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Full-Text Articles in Biology

Genetic And Epigenetic Regulations Of Eds1-Mediated Plant Immunity And Identification Of Bacterial Type Iii Effectors Targeting De Novo Dna Methyltransferase Drm2 In Arabidopsis, Ming Zhao Jul 2021

Genetic And Epigenetic Regulations Of Eds1-Mediated Plant Immunity And Identification Of Bacterial Type Iii Effectors Targeting De Novo Dna Methyltransferase Drm2 In Arabidopsis, Ming Zhao

Theses and Dissertations

In Arabidopsis thaliana, Enhanced Disease Susceptibility1 (EDS1) acts as an indispensable hub in plant immunity and is mainly responsible for plant basal defense, systemic acquired resistance (SAR), increase of endogenous content of salicylic acid (SA) and effector-triggered immunity (ETI). Epigenetic regulators play crucial roles in multiple important cellular processes. As one catalytic core of Polycomb Repressive Complex (PRC2), CURLY LEAF (CLF/SDG1) protein promotes H3K27me3 deposition at specific loci for genes repression. In this work, I found that EDS1 interacted with epigenetic regulators SDG1, SDG9, SDG15, SDG22 and SDG35 in yeast two-hybrid assays. The growth of the bacterial pathogen Pseudomonas …


Regulation Of Arabidopsis Floral Organ Development By The Transcription Factor Aintegumenta-Like6, Jorman Heflin Jul 2019

Regulation Of Arabidopsis Floral Organ Development By The Transcription Factor Aintegumenta-Like6, Jorman Heflin

Theses and Dissertations

Flowers, and the fruits and seeds they produce, are of great agricultural and economic importance. Understanding the molecular mechanisms that mediate the identity, size and arrangement of floral organs within the flower may allow us to tune these elements for increased crop productivity. I examine several potential regulatory targets of the Arabidopsis AINTEGUMENTA-LIKE6 (AIL6) transcription factor that is involved in these processes and I identify protein-protein interactions that may mediate its activity in flowers. As described by the ABCE model, unique floral organ identities are specified in each whorl of the flower by different combinations of homeotic gene activities. Two …


Descriptive Analyses Of Pollen Surface Morphologies In The Model Systems Brassica Rapa And Arabidopsis Thaliana And Three Arabidopsis Pollen Wall Mutants By Scanning Electron Microscopy, Andrew B. Kirkpatrick May 2015

Descriptive Analyses Of Pollen Surface Morphologies In The Model Systems Brassica Rapa And Arabidopsis Thaliana And Three Arabidopsis Pollen Wall Mutants By Scanning Electron Microscopy, Andrew B. Kirkpatrick

Theses and Dissertations

The mechanisms behind the construction of the pollen wall are equally elaborate and mysterious. Previous studies primarily used sectioned tissue to elucidate the events involved in proper pollen development. This study proposed and evaluated a protocol for exposing developing microspores to be examined by Scanning Electron Microscopy (SEM). Utilizing this protocol, comparative analyses of the superficial features present at the early, middle, and late tetrad as well as at released microspore stages of the model plants Brassica rapa and Arabidopsis thaliana were conducted. The utility of the technique was then evaluated through the examination of three Arabidopsis pollen wall mutants …


Ferric Reductases And Transporters That Contribute To Mitochondrial Iron Homeostasis, Anshika Jain Dec 2014

Ferric Reductases And Transporters That Contribute To Mitochondrial Iron Homeostasis, Anshika Jain

Theses and Dissertations

Iron (Fe) is the fourth most abundant element in the Earth’s crust, yet the availability of Fe to plants is often limited. This is because in most soil types, Fe precipitates as ferric-oxyhydroxy complexes, making it unavailable for uptake by plants. While the mechanisms involved in Fe uptake from the soil are relatively well understood, the mechanisms involved in its further distribution to the aerial portion of the plant and to subcellular compartments are not fully understood. During Fe deprivation, plants up-regulate root Fe acquisition machinery. How plants sense Fe deprivation and tie the Fe status of the plant to …