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Expression Of Gene Product 126 From Phage Gizmo And Creation Of A Substrate, Manish Mandava, David Bollivar, Faculty Advisor
Expression Of Gene Product 126 From Phage Gizmo And Creation Of A Substrate, Manish Mandava, David Bollivar, Faculty Advisor
John Wesley Powell Student Research Conference
Endonucleases are enzymes which cleave DNA at specific nucleotide sequences. Homing Endonucleases (HEase) are a group of endonucleases that reside as open reading frames within self-splicing introns or inteins. After expression of the gene containing the HEase the HEase goes on to cleave a target site in a homolog of the hosting gene to induce homologous recombination turning the vacant homolog into a homing endonuclease gene (HEG). It is predicted that gene product (gp126) from the mycobacteriophage Gizmo encodes a HEase. To test whether the gene encodes a HEase the phage sequence of gp126 Gizmo was transferred into the his-tag …
Creating And Implementing A Teaching Module For The High School Biology Classroom, Elizabeth Smith, William Jaeckle, Faculty Advisor
Creating And Implementing A Teaching Module For The High School Biology Classroom, Elizabeth Smith, William Jaeckle, Faculty Advisor
John Wesley Powell Student Research Conference
The “nature of science” is an educational concept that encourages science teachers to teach students about the origin, nature, methods, and limits of human knowledge. One way for students to experience the nature of science is through the creation and implementation of hands-on experiments. As a future biology teacher, I created a teaching module that incorporates an inquiry-based experiment that could be implemented into a high school biology curriculum. In this experiment, egg masses from the locally common freshwater snail Physa acuta were used to test the effect of rearing temperature on the rate of embryo development and survival. Within …