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Articles 1 - 13 of 13
Full-Text Articles in Biology
Comparative Study Of Hpv 16 And Hpv 18 Antibody Detection In Serum, Cervical Mucus, And Oral Mucosal Transudate, Emily Lauren Blalock
Comparative Study Of Hpv 16 And Hpv 18 Antibody Detection In Serum, Cervical Mucus, And Oral Mucosal Transudate, Emily Lauren Blalock
Biology Theses
Measuring HPV exposure relies on detection of HPV type-specific antibodies, but methods are not standardized. Additionally, there is little information on the best sample type for HPV antibody detection. This study validated pseudovirion neutralization (PVN) assay for HPV antibody detection and compared it to IgG ELISA. Both assays were applied to paired serum and cervical mucus samples. Additionally, PVN assay was utilized to evaluate the feasibility of oral mucosal transudate (OMT) samples to monitor the HPV immune response. Serum was more likely to be positive on PVN assay than on IgG ELISA (p= 0.025). Both assays correlated with HPV-16 DNA …
The Production Of Designed Potential Protein Contrast Agents And Their Encapsulation In Albumin Microspheres, Julian A. Johnson
The Production Of Designed Potential Protein Contrast Agents And Their Encapsulation In Albumin Microspheres, Julian A. Johnson
Biology Theses
Using protein design, a series of metal binding proteins have been designed, allowing the local factors that contribute to metal affinity and thermostability to be studied. Those proteins with the highest metal binding affinities had the lowest apo-form Tm and the largest ÄTm upon metal binding. In this thesis, major steps have been taken toward applying the engineered protein to MR imaging. The progress of magnetic resonance imaging is hindered by low specificity and rapid elimination of FDA-approved MRI contrast agents. The engineered protein contrast agent has been conjugated to a cancer-specific targeting peptide and encapsulated in albumin microspheres to …
The Effects Of An Artifically Elevated Thermal Environment And Seasonal Acclimatization On The Thermal Tolerance Of The Western Mosquitofish, Gambusia Affinis, Rebbekah Watson
Biology Theses
Gambusia affinis, the western mosquitofish, is a very hardy, live-bearing fish that has invaded freshwater habitats worldwide. The success of the western mosquitofishs' invasion is due to its reproductive capabilities and ability to tolerate a wide range of temperatures. The mosquitofish can inhabit waters as low as 0°C and higher than 40°C. Because of this species' eurythermicity, questions have been raised regarding the impact of artificially heated environments on its thermal tolerance limits and whether its thermal tolerance limits are consistent across seasons. Past studies have shown that mosquitofish populations inhabiting hot ponds receiving thermal effluents at steam-electric power stations …
Production Of B Virus Glycoprotein D And Evaluation Of Its Diagnostic Potential, Chadi N. Filfili
Production Of B Virus Glycoprotein D And Evaluation Of Its Diagnostic Potential, Chadi N. Filfili
Biology Theses
B virus diagnosis presents a challenge largely complicated by the asymptomatic infection of rhesus macaques, and extremely pathogenic fatal infections in humans. Humoral detection of antibodies is generally performed using whole virus antigen for which preparation requires strict biosafety measures and specialized BSL-4 facilities. As an alternative to utilizing B virus antigen, we describe the production of a truncated form of B virus envelope glycoprotein D, gD 287, in a baculovirus expression system, and evaluate its diagnostic potential as an antigen in recombinant ELISA. After purification and characterization, gD 287 was tested using 22 negative and 72 positive macaque sera …
Proteasomal Gene Duplications And Recruitment Of Testis Specific Expression In Drosophila, Mehran Sorourian
Proteasomal Gene Duplications And Recruitment Of Testis Specific Expression In Drosophila, Mehran Sorourian
Biology Theses
Proteasome is a large multisubunit complex that degrades ubiquitinated proteins in a highly regulated manner in all eukaryotes. Two subcompartments of this subunit are the 19Scap and the 20S core particle with 19S acting as regulatory subunit, it attaches at both ends of the 20Score particle harboring the catalytic domains. In D. melanogaster one third of the genes encoding for this subunit were recognized to have many male specific duplicates. In this work, we make use of the new 12 genomes sequences and show that the majority of the genes encoding for the 20S particle gave rise to at least …
The Relationship Of Oribatid Soil Mite Abundance To Abiotic And Biotic Factors, Carolyn Hess
The Relationship Of Oribatid Soil Mite Abundance To Abiotic And Biotic Factors, Carolyn Hess
Biology Theses
Oribatid soil mites primarily feed on fungi and dead organisms. They belong to the arthropod subclass, Acari, that are believed to contribute to soil processes such as plant litter breakdown and nutrient release. In order to facilitate more accurate predictions about obscure soil processes, research has been done that has attempted to correlate soil mite abundance and diversity to more easily measured aboveground biota. The present study examined monthly Oribatid soil mite abundance and its relationship to four different plant communities in North Texas: mowed grass monoculture, un-managed field, riparian, and woodland, during one growing season. Due to the nutritional …
Carbon, Nitrogen And Phosphorus Stoichiometry Of A Mixotrophic Protist, Natalie C. Lukomski
Carbon, Nitrogen And Phosphorus Stoichiometry Of A Mixotrophic Protist, Natalie C. Lukomski
Biology Theses
The microbial loop is one of two major pathways in aquatic systems in which nutrient and energy flow from dissolved nutrients to higher trophic levels. Protozoan-bacteria predator-prey relationships lie at the base of this food web. It is through this interaction that dissolved nutrients sequestered in the bacterial biomass become available to higher trophic levels. Heterotrophic nanoflagellates are the main consumers of bacteria in aquatic systems (Porter et al.1985). Consequently, limitation of dissolved nutrients in the bacterial biomass could effect protozoan stoichiometry and the amounts of excreted waste products. These excreted waste products are a valuable resource to bacteria; therefore …
The C-Phycocyanin/Beta Protein Inhibits Cancer Cell Proliferation, Haizhen Wang
The C-Phycocyanin/Beta Protein Inhibits Cancer Cell Proliferation, Haizhen Wang
Biology Theses
C-Phycocyanin (C-PC) from blue-green algae has been reported to have various pharmacological characteristics, including anti-inflammatory and anti-cancer activities. In this study, the beta-subunit of C-PC (ref to as C-PC/beta) was expressed and purified from bacteria E. coli BL-21. The recombinant C-PC/beta has been demonstrated to have anticancer properties. Under the treatment of 5 microM of the recombinant C-PC/beta, four different cancer cell lines accrued a high proliferation inhibition and apoptotic induction. The C-PC/beta interacts with membrane-associated beta-tubulin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been found. Under the treatment of the C-PC/beta, depolymerization of microtubulin and actin-filament was observed. The cells underwent …
Mapping And Filling Metabolic Pathway Holes, Dipendra Kaur
Mapping And Filling Metabolic Pathway Holes, Dipendra Kaur
Biology Theses
The network-mapping tool integrated with protein database search can be used for filling pathway holes. A metabolic pathway under consideration (pattern) is mapped into a known metabolic pathway (text), to find pathway holes. Enzymes that do not show up in the pattern may be a hole in the pattern pathway or an indication of alternative pattern pathway. We present a data-mining framework for filling holes in the pattern metabolic pathway based on protein function, prosite scan and protein sequence homology. Using this framework we suggest several fillings found with the same EC notation, with group neighbors (enzymes with same EC …
Reinforcing Efficacy Of Amphetamine In Adolescent And Adult Male Rats, Lauren Chantel Payne
Reinforcing Efficacy Of Amphetamine In Adolescent And Adult Male Rats, Lauren Chantel Payne
Biology Theses
Rationale: Amphetamine abuse by adolescents predicts long-term drug dependence. Heightened vulnerability to drug abuse could be due to higher sensitivity to drug’s reinforcing effects. Rodents are used to study age-related sensitivities to drugs. Objective: We compared intravenous amphetamine self-administration between adolescent and adult male rats on an operant schedule of reinforcement measuring the reinforcing efficacy of a drug. Methods: After surgery, adolescent and adult rats acquired lever-pressing behavior reinforced by amphetamine infusions. Results: Both age groups exhibited more infusions per session as dose increased. However, neither the number of infusions per session nor total amphetamine intake differed across age groups. …
Lack Of Respiratory Effects Of Nicotine Applied To The Caudal Medullary Raphã©, Nicole Bates
Lack Of Respiratory Effects Of Nicotine Applied To The Caudal Medullary Raphã©, Nicole Bates
Biology Theses
Breathing is an involuntary and automatic process initiated by a network of neurons within the brainstem. These neurons are affected by a number of factors (mechanical, electrical and chemical) that help modulate the frequency and pattern of breathing. Three respiratory chemosensitive sites have traditionally been described on the ventral medullary surface. These sites are affected by the pH of the cerebrospinal fluid (CSF) bathing the brain and by chemicals dissolved within the CSF. More recently, additional sites have been described with chemosensitive activity and of these the medullary raphé has received the most attention. Although the dangers of smoking is …
Transposon Exaptation In Mammalian Evolution, Donald Hucks
Transposon Exaptation In Mammalian Evolution, Donald Hucks
Biology Theses
A growing body of work suggests that the exaptation of transposon-derived sequences to perform beneficial cellular functions has played a significant role in eukaryotic evolution. In chapter 1, we present an analysis of 10 exapted pogo transposons in the human genome. We present evidence that all 10 are restricted to tetrapods, and that 8 of the 10 arose early in mammalian evolution, in several independent exaptation events involving diverse pogo lineages. We show that all 10 have been subject to stringent selection throughout mammalian evolution, with pseudogenization having occurred only infrequently. In 4 of these genes, we observed no cases …
Effects Of Nutrient Limitation, Competitors And Grazers On The Abundance And Toxicity Of Laboratory Cultures Of P. Parvum, A Harmful Alga, Ratheesh Ramachandrannair Marunnamkattil
Effects Of Nutrient Limitation, Competitors And Grazers On The Abundance And Toxicity Of Laboratory Cultures Of P. Parvum, A Harmful Alga, Ratheesh Ramachandrannair Marunnamkattil
Biology Theses
P. parvum Carter is a haptophyte that occurs world wide in both fresh and brackish waters. Though P. parvum blooms occur world wide under a variety of environmental conditions, it occurs during the cooler months of the year in Texas. Various treatment combinations of temperatures, salinity and limiting nutrients were used to test the hypothesis that the interaction of these factors would affect the abundance and toxicity of P. parvum. In addition to testing various above mentioned factors and their interactions, P. parvum was grown in mixed species cultures with major competitors from a natural lake community (Lake Whitney) to …