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Full-Text Articles in Biology
Telomerase Activity Is Required For The Telomere G-Overhang Structure In Trypanosoma Brucei, Ranjodh Sandhu, Bibo Li
Telomerase Activity Is Required For The Telomere G-Overhang Structure In Trypanosoma Brucei, Ranjodh Sandhu, Bibo Li
Biological, Geological, and Environmental Faculty Publications
Trypanosoma brucei causes fatal human African trypanosomiasis and evades the host immune response by regularly switching its major surface antigen, VSG, which is expressed exclusively from subtelomeric loci. Telomere length and telomere proteins play important roles in regulating VSG silencing and switching. T. brucei telomerase plays a key role in maintaining telomere length, and T. brucei telomeres terminate in a single-stranded 3′ G-rich overhang. Understanding the detailed structure of the telomere G-overhang and its maintenance will contribute greatly to better understanding telomere maintenance mechanisms. Using an optimized adaptor ligation assay, we found that most T. brucei telomere G-overhangs end in …
Trypanosoma Brucei Rap1 Maintains Telomere And Subtelomere Integrity By Suppressing Terra And Telomeric Rna: Dna Hybrids, Vishal Nanavaty, Ranjodh Sandhu, Sanaa E. Jehi, Unnati M. Pandya, Bibo Li
Trypanosoma Brucei Rap1 Maintains Telomere And Subtelomere Integrity By Suppressing Terra And Telomeric Rna: Dna Hybrids, Vishal Nanavaty, Ranjodh Sandhu, Sanaa E. Jehi, Unnati M. Pandya, Bibo Li
Biological, Geological, and Environmental Faculty Publications
Trypanosoma brucei causes human African trypanosomiasis and regularly switches its major surface antigen, VSG, thereby evading the host's immune response. VSGs are monoallelically expressed from subtelomeric expression sites (ESs), and VSG switching exploits subtelomere plasticity. However, subtelomere integrity is essential for T. brucei viability. The telomeric transcript, TERRA, was detected in T. brucei previously. We now show that the active ES-adjacent telomere is transcribed. We find that TbRAP1, a telomere protein essential for VSG silencing, suppresses VSG gene conversionmediated switching. Importantly, TbRAP1 depletion increases the TERRA level, which appears to result from longer read-through into the telomere downstream of the …
Consequences Of Dam Removal On Mussel Assemblages (Unionidae) In The Cuyahoga River, Nicholas P. Hogya, Rachel E. Andrikanich, Ryan J. Trimbath, Robert A. Krebs
Consequences Of Dam Removal On Mussel Assemblages (Unionidae) In The Cuyahoga River, Nicholas P. Hogya, Rachel E. Andrikanich, Ryan J. Trimbath, Robert A. Krebs
Biological, Geological, and Environmental Faculty Publications
The primary objective of a new survey of the Cuyahoga River was to assess species richness and population abundance of various mussel species in the family Unionidae throughout the Middle Cuyahoga River and upper parts of the Lower Cuyahoga River. Historically, few records existed for this river between Lake Rockwell and the Cuyahoga Valley National Park, a region in which four dams have been removed in the past 12 years. Timed visual surveys were conducted during low flow conditions and by using tactile techniques in deeper water. Throughout the survey, only 37 live animals were located across the 18 Middle …
Developmental Stages Identified In The Trophozoite Of The Free-Living Alveolate Flagellate Colpodella Sp. (Apicomplexa), Raghavendra Yadavalli, Tobili Y. Sam-Yellowe
Developmental Stages Identified In The Trophozoite Of The Free-Living Alveolate Flagellate Colpodella Sp. (Apicomplexa), Raghavendra Yadavalli, Tobili Y. Sam-Yellowe
Biological, Geological, and Environmental Faculty Publications
In this study we performed light, immunofluorescent and transmission electron microscopy of Colpodella trophozoites to characterize trophozoite morphology and protein distribution. The use of Giemsa staining and antibodies to distinguish Colpodella life cycle stages has not been performed previously. Rhoptry and β-tubulin antibodies were used in immunofluorescent assays (IFA) to identify protein localization and distribution in the trophozoite stage of Colpodella (ATCC 50594). We report novel data identifying “doughnut-shaped” vesicles in the cytoplasm and apical end of Colpodella trophozoites reactive with antibodies specific to Plasmodium merozoite rhoptry proteins. Giemsa staining and immunofluorescent microscopy identified different developmental stages of Colpodella trophozoites, …