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Impacts Of Environmental Stressors On Mummichog (Fundulus Heteroclitus) Growth And The Gh-Igf1 Pathway Using A Laboratory Bioassay, Olena Kuntyj
Theses and Dissertations (Comprehensive)
Using a flow-through laboratory bioassay, experiments investigated the effects of limited feeding, increased temperature, ammonium chloride (NH4Cl), and 17α-ethinylestradiol (EE2) on juvenile mummichog (Fundulus heteroclitus) growth. Weight measurements were taken every three days as the apical growth endpoint. Gene expression was examined by qPCR for genes in the GH (growth hormone)-IGF1 (insulin-like growth factor 1) pathway, including gh, igf1, and their receptors (ghra, ghrb, igf1ra, igf1rb). In Experiment 1, juvenile mummichog were exposed to excess or limited feed for 21 days. The limited-fed mummichog weighed less, were …
Mechanisms Of Nitrogenous Waste Excretion During The Complex Life Cycle Of The Sea Lamprey (Petromyzon Marinus), James M. Neal Mr.
Mechanisms Of Nitrogenous Waste Excretion During The Complex Life Cycle Of The Sea Lamprey (Petromyzon Marinus), James M. Neal Mr.
Theses and Dissertations (Comprehensive)
Sea lampreys (Petromyzon marinus) are a phylogenetically ancient jawless fish, with a multi-staged life cycle characterized by a prolonged suspension-feeding larval stage, which is followed by metamorphosis into parasitic lampreys that feed on the protein-rich blood of fishes. The switch from a nutrient poor to protein-rich diet in the sea lamprey is associated with an increased capacity to deaminate excess amino acids and to excrete ammonia and urea following metamorphosis. The focus of this thesis was to determine if changes in nitrogenous waste transporter protein abundance facilitate ammonia and urea excretion during different stages of the sea lamprey …