Biology Commons^™

Aerobic Respiration And Its Regulation In The Metal Reducer Shewanella Oneidensis, Kristen Bertling, Areen Banerjee, Daad Saffarini

Biological Sciences Faculty Articles

Shewanella oneidensis MR-1 is a facultative anaerobe known for its ability to reduce metal oxides. Anaerobic respiration, especially metal reduction, has been the subject of extensive research. In contrast, S. oneidensis aerobic respiration has received less attention. S. oneidensis expresses cbb3- and aa3-type cytochrome c oxidases and a bd-type quinol oxidase. The aa3-type oxidase, which in other bacteria is the major oxygen reductase under oxygen replete conditions, does not appear to contribute to aerobic respiration and growth in S. oneidensis. Our results indicated that although the aa3-type oxidase does not play a role in aerobic growth …

Effects Of Land Use And Pollution Loadings On Ecotoxicological Assays And Bacterial Taxonomical Diversity In Constructed Wetlands, Subhomita Ghosh Roy, Charles F. Wimpee, Stephen Andrew Mcguire, Timothy J. Ehlinger

Biological Sciences Faculty Articles

Freshwater ecosystems are affected by anthropogenic alterations. Different studies have extensively studied the concentrations of metals, nutrients, and water quality as measurements of pollution in freshwater ecosystems. However, few studies have been able to link these pollutants to bioindicators as a risk assessment tool. This study aimed to examine the potential of two bioindicators, plant ecotoxicological assays and sediment bacterial taxonomic diversity, in ecological risk assessment for six freshwater constructed wetlands in a rapidly urbanizing watershed with diverse land uses. Sediment samples were collected summer, 2015 and 2017, and late summer and early fall in 2016 to conduct plant ecotoxicological …

Characterizing Patterns Of Genomic Variation In The Threatened Utah Prairie Dog: Implications For Conservation And Management, Rachael M. Giglio, Tonie E. Rocke, Jorge E. Osorio, Emily Latch

Biological Sciences Faculty Articles

Utah prairie dogs (Cynomys parvidens) are federally threatened due to eradication campaigns, habitat destruction, and outbreaks of plague. Today, Utah prairie dogs exist in small, isolated populations, making them less demographically stable and more susceptible to erosion of genetic variation by genetic drift. We characterized patterns of genetic structure at neutral and putatively adaptive loci in order to evaluate the relative effects of genetic drift and local adaptation on population divergence. We sampled individuals across the Utah prairie dog species range and generated 2,955 single nucleotide polymorphisms (SNPs) using double digest restriction site associated DNA sequencing (ddRAD). Genetic …

Exploring Anatomic Variants To Enhance Anatomy Teaching: Musculus Sternalis, Andrew J. Petto, David E. Zimmerman, Elizabeth K. Johnson, Lucas Gauthier, James T. Menor, Nicholas Wohkittel

Biological Sciences Faculty Articles

The opportunity to encounter and appreciate the range of human variation in anatomic structures—and its potential impact on related structures, function, and treatment—is one of the chief benefits of cadaveric dissection for students in clinical preprofessional programs. The dissection lab is also where students can examine unusual anatomic variants that may not be included in their textbooks, lab manuals, or other course materials. For students specializing in physical medicine, awareness and understanding of muscle variants has a practical relevance to their preparations for clinical practice. In a routine dissection of the superficial chest muscles, graduate students in a human gross …

Evidence For Adaptive Introgression Of Exons Across A Hybrid Swarm In Deer, Emily Latch, Margaret Haines, Gordon Luikart, Stephen Amish, Seth Smith

Biological Sciences Faculty Articles

Background: Secondary contact between closely related lineages can result in a variety of outcomes, including hybridization, depending upon the strength of reproductive barriers. By examining the extent to which different parts of the genome introgress, it is possible to infer the strength of selection and gain insight into the evolutionary trajectory of lineages. Following secondary contact approximately 8000 years ago in the Pacific Northwest, mule deer (Odocoileus hemionus hemionus) and black-tailed deer (O. h. columbianus) formed a hybrid swarm along the Cascade mountain range despite substantial differences in body size (up to two times) and habitat preference. In this study, …

Diverse Microbial Communities Hosted By The Model Carnivorous Pitcher Plant Sarracenia Purpurea: Analysis Of Both Bacterial And Eukaryotic Composition Across Distinct Host Plant Populations, Jacob J. Grothjan, Erica B. Young

Biological Sciences Faculty Articles

Background. The pitcher plant Sarracenia purpurea supplements nutrient acquisition through carnivory, capturing insect prey which are digested by a food web community of eukaryotes and bacteria. While the food web invertebrates are well studied, and some recent studies have characterized bacteria, detailed genetic analysis of eukaryotic diversity is lacking. This study aimed to compare eukaryotic and bacterial composition and diversity of pitcher communities within and between populations of host plants in nearby but distinct wetland habitats, and to characterize microbial functions across populations and in comparison with another freshwater community.

Methods. Pitcher fluid was sampled from the two …

Mitochondrial Voltage-Dependent Anion Channel Protein Por1 Positively Regulates The Nuclear Localization Of Saccharomyces Cerevisiae Amp-Activated Protein Kinase, Aishwarya Shevade, Vera Strogolova, Marianna Orlova, Chay Teng Yeo, Sergei Kuchin

Biological Sciences Faculty Articles

ABSTRACT Snf1 protein kinase of the yeast Saccharomyces cerevisiae is a member of the highly conserved eukaryotic AMP-activated protein kinase (AMPK) family, which is involved in regulating responses to energy limitation. Under conditions of carbon/energy stress, such as during glucose depletion, Snf1 is catalytically activated and enriched in the nucleus to regulate transcription. Snf1 catalytic activation requires phosphorylation of its conserved activation loop threonine (Thr210) by upstream kinases. Catalytic activation is also a prerequisite for Snf1’s subsequent nuclear enrichment, a process that is mediated by Gal83, one of three alternate β-subunits of the Snf1 kinase complex. We previously reported …

Analysis Of Social-Ecological Dynamics Driving Conflict In Linked Surface-Groundwater Systems, Stephen Andrew Mcguire, Timothy J. Ehlinger

Biological Sciences Faculty Articles

Conflict over water resources emerges from complex interactions among biophysical, social, and economic processes operating at multiple scales. Competing use of linked surface-groundwater (LSGW) resources is an excellent yet relatively unexplored example of such conflict. Dynamic circumstances surrounding the contested installation of a high capacity municipal well in southeastern Wisconsin were examined through the theoretical lens of adaptive governance using document analysis and open-ended key stakeholder interviews. A framework analysis method extracted 16 controlling variables from the qualitative data. These controlling variables were placed on a threshold matrix at their appropriate geospatial scale (Property, Watershed, State) and process domain (Biophysical, …

Female Mate Choice Of Male Signals Is Unlikely To Promote Ecological Adaptation In Enchenopa Treehoppers (Hemiptera: Membracidae), Kasey Fowler-Finn, Joseph T. Kilmer, Daniel C. Cruz, Rafael Rodriguez

Biological Sciences Faculty Articles

A key question in speciation research is how ecological and sexual divergence arise and interact. We tested the hypothesis that mate choice causes local adaptation and ecological divergence using the rationale that the performance~signal trait relationship should parallel the attractiveness~signal trait relationship. We used female fecundity as a measure of ecological performance. We used a species in the Enchenopa binotata treehopper complex, wherein speciation involves adaptation to novel environments and divergence in sexual communication. We used a full-sibling, split-family rearing design to estimate genetic correlations (r_G) between fecundity and signal traits, and compared those relationships against population-level …

Characterization Of 2-(2-Nitro-4-Trifluoromethylbenzoyl)- 1,3-Cyclohexanedione Resistance In Pyomelanogenic Pseudomonas Aeruginosa Dkn343, Laura M. Ketelboeter, Sonia L. Bardy

Biological Sciences Faculty Articles

Pyomelanin is a reddish-brown pigment that provides bacteria and fungi protection from oxidative stress, and is reported to contribute to infection persistence. Production of this pigment can be inhibited by the anti-virulence agent 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3- cyclohexanedione (NTBC). The Pseudomonas aeruginosa clinical isolate DKN343 exhibited high levels of resistance to NTBC, and the mechanism of pyomelanin production in this strain was uncharacterized. We determined that pyomelanin production in the clinical Pseudomonas aeruginosa isolate DKN343 was due to a loss of function in homogentisate 1,2- dioxygenase (HmgA). Several potential resistance mechanisms were investigated, and the MexAB-OprM efflux pump is required for resistance to …