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- Chaetotaxy (2)
- Drug repurposing (2)
- Trichomonas vaginalis (2)
- 4-aminoquinoline-hybridization (1)
- Antifungal intervention (1)
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- Antimalarial agents (1)
- Antimicrobial agents (1)
- Antimicrobial drug resistance (1)
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- Bella (1)
- Benzoxaboroles (1)
- Bleuzeni (1)
- Brunfelsia grandiflora (1)
- Caryodendron (1)
- Chalcothea (1)
- Chemosensitization (1)
- Cinnamic acids (1)
- Columbidae (1)
- Comparative genomics (1)
- Conjugate acid-base pairs (1)
- Divalis (1)
- Drug resistance; mutants (1)
- Ecuador (1)
- Egg (1)
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Articles 1 - 21 of 21
Full-Text Articles in Biology
High Efficiency Drug Repurposing Design For New Antifungal Agents, Jong H. Kim, Kathleen L. Chan, Luisa W. Cheng, Lisa A. Tell, Barbara A. Byrne, Kristin Clothier, Kirkwood M. Land
High Efficiency Drug Repurposing Design For New Antifungal Agents, Jong H. Kim, Kathleen L. Chan, Luisa W. Cheng, Lisa A. Tell, Barbara A. Byrne, Kristin Clothier, Kirkwood M. Land
College of the Pacific Faculty Articles
Current antifungal interventions have often limited efficiency in treating fungal pathogens, particularly those resistant to commercial drugs or fungicides. Antifungal drug repurposing is an alternative intervention strategy, whereby new utility of various marketed, non-antifungal drugs could be repositioned as novel antifungal agents. In this study, we investigated "chemosensitization" as a method to improve the efficiency of antifungal drug repurposing, wherein combined application of a second compound (viz., chemosensitizer) with a conventional, non-antifungal drug could greatly enhance the antifungal activity of the co-applied drug. Redox-active natural compounds or structural derivatives, such as thymol (2-isopropyl-5-methylphenol), 4-isopropyl-3-methylphenol, or 3,5-dimethoxybenzaldehyde, could serve as potent …
Highly Potent 1h-1,2,3-Triazole-Tethered Isatin-Metronidazole Conjugates Against Anaerobic Foodborne, Waterborne, And Sexually-Transmitted Protozoal Parasites, Sumit Kumar, Trpta Bains, Ashley Sae Won Kim, Christina Tam, Jong Kim, Luisa W. Cheng, Kirkwood M. Land, Anjan Debnath, Vipan Kumar
Highly Potent 1h-1,2,3-Triazole-Tethered Isatin-Metronidazole Conjugates Against Anaerobic Foodborne, Waterborne, And Sexually-Transmitted Protozoal Parasites, Sumit Kumar, Trpta Bains, Ashley Sae Won Kim, Christina Tam, Jong Kim, Luisa W. Cheng, Kirkwood M. Land, Anjan Debnath, Vipan Kumar
College of the Pacific Faculty Articles
Parasitic infections like amebiasis, trichomoniasis, and giardiasis are major health threats in tropical and subtropical regions of the world. Metronidazole (MTZ) is the current drug of choice for amebiasis, giardiasis, and trichomoniasis but it has several adverse effects and potential resistance is a concern. In order to develop alternative antimicrobials, a library of 1H-1,2,3-triazole-tethered metronidazole-isatin conjugates was synthesized using Huisgen's azide-alkyne cycloaddition reaction and evaluated for their amebicidal, anti-trichomonal, and anti-giardial potential. Most of the synthesized conjugates exhibited activities against Trichomonas vaginalis, Tritrichomonas foetus, Entamoeba histolytica, and Giardia lamblia. While activities against T. vaginalis and T. foetus were comparable to …
Cinnamoyl- Oxaborole Amides: Synthesis And Their In Vitro Biological Activity, Maureen Gumbo, Richard M. Beteck, Tawanda Mandizvo, Ronnett Seldon, Digby F. Warner, Heinrich C. Hoppe, Michelle Isaacs, Dustin Laming, Christina C. Tam, Luisa W. Cheng, Nicole Liu, Kirkwood M. Land, Setshaba D. Khanye
Cinnamoyl- Oxaborole Amides: Synthesis And Their In Vitro Biological Activity, Maureen Gumbo, Richard M. Beteck, Tawanda Mandizvo, Ronnett Seldon, Digby F. Warner, Heinrich C. Hoppe, Michelle Isaacs, Dustin Laming, Christina C. Tam, Luisa W. Cheng, Nicole Liu, Kirkwood M. Land, Setshaba D. Khanye
College of the Pacific Faculty Articles
Due to the increased interest in their application in the treatment of infectious diseases, boron-containing compounds have received a significant coverage in the literature. Herein, a small set of novel cinnamoly-oxaborole amides were synthesized and screened against nagana Trypanosoma brucei brucei for antitrypanosomal activity. Compound 5g emerged as a new hit with an in vitro IC50 value of 0.086 μM against T. b. brucei without obvious inhibitory activity against HeLa cell lines. The same series was also screened against other human pathogens, including Mycobacterium tuberculosis, the causative agent of tuberculosis (TB), for which moderate to weak activity (10 to >125 …
Synthesis And Preliminary Antimicrobial Analysis Of Isatin- Ferrocene And Isatin-Ferrocenyl Chalcone Conjugates, Amandeep Singh, Grant Fong, Jenny Liu, Yun-Hsuan Wu, Kevin Chang, William Park, Jihwan Kim, Christina Tam, Luisa W. Cheng, Kirkwood M. Land, Vipan Kumar
Synthesis And Preliminary Antimicrobial Analysis Of Isatin- Ferrocene And Isatin-Ferrocenyl Chalcone Conjugates, Amandeep Singh, Grant Fong, Jenny Liu, Yun-Hsuan Wu, Kevin Chang, William Park, Jihwan Kim, Christina Tam, Luisa W. Cheng, Kirkwood M. Land, Vipan Kumar
College of the Pacific Faculty Articles
In this study, we outline the synthesis of isatin-ferrocenyl chalcone and 1H-1,2,3-triazole-tethered isatin-ferrocene conjugates along with their antimicrobial evaluation against the human mucosal pathogen Trichomonas vaginalis. The introduction of a triazole ring among the synthesized conjugates improved the activity profiles with most of the compounds in the library, exhibiting 100% growth inhibition in a preliminary susceptibility screen at 100 μM. IC50 determination of the most potent compounds in the set revealed an inhibitory range between 2 and 13 μM. Normal flora microbiome are unaffected by these compounds, suggesting that these may be new chemical scaffolds for the discovery of new …
High-Throughput Screen Of Drug Repurposing Library Identifies Inhibitors Of Sarcocystis Neurona Growth, Gregory D. Bowden, Kirkwood M. Land, Roberta M. O'Connor, Heather M. Fritz
High-Throughput Screen Of Drug Repurposing Library Identifies Inhibitors Of Sarcocystis Neurona Growth, Gregory D. Bowden, Kirkwood M. Land, Roberta M. O'Connor, Heather M. Fritz
College of the Pacific Faculty Articles
The apicomplexan parasite Sarcocystis neurona is the primary etiologic agent of equine protozoal myeloencephalitis (EPM), a serious neurologic disease of horses. Many horses in the U.S. are at risk of developing EPM; approximately 50% of all horses in the U.S. have been exposed to S. neurona and treatments for EPM are 60-70% effective. Advancement of treatment requires new technology to identify new drugs for EPM. To address this critical need, we developed, validated, and implemented a high-throughput screen to test 725 FDA-approved compounds from the NIH clinical collections library for anti-S. neurona activity. Our screen identified 18 compounds with confirmed …
An Unconventional Collaboration At The College Level To Improve Stem Student Success, Amy N. Scott, Eileen Kogl Camfield, Alex Miller, Kirkwood M. Land
An Unconventional Collaboration At The College Level To Improve Stem Student Success, Amy N. Scott, Eileen Kogl Camfield, Alex Miller, Kirkwood M. Land
College of the Pacific Faculty Articles
The purpose of this article is to illustrate the work that has resulted from a collaboration between a biology professor, a school psychology professor, a researcher in higher education access, and the writing programs director. The essential school psychologist role, as classroom observer and data analyzer, is discussed through an example of work done as part of a larger project focusing on student success and retention for at-risk populations in introductory college biology courses. Best practices for consulting at the college level are discussed and include: collaborate to cultivate the willing, collect and analyze data to sustain instructor involvement, and …
Phytochemical-Rich Foods Inhibit The Growth Of Pathogenic Trichomonads, Sabrina M. Noritake, Jenny Liu, Sierra Kanetake, Carol E. Levin, Christina Tam, Luisa W. Cheng, Kirkwood M. Land, Mendel Friedman
Phytochemical-Rich Foods Inhibit The Growth Of Pathogenic Trichomonads, Sabrina M. Noritake, Jenny Liu, Sierra Kanetake, Carol E. Levin, Christina Tam, Luisa W. Cheng, Kirkwood M. Land, Mendel Friedman
College of the Pacific Faculty Articles
Plants produce secondary metabolites that often possess widespread bioactivity, and are then known as phytochemicals. We previously determined that several phytochemical-rich food-derived preparations were active against pathogenic foodborne bacteria. Trichomonads produce disease (trichomoniasis) in humans and in certain animals. Trichomonads are increasingly becoming resistant to conventional modes of treatment. It is of interest to test bioactive, natural compounds for efficacy against these pathogens.
Genetic Indicators Of Drug Resistance In The Highly Repetitive Genome Of Trichomonas Vaginalis, Marina Bradic, Sally D. Warring, Grace E. Tooley, Paul Scheid, William E. Secor, Kirkwood M. Land, Po-Jung Huang, Ting-Wen Chen, Chi-Ching Lee, Petrus Tang, Steven A. Sullivan, Jane M. Carlton
Genetic Indicators Of Drug Resistance In The Highly Repetitive Genome Of Trichomonas Vaginalis, Marina Bradic, Sally D. Warring, Grace E. Tooley, Paul Scheid, William E. Secor, Kirkwood M. Land, Po-Jung Huang, Ting-Wen Chen, Chi-Ching Lee, Petrus Tang, Steven A. Sullivan, Jane M. Carlton
College of the Pacific Faculty Articles
Trichomonas vaginalis, the most common nonviral sexually transmitted parasite, causes ∼283 million trichomoniasis infections annually and is associated with pregnancy complications and increased risk of HIV-1 acquisition. The antimicrobial drug metronidazole is used for treatment, but in a fraction of clinical cases, the parasites can become resistant to this drug. We undertook sequencing of multiple clinical isolates and lab derived lines to identify genetic markers and mechanisms of metronidazole resistance. Reduced representation genome sequencing of ∼100 T. vaginalis clinical isolates identified 3,923 SNP markers and presence of a bipartite population structure. Linkage disequilibrium was found to decay rapidly, suggesting genome-wide …
4-Aminoquinoline-Hybridization En Route Towards The Development Of Rationally Designed Antimalarial Agents, Raghu Raj, Kirkwood M. Land, Vipan Kumar
4-Aminoquinoline-Hybridization En Route Towards The Development Of Rationally Designed Antimalarial Agents, Raghu Raj, Kirkwood M. Land, Vipan Kumar
College of the Pacific Faculty Articles
The resistance of Plasmodium falciparum, the causative agent of malaria, against quinine and chloroquine along with the lack of malaria vaccines has encouraged the development of various synthetic strategies towards biologically active scaffolds. An emerging strategy in medicinal chemistry, termed molecular hybridization, involves the covalent fusion of two or more drugs, active compounds, and/or pharmacophoric units into a hybrid compound, with fascinating activities and multiple but not essentially simultaneous pharmacological targets. 4-Aminoquinolines are considered as promising antimalarials and 4-aminoquinoline hybridization is considered as an attractive and feasible approach for the development of new molecular frameworks for averting and delaying the …
Trichomonas Stableri N. Sp., An Agent Of Trichomonosis In Pacific Coast Band-Tailed Pigeons (Patagioenas Fasciata Monilis), Yvette A. Girard, Krysta H. Rogers, Richard Gerhold, Kirkwood M. Land, Scott C. Lenaghan, Leslie W. Woods, Nathan Haberkern, Melissa Hopper, Jeff D. Cann, Christine K. Johnson
Trichomonas Stableri N. Sp., An Agent Of Trichomonosis In Pacific Coast Band-Tailed Pigeons (Patagioenas Fasciata Monilis), Yvette A. Girard, Krysta H. Rogers, Richard Gerhold, Kirkwood M. Land, Scott C. Lenaghan, Leslie W. Woods, Nathan Haberkern, Melissa Hopper, Jeff D. Cann, Christine K. Johnson
College of the Pacific Faculty Articles
Trichomonas gallinae is a ubiquitous flagellated protozoan parasite, and the most common etiologic agent of epidemic trichomonosis in columbid and passerine species. In this study, free-ranging Pacific Coast band-tailed pigeons (Patagioenas fasciata monilis) in California (USA) were found to be infected with trichomonad protozoa that were genetically and morphologically distinct from T. gallinae. In microscopic analysis, protozoa were significantly smaller in length and width than T. gallinae and were bimodal in morphology. Phylogenetic analysis of the ITS1/5.8S/ITS2, rpb1, and hydrogenosomal Fe-hydrogenase regions revealed that the protozoan shares an ancestor with Trichomonas vaginalis, the sexually-transmitted agent of trichomoniasis in humans. Clinical …
The Immature Stages And Natural History Of Veladyris Pardalis (Salvin, 1869) In Eastern Ecuador (Lepidoptera, Nymphalidae, Ithomiinae), H. F. Greeney, Ryan I. Hill, M. R. Hualinga, W. R. Simbana, G. Gentry
The Immature Stages And Natural History Of Veladyris Pardalis (Salvin, 1869) In Eastern Ecuador (Lepidoptera, Nymphalidae, Ithomiinae), H. F. Greeney, Ryan I. Hill, M. R. Hualinga, W. R. Simbana, G. Gentry
College of the Pacific Faculty Articles
We describe the immature stages and oviposition behavior of Veladyris pardalis (Salvin, 1869) from northeastern Ecuador. An unidentified species of Solanum (Solanaceae) is the larval food plant. Eggs are laid singly on leaves, stems or epiphytes growing on the host. Veladyris pardalis has four larval stadia, and takes 64–70 days to mature from oviposition to adult.
Promoter Library Designed For Fine-Tuned Gene Expression In Pichia Pastoris, Franz S. Hartner, Claudia Ruth, David Langenegger, Sabrina N. Johnson, Petr Hyka, Geoff P. Lin-Cereghino, Joan Lin-Cereghino, Karin Kovar, James Cregg, Anton Glieder
Promoter Library Designed For Fine-Tuned Gene Expression In Pichia Pastoris, Franz S. Hartner, Claudia Ruth, David Langenegger, Sabrina N. Johnson, Petr Hyka, Geoff P. Lin-Cereghino, Joan Lin-Cereghino, Karin Kovar, James Cregg, Anton Glieder
College of the Pacific Faculty Articles
Although frequently used as protein production host, there is only a limited set of promoters available to drive the expression of recombinant proteins in Pichia pastoris. Fine-tuning of gene expression is often needed to maximize product yield and quality. However, for efficient knowledge-based engineering, a better understanding of promoter function is indispensable. Consequently, we created a promoter library by deletion and duplication of putative transcription factor-binding sites within the AOX1 promoter (PAOX1) sequence. This first library initially spanned an activity range between ∼6% and >160% of the wild-type promoter activity. After characterization of the promoter library employing …
Description Of The Immature Stages Of Methona Confusa Confusa Butler, 1873 And Methona Curvifascia Weymer, 1883 (Nymphalidae, Ithomiinae) From Eastern Ecuador, Ryan I. Hill, Luis A. Tipan
Description Of The Immature Stages Of Methona Confusa Confusa Butler, 1873 And Methona Curvifascia Weymer, 1883 (Nymphalidae, Ithomiinae) From Eastern Ecuador, Ryan I. Hill, Luis A. Tipan
College of the Pacific Faculty Articles
Here we describe the complete life history for Methona confusa confusa Butler, 1873 and Methona curvifascia Weymer, 1883 from eastern Ecuador. Each stage from egg to pupa is described and illustrated. Descriptions of first instar chaetotaxy and instar durations are also reported. Both species were found feeding on Brunfelsia grandiflora schultesii Plowman. Mature M. confusa larvae have 12 transverse bands that are all yellow in color, including one on segment A9 as observed for M. megisto and M. themisto. In contrast, M. curvifascia lacks a transverse band on segment A9, having 11 transverse bands in total that are white in …
Life History And Biology Of Forbestra Olivencia (Ithomiinae, Nymphalidae), Ryan I. Hill
Life History And Biology Of Forbestra Olivencia (Ithomiinae, Nymphalidae), Ryan I. Hill
College of the Pacific Faculty Articles
Forbestra is the only mechanitine genus lacking a thorough life history description and little is known of its biology. Accordingly I describe the immature stages including first instar chaetotaxy, and provide observations on the biology of Forbestra olivencia from Garza Cocha in eastern Ecuador. Morphological characters from the early stages of Forbestra olivencia are identified that are unique to Forbestra and support the close relationship of Forbestra and Mechanitis. Forbestra olivencia was a moderately common butterfly at Garza Cocha during the sample period, far outnumbering other sympatric Forbestra. Ecological observations demonstrate similarities between F. olivencia and Mechanitis, but suggest F. …
Diet: Charina Bottae, T. J. Devitt, Ryan I. Hill, S. E. Cameron
Diet: Charina Bottae, T. J. Devitt, Ryan I. Hill, S. E. Cameron
College of the Pacific Faculty Articles
No abstract provided.
Condensed Protocol For Competent Cell Preparation And Transformation Of The Methylotrophic Yeast Pichia Pastoris, Joan Lin-Cereghino, William Wong, See Xiong, William Giang, Linda Luong, Jane Vu, Sabrina Johnson, Geoff P. Lin-Cereghino
Condensed Protocol For Competent Cell Preparation And Transformation Of The Methylotrophic Yeast Pichia Pastoris, Joan Lin-Cereghino, William Wong, See Xiong, William Giang, Linda Luong, Jane Vu, Sabrina Johnson, Geoff P. Lin-Cereghino
College of the Pacific Faculty Articles
No abstract provided.
Temperature-Dependent Oxygen Limitation In Insect Eggs, H. Arthur Woods, Ryan I. Hill
Temperature-Dependent Oxygen Limitation In Insect Eggs, H. Arthur Woods, Ryan I. Hill
College of the Pacific Faculty Articles
Most terrestrial insect embryos support metabolism with oxygen from the environment by diffusion across the eggshell. Because metabolism is more temperature sensitive than diffusion, embryos should be relatively oxygen-limited at high temperatures. We tested whether survival, development time and metabolism of eggs of a moth, Manduca sexta, were sensitive to experimentally imposed variation in atmospheric oxygen availability (5–50 kPa; normoxia at sea level is 21 kPa) across a range of biologically realistic temperatures. Temperature–oxygen interactions were apparent in most experiments. Hypoxia affected survival more strongly at warmer temperatures. Metabolic rates, measured as rates of CO2 emission, were virtually insensitive …
Notes On The Life History Of Asterope Markii Hewitson, 1857 (Nymphalidae), Ryan I. Hill
Notes On The Life History Of Asterope Markii Hewitson, 1857 (Nymphalidae), Ryan I. Hill
College of the Pacific Faculty Articles
No abstract provided.
Phylogenetic Analysis And Review Of Panacea And Batesia Butterflies (Nymphalidae), Ryan I. Hill, Carla M. Penz, P. J. Devries
Phylogenetic Analysis And Review Of Panacea And Batesia Butterflies (Nymphalidae), Ryan I. Hill, Carla M. Penz, P. J. Devries
College of the Pacific Faculty Articles
Phylogenetic analysis of 53 morphological characte rs for five species of Panacea and Batesia hypochlora supports the separation of the two genera and showed that the monotypic genus Batesia is basal to Panacea. Male genitalia were Ilniform within Panacea and characters inir)f)llative for phylogeny reconstruction were restricted to wing coloration. Illustrations of adults and genitalia, a brief diagnosis, and distributions are provided le)r each species.
Endosome To Golgi Retrieval Of The Vacuolar Protein Sorting Receptor, Vps10p, Requires The Function Of The Vps29, Vps30, And Vps35 Gene Products, Matthew N. J. Seaman, Eric G. Marcusson, Joan Lin-Cereghino, Scott D. Emr
Endosome To Golgi Retrieval Of The Vacuolar Protein Sorting Receptor, Vps10p, Requires The Function Of The Vps29, Vps30, And Vps35 Gene Products, Matthew N. J. Seaman, Eric G. Marcusson, Joan Lin-Cereghino, Scott D. Emr
College of the Pacific Faculty Articles
Mutations in the S. cerevisiae VPS29 and VPS30 genes lead to a selective protein sorting defect in which the vacuolar protein carboxypeptidase Y (CPY) is missorted and secreted from the cell, while other soluble vacuolar hydrolases like proteinase A (PrA) are delivered to the vacuole. This phenotype is similar to that seen in cells with mutations in the previously characterized VPS10 and VPS35 genes. Vps10p is a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases like CPY and PrA, while Vps35p is a peripheral membrane protein which cofractionates with membranes enriched in Vps10p. The …
The Cytoplasmic Tail Domain Of The Vacuolar Protein Sorting Receptor Vps10p And A Subset Of Vps Gene Products Regulate Receptor Stability, Function, And Localization, Joan Lin-Cereghino, Eric G. Marcusson, Scott D. Emr
The Cytoplasmic Tail Domain Of The Vacuolar Protein Sorting Receptor Vps10p And A Subset Of Vps Gene Products Regulate Receptor Stability, Function, And Localization, Joan Lin-Cereghino, Eric G. Marcusson, Scott D. Emr
College of the Pacific Faculty Articles
VPS10 of Saccharomyces cerevisiae encodes a type I transmembrane receptor protein required for the sorting of the soluble vacuolar hydrolase carboxypeptidase Y (CPY). To characterize the essential structural features and intercompartmental transport itinerary of the CPY receptor, we have constructed mutant forms of Vps10p that alter the carboxyterminal cytoplasmic tail of the protein. In addition, we have analyzed the effect these mutations as well as mutations in several VPS genes have on the function, stability, and localization of Vps10p. Although wild-type Vps10p is very stable over a 3-h chase period, overproduction of Vps10p results in PEP4-dependent degradation of the receptor. …