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Method For The Detection Of 17-B-Estradiol In Wastewater Facility Effluents Using Hplc, Alexa Mariah Fisher, Benjamin J. Thornton
Method For The Detection Of 17-B-Estradiol In Wastewater Facility Effluents Using Hplc, Alexa Mariah Fisher, Benjamin J. Thornton
Research in Biology
Studies of the effects of estrogen in aquatic ecosystems largely focus on fish. In fish, estrogen reduces fecundity in females, reduces testicular development and fertility in males, and alters vitellogenin production in both sexes. One way estrogens enter aquatic environments is via wastewater effluents. Effluent samples from the Moccasin Bend Wastewater Treatment Facility in Chattanooga, Tennessee were tested for the presence of 17-beta-estradiol (E2), using an Agilent 1260 Infinity LC (HPLC). We were not able to detect the presence of E2 in these samples with the methods described. However, when effluent samples were spiked with stock E2 (final concentration of …