetle Spiroplasma., Meghnad Konai, K. J. Hackett, D. L. Williamson, J. J. Lipa, J. D. Pollack, G. E. Gasparich, E. A. Clark, D. C. Vacek, R. F. Whitcomb Aug 1996

Improved Cultivation Systems For Isolation Of The Colorado Potato Beetle Spiroplasma., Meghnad Konai, K. J. Hackett, D. L. Williamson, J. J. Lipa, J. D. Pollack, G. E. Gasparich, E. A. Clark, D. C. Vacek, R. F. Whitcomb

Gail Gasparich

In North America, the Colorado potato beetle, Leptinotarsa decemlineata, is often infected with the host-specific, gut-inhabiting Colorado potato beetle spiroplasma (CPBS). CPBS is apparently a commensal, but it may be useful in biocontrol if it can be transformed to express an insect-lethal gene. Difficulty in cultivating the organism, however, has hindered the development of a suitable transformation system. In this study, we eliminated the need for coculturing CPBS with insect cells. CPBS was reliably isolated with the BBL Anaerobic GasPak Jar system (low redox, enhanced CO(inf2)), which was easier to use and less expensive than insect cell coculture methods. A …

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Developmental And Tissue-Specific Expression Of Mouse Pelle-Like Protein Kinase, Marina Trofimova, Amy B. Sprenkle, Melissa Green, Thomas W. Sturgill, Mark G. Goebl, Maureen A. Harrington Jul 1996

Developmental And Tissue-Specific Expression Of Mouse Pelle-Like Protein Kinase, Marina Trofimova, Amy B. Sprenkle, Melissa Green, Thomas W. Sturgill, Mark G. Goebl, Maureen A. Harrington

Amy Sprenkle

The NF-kappaB/c-Rel proteins are a family of evolutionarily conserved transcription factors activated during development that in the adult, mediate many processes including the immune response. A high degree of sequence similarity is shared between the NF-kappaB/c-Rel family of transcription factors and the Drosophila Dorsal protein as well as between its cytoplasmic inhibitor, IkappaBalpha, and the Drosophila Cactus protein. Genetic analyses of Dorsal have defined components of a signaling pathway for Dorsal activation, including a serine/threonine kinase, Pelle, placed upstream of Dorsal and Cactus. We demonstrate that this pathway is likely to be conserved in mammals by the isolation of a …

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Pseudomonas Aeruginosa And Epithelial Permeability: Role Of Virulence Factors Elastase And Exotoxin A., Ali Azghani Jun 1996

Pseudomonas Aeruginosa And Epithelial Permeability: Role Of Virulence Factors Elastase And Exotoxin A., Ali Azghani

Ali Azghani

Lung injury in bacterial infection is a multifactorial phenomenon that involves bacterial metabolites and host factors. Primary isolates of type II pneumocytes and established cultures of Madin-Darby canine kidney (MDCK) cells were used to study effects of Pseudomonas aeruginosa exoproducts on epithelial paracellular permeability. The results indicate that elastase (PE) and exotoxin A (Exo A) have different, but complementary, actions that diminish epithelial barrier function. We measured transepithelial electrical resistance (TER) and permeability coefficient for mannitol (Pm) across cell monolayers plated on tissue culture membranes. Application of 100 ng/ml of Exo A to the basal side decreased TER from 1,405 …

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Using Www Databases In Investigative Laboratory Projects For Plant Physiology And Molecular Biology, Jonathan Monroe, I. Knight Dec 1995

Using Www Databases In Investigative Laboratory Projects For Plant Physiology And Molecular Biology, Jonathan Monroe, I. Knight

Jonathan D. Monroe

No abstract provided.

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Effects Of A Low-Intensity, Winter Burn On Long-Unburned Sand Pine Scrub, W. Abrahamson, J. Abrahamson Dec 1995

Effects Of A Low-Intensity, Winter Burn On Long-Unburned Sand Pine Scrub, W. Abrahamson, J. Abrahamson

Warren G. Abrahamson, II

No abstract provided.

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The Avian Communities Of A Suburban Grassland Refugium: Population Studies At An Airport In Northeastern United States, D Caccamise, L Reed, L Delay, K Bennett, Jerald Dosch Dec 1995

The Avian Communities Of A Suburban Grassland Refugium: Population Studies At An Airport In Northeastern United States, D Caccamise, L Reed, L Delay, K Bennett, Jerald Dosch

Jerald Dosch, PhD

No abstract provided.

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Features Of Roots Of Cam Plants, P. Nobel, Gretchen North Dec 1995

Features Of Roots Of Cam Plants, P. Nobel, Gretchen North

Gretchen North

No abstract provided.

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Montandon Marsh: A Vegetation Description Of A Potentially Endangered Wetland, E. Hochman, W. Abrahamson, J. Clark Dec 1995

Montandon Marsh: A Vegetation Description Of A Potentially Endangered Wetland, E. Hochman, W. Abrahamson, J. Clark

Warren G. Abrahamson, II

No abstract provided.

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Radial Hydraulic Conductivity Of Individual Root Tissues Of Opuntia Ficus-Indica (L.) Miller As Soil Moisture Varies, Gretchen North, P. Nobel Dec 1995

Radial Hydraulic Conductivity Of Individual Root Tissues Of Opuntia Ficus-Indica (L.) Miller As Soil Moisture Varies, Gretchen North, P. Nobel

Gretchen North

The constraints on water uptake imposed by individual root tissues were examined for Opuntia ®cus-indica under wet, drying, and rewetted soil conditions. Root hydraulic conductivity (L P ) and axial conductance (K h ) were measured for intact root segments from the distal region with an endodermis and from midroot with a periderm; L P was then measured for each segment with successive tissues removed by dissection. Radial conductivity (L R ) was calculated from L P and K h for the intact segment and for the individual tissues by considering the tissue conductivities in series. Under wet conditions, L …

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Bayk 8644 Stimulates Calcium Release From Skeletal Muscle Sr, Chris Palahniuk, D. Bose, R. Bose, J. Gilchrist Dec 1995

Bayk 8644 Stimulates Calcium Release From Skeletal Muscle Sr, Chris Palahniuk, D. Bose, R. Bose, J. Gilchrist

Chris Palahniuk

No abstract provided.

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The Potential For Ontogenetic Vertical Migration By Larvae Of Bathyal Echinoderms, William Jaeckle, Craig M. Young, Michael G. Devin, Suki U.K. Ekaratne, Sophie B. George Dec 1995

The Potential For Ontogenetic Vertical Migration By Larvae Of Bathyal Echinoderms, William Jaeckle, Craig M. Young, Michael G. Devin, Suki U.K. Ekaratne, Sophie B. George

William Jaeckle

Planktotrophy is a relatively common developmental mode among bathyal and abyssal echinoderms, but the sources of food used by deep-sea planktotrophic larvae remain generally unknown. Very few deep-sea echinoderm larvae have been collected in plankton samples, so we do not know whether larvae migrate to the euphotic zone to feed or if they rely on bacteria or detritus at greater depths. We approached this question indirectly by investigating whether larvae of bathyal echinoids can tolerate the temperatures they would encounter in the euphotic zone and whether they possess sufficient energy stores to migrate to the euphotic zone without feeding. Twenty-four …

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Concaveation And Maintenance Of Maternal Behavior In A Burrower Bug (Sehirus Cinctus): A Comparative Perspective, Scott Kight Dec 1995

Concaveation And Maintenance Of Maternal Behavior In A Burrower Bug (Sehirus Cinctus): A Comparative Perspective, Scott Kight

Scott Kight

This study investigates 2 patterns of maternal behavior typical of mammals, using a heterop- teran insect as the study animal. Sehirus cinctus, a burrower bug (Heteroptera: Cydnidae), exhibits relatively well-developed maternal behavior that includes guarding eggs and provi- sioning offspring. Mothers remained maternally responsive to stimulus eggs for 24-48 hr following removal of their own eggs, but the response grew weaker with longer separation times. A proportion of nulliparous females also exhibited maternal responsiveness when presented with stimulus eggs. Males, however, never responded parentally to eggs. The results of this study highlight similarities in general female responsiveness to stimulus young …

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