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Mutations In Two Ku Homologs Define A Dna End-Joining Repair Pathway In Saccharomyces Cerevisiae, George Todd Milne, Shengfang Jin, Katie Shannon, David T. Weaver
Mutations In Two Ku Homologs Define A Dna End-Joining Repair Pathway In Saccharomyces Cerevisiae, George Todd Milne, Shengfang Jin, Katie Shannon, David T. Weaver
Biological Sciences Faculty Research & Creative Works
DNA double-strand break (DSB) repair in mammalian cells is dependent on the Ku DNA binding protein complex. However, the mechanism of Ku-mediated repair is not understood. We discovered a Saccharomyces cerevisiae gene (KU80) that is structurally similar to the 80-kDa mammalian Ku subunit. Ku80 associates with the product of the HDF1 gene, forming the major DNA end-binding complex of yeast cells. DNA end binding was absent in ku80Δ, hdf1Δ, or ku80Δ hdf1Δ strains. Antisera specific for epitope tags on Ku80 and Hdf1 were used in supershift and immunodepletion experiments to show that both proteins …