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Estrogen-Dependent Expression And Subcellular Localization Of The Tight Junction Protein Claudin-4 In Hec-1a Endometrial Cancer Cells, M. E. Cuevas, J. M. Gaska, A. C. Gist, Jonathan M. King, R. A. Sheller, M. C. Todd
Estrogen-Dependent Expression And Subcellular Localization Of The Tight Junction Protein Claudin-4 In Hec-1a Endometrial Cancer Cells, M. E. Cuevas, J. M. Gaska, A. C. Gist, Jonathan M. King, R. A. Sheller, M. C. Todd
Biology Faculty Research
Endometrial cancer is the most common female reproductive cancer in the United States and is associated with deregulated tight junction protein expression. Given the highly estrogen-responsive nature of this tissue, we investigated the effects of estrogen and its agonist, 4-OH TAM, on the expression and subcellular localization of the tight junction protein claudin-4 (CLDN-4), in HEC-1A endometrial cancer cells. In untreated HEC-1A cells, we observed dramatic overexpression of claudin-4 protein. In addition, differential detergent extraction analysis indicated that claudin-4 was localized primarily in the membrane but also found in the cytosolic, nuclear and cytoskeletal fractions. Upon exposure of HEC-1A to …